Comparative analysis of the biocompatibility of endothelial cells on surfaces treated by thermal plasma and cold atmospheric plasma
Abstract In recent years, cold atmospheric plasma (CAP) is used for surface disinfection. However, little is known about its ability to improve biocompatibility of metallic surfaces when compared to thermal plasma methods. In this context, the study aimed to evaluate the response of human endothelia...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academia Brasileira de Ciências
2023-10-01
|
| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652023000400601&tlng=en |
| _version_ | 1797652476904603648 |
|---|---|
| author | ALAN MAX T. DE SOUZA JANINE KARLA F. DA SILVA BRAZ GABRIEL M. MARTINS JUSSIER DE OLIVEIRA VITORIANO AURÉLIO G.A. NETO DAVID M. NERY VLADIMIR G. SABINO EUDES E. DE SOUZA LUCENA HUGO ALEXANDRE DE OLIVEIRA ROCHA CARLOS AUGUSTO G. BARBOZA CLODOMIRO A. JÚNIOR CARLOS EDUARDO B. DE MOURA |
| author_facet | ALAN MAX T. DE SOUZA JANINE KARLA F. DA SILVA BRAZ GABRIEL M. MARTINS JUSSIER DE OLIVEIRA VITORIANO AURÉLIO G.A. NETO DAVID M. NERY VLADIMIR G. SABINO EUDES E. DE SOUZA LUCENA HUGO ALEXANDRE DE OLIVEIRA ROCHA CARLOS AUGUSTO G. BARBOZA CLODOMIRO A. JÚNIOR CARLOS EDUARDO B. DE MOURA |
| author_sort | ALAN MAX T. DE SOUZA |
| collection | DOAJ |
| description | Abstract In recent years, cold atmospheric plasma (CAP) is used for surface disinfection. However, little is known about its ability to improve biocompatibility of metallic surfaces when compared to thermal plasma methods. In this context, the study aimed to evaluate the response of human endothelial cells (Ea.hy926) on titanium surfaces treated by non-thermal plasma method and thermal plasma method under nitriding atmosphere. The wettability was characterized by the sessile drop method, the topography and roughness were evaluated by atomic force microscopy (AFM), and the microstructure by grazing angle X-ray diffraction (GIXRD). Endothelial cells were cultured and evaluated for morphology by scanning electron microscopy and viability by an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. CAP treatment reduced the contact angle of the Ti surface (13.43° ± 1.48; p<0.05), increasing hydrophilicity. Rz roughness was higher on the nitrided surface (220.44±20.30; p< 0.001) compared to the CAP treated surfaces (83.29 ± 11.61; p< 0.001) and polished (75.98 ±34.21a); p<0.001). The different applied plasma treatments created different titanium surfaces improving the biocompatibility of endothelial cells, however CAP results demonstrate its potential for biomedical applications, considering the low cost and ease of use of the technique, allowing surface treatments before clinical procedures. |
| first_indexed | 2024-03-11T16:30:29Z |
| format | Article |
| id | doaj.art-b872869fb3e845c192021a089ea635c3 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-03-11T16:30:29Z |
| publishDate | 2023-10-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-b872869fb3e845c192021a089ea635c32023-10-24T07:38:15ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902023-10-0195310.1590/0001-3765202320220865Comparative analysis of the biocompatibility of endothelial cells on surfaces treated by thermal plasma and cold atmospheric plasmaALAN MAX T. DE SOUZAhttps://orcid.org/0000-0002-5255-2486JANINE KARLA F. DA SILVA BRAZhttps://orcid.org/0000-0002-9570-6465GABRIEL M. MARTINShttps://orcid.org/0000-0001-6489-4187JUSSIER DE OLIVEIRA VITORIANOhttps://orcid.org/0000-0002-9357-2088AURÉLIO G.A. NETOhttps://orcid.org/0000-0002-6891-1930DAVID M. NERYhttps://orcid.org/0000-0001-7541-7175VLADIMIR G. SABINOhttps://orcid.org/0000-0003-0804-0675EUDES E. DE SOUZA LUCENAhttps://orcid.org/0000-0003-3119-7822HUGO ALEXANDRE DE OLIVEIRA ROCHAhttps://orcid.org/0000-0003-2252-1221CARLOS AUGUSTO G. BARBOZAhttps://orcid.org/0000-0003-1979-9919CLODOMIRO A. JÚNIORhttps://orcid.org/0000-0002-5547-5922CARLOS EDUARDO B. DE MOURAhttps://orcid.org/0000-0002-7960-5373Abstract In recent years, cold atmospheric plasma (CAP) is used for surface disinfection. However, little is known about its ability to improve biocompatibility of metallic surfaces when compared to thermal plasma methods. In this context, the study aimed to evaluate the response of human endothelial cells (Ea.hy926) on titanium surfaces treated by non-thermal plasma method and thermal plasma method under nitriding atmosphere. The wettability was characterized by the sessile drop method, the topography and roughness were evaluated by atomic force microscopy (AFM), and the microstructure by grazing angle X-ray diffraction (GIXRD). Endothelial cells were cultured and evaluated for morphology by scanning electron microscopy and viability by an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. CAP treatment reduced the contact angle of the Ti surface (13.43° ± 1.48; p<0.05), increasing hydrophilicity. Rz roughness was higher on the nitrided surface (220.44±20.30; p< 0.001) compared to the CAP treated surfaces (83.29 ± 11.61; p< 0.001) and polished (75.98 ±34.21a); p<0.001). The different applied plasma treatments created different titanium surfaces improving the biocompatibility of endothelial cells, however CAP results demonstrate its potential for biomedical applications, considering the low cost and ease of use of the technique, allowing surface treatments before clinical procedures.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652023000400601&tlng=enAngiogenesiscold atmospheric plasmanitridingtitanium |
| spellingShingle | ALAN MAX T. DE SOUZA JANINE KARLA F. DA SILVA BRAZ GABRIEL M. MARTINS JUSSIER DE OLIVEIRA VITORIANO AURÉLIO G.A. NETO DAVID M. NERY VLADIMIR G. SABINO EUDES E. DE SOUZA LUCENA HUGO ALEXANDRE DE OLIVEIRA ROCHA CARLOS AUGUSTO G. BARBOZA CLODOMIRO A. JÚNIOR CARLOS EDUARDO B. DE MOURA Comparative analysis of the biocompatibility of endothelial cells on surfaces treated by thermal plasma and cold atmospheric plasma Anais da Academia Brasileira de Ciências Angiogenesis cold atmospheric plasma nitriding titanium |
| title | Comparative analysis of the biocompatibility of endothelial cells on surfaces treated by thermal plasma and cold atmospheric plasma |
| title_full | Comparative analysis of the biocompatibility of endothelial cells on surfaces treated by thermal plasma and cold atmospheric plasma |
| title_fullStr | Comparative analysis of the biocompatibility of endothelial cells on surfaces treated by thermal plasma and cold atmospheric plasma |
| title_full_unstemmed | Comparative analysis of the biocompatibility of endothelial cells on surfaces treated by thermal plasma and cold atmospheric plasma |
| title_short | Comparative analysis of the biocompatibility of endothelial cells on surfaces treated by thermal plasma and cold atmospheric plasma |
| title_sort | comparative analysis of the biocompatibility of endothelial cells on surfaces treated by thermal plasma and cold atmospheric plasma |
| topic | Angiogenesis cold atmospheric plasma nitriding titanium |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652023000400601&tlng=en |
| work_keys_str_mv | AT alanmaxtdesouza comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT janinekarlafdasilvabraz comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT gabrielmmartins comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT jussierdeoliveiravitoriano comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT aurelioganeto comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT davidmnery comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT vladimirgsabino comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT eudesedesouzalucena comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT hugoalexandredeoliveirarocha comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT carlosaugustogbarboza comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT clodomiroajunior comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma AT carloseduardobdemoura comparativeanalysisofthebiocompatibilityofendothelialcellsonsurfacestreatedbythermalplasmaandcoldatmosphericplasma |