Semi-Quantification of Lectins in Rice (<i>Oryza sativa</i> L.) Genotypes via Hemagglutination
Lectins are unique glycoproteins that react with specific sugar residues on cell surfaces resulting in agglutination. They offer enormous applications in therapeutics, diagnostics, medicine, and agriculture. Rice lectins are naturally expressed during biotic and abiotic stresses suggesting their imp...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/10/1899 |
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| author | Haseena Gulzar Muhammad Asif Nawaz Asad Jan Farhat Ali Khan Sumaira Naz Muhammad Zahoor Dil Naz Riaz Ullah Essam A. Ali Hidayat Hussain |
| author_facet | Haseena Gulzar Muhammad Asif Nawaz Asad Jan Farhat Ali Khan Sumaira Naz Muhammad Zahoor Dil Naz Riaz Ullah Essam A. Ali Hidayat Hussain |
| author_sort | Haseena Gulzar |
| collection | DOAJ |
| description | Lectins are unique glycoproteins that react with specific sugar residues on cell surfaces resulting in agglutination. They offer enormous applications in therapeutics, diagnostics, medicine, and agriculture. Rice lectins are naturally expressed during biotic and abiotic stresses suggesting their importance in stress resistance physiology. The objective of this study was to determine the presence and relative concentration of lectins in different accessions of rice obtained from IABGR/NARC Islamabad mainly originated from Pakistan. About 210 rice accessions including 02 local varieties and 05 transgenic seeds were screened for seed lectins using a hemagglutination (HA) assay with 5% Californian bred rabbits’ erythrocytes. A protein concentration of 3–8 mg/100 mg of seed flour was measured for all the rice accessions; the highest was 8.03 mg for accession 7600, while the lowest noted was 3.05 mg for accession 7753. Out of 210 accessions, 106 showed the highest HA activity. These 106 genotypes were further screened for titer analysis and specific activity. The highest titer and specific activity were observed for accession 7271 as 1024 and 236 hemagglutination unit (HAU), respectively. The selected accessions’ relative affinity and HA capability were evaluated using blood from four different sources: human, broiler chicken, local rabbit, and Californian-breed rabbit. The highest HA activity was observed with Californian-breed rabbit RBCs. The lectin assay was stable for about 1–2 h. After the required investigations, the accessions with higher lectin concentration and HA capability could be used as a readily available source of lectins for further characterization and utilization in crop improvement programs. |
| first_indexed | 2024-03-09T04:40:19Z |
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| id | doaj.art-b8718f77c1a14f05ab773e43a2b6c1c0 |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T04:40:19Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-b8718f77c1a14f05ab773e43a2b6c1c02023-12-03T13:22:51ZengMDPI AGAgronomy2073-43952021-09-011110189910.3390/agronomy11101899Semi-Quantification of Lectins in Rice (<i>Oryza sativa</i> L.) Genotypes via HemagglutinationHaseena Gulzar0Muhammad Asif Nawaz1Asad Jan2Farhat Ali Khan3Sumaira Naz4Muhammad Zahoor5Dil Naz6Riaz Ullah7Essam A. Ali8Hidayat Hussain9Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal Dir Upper, Peshawar 18000, Khyber Pakhtunkhwa, PakistanDepartment of Biotechnology, Shaheed Benazir Bhutto University, Sheringal Dir Upper, Peshawar 18000, Khyber Pakhtunkhwa, PakistanInstitute of Biotechnology and Genetic Engineering, Agriculture University Peshawar, Peshawar 18000, Khyber Pakhtunkhwa, PakistanDepartment of Pharmacy, Shaheed Benazir Bhutto University, Sheringal Dir Upper, Peshawar 18000, Khyber Pakhtunkhwa, PakistanDepartment of Biochemistry, University of Malakand, Chakdara Dir Lower, Malakand 18800, Khyber Pakhtunkhwa, PakistanDepartment of Biochemistry, University of Malakand, Chakdara Dir Lower, Malakand 18800, Khyber Pakhtunkhwa, PakistanDepartment of Zoology, University of Malakand, Chakdara Dir Lower, Malakand 18800, Khyber Pakhtunkhwa, PakistanDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle (Salle), GermanyLectins are unique glycoproteins that react with specific sugar residues on cell surfaces resulting in agglutination. They offer enormous applications in therapeutics, diagnostics, medicine, and agriculture. Rice lectins are naturally expressed during biotic and abiotic stresses suggesting their importance in stress resistance physiology. The objective of this study was to determine the presence and relative concentration of lectins in different accessions of rice obtained from IABGR/NARC Islamabad mainly originated from Pakistan. About 210 rice accessions including 02 local varieties and 05 transgenic seeds were screened for seed lectins using a hemagglutination (HA) assay with 5% Californian bred rabbits’ erythrocytes. A protein concentration of 3–8 mg/100 mg of seed flour was measured for all the rice accessions; the highest was 8.03 mg for accession 7600, while the lowest noted was 3.05 mg for accession 7753. Out of 210 accessions, 106 showed the highest HA activity. These 106 genotypes were further screened for titer analysis and specific activity. The highest titer and specific activity were observed for accession 7271 as 1024 and 236 hemagglutination unit (HAU), respectively. The selected accessions’ relative affinity and HA capability were evaluated using blood from four different sources: human, broiler chicken, local rabbit, and Californian-breed rabbit. The highest HA activity was observed with Californian-breed rabbit RBCs. The lectin assay was stable for about 1–2 h. After the required investigations, the accessions with higher lectin concentration and HA capability could be used as a readily available source of lectins for further characterization and utilization in crop improvement programs.https://www.mdpi.com/2073-4395/11/10/1899rice<i>Oryza sativa</i> L.lectinshemagglutination assayrabbit RBCstiter values |
| spellingShingle | Haseena Gulzar Muhammad Asif Nawaz Asad Jan Farhat Ali Khan Sumaira Naz Muhammad Zahoor Dil Naz Riaz Ullah Essam A. Ali Hidayat Hussain Semi-Quantification of Lectins in Rice (<i>Oryza sativa</i> L.) Genotypes via Hemagglutination Agronomy rice <i>Oryza sativa</i> L. lectins hemagglutination assay rabbit RBCs titer values |
| title | Semi-Quantification of Lectins in Rice (<i>Oryza sativa</i> L.) Genotypes via Hemagglutination |
| title_full | Semi-Quantification of Lectins in Rice (<i>Oryza sativa</i> L.) Genotypes via Hemagglutination |
| title_fullStr | Semi-Quantification of Lectins in Rice (<i>Oryza sativa</i> L.) Genotypes via Hemagglutination |
| title_full_unstemmed | Semi-Quantification of Lectins in Rice (<i>Oryza sativa</i> L.) Genotypes via Hemagglutination |
| title_short | Semi-Quantification of Lectins in Rice (<i>Oryza sativa</i> L.) Genotypes via Hemagglutination |
| title_sort | semi quantification of lectins in rice i oryza sativa i l genotypes via hemagglutination |
| topic | rice <i>Oryza sativa</i> L. lectins hemagglutination assay rabbit RBCs titer values |
| url | https://www.mdpi.com/2073-4395/11/10/1899 |
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