Evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic disease
Objective: The aim of this study was to evaluate the effectiveness of mature red cell and reticulocyte parameters to identify three conditions: iron deficiency anemia, anemia of chronic disease, and anemia of chronic disease associated with absolute iron deficiency. Methods: Peripheral blood cells f...
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| Format: | Article |
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Elsevier
2015-04-01
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| Series: | Revista Brasileira de Hematologia e Hemoterapia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000200077&lng=en&tlng=en |
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| author | Ana Beatriz Barbosa Torino Maria de Fátima Pererira Gilberti Edvilson da Costa Gisélia Aparecida Freire de Lima Helena Zerlotti Wolf Grotto |
| author_facet | Ana Beatriz Barbosa Torino Maria de Fátima Pererira Gilberti Edvilson da Costa Gisélia Aparecida Freire de Lima Helena Zerlotti Wolf Grotto |
| author_sort | Ana Beatriz Barbosa Torino |
| collection | DOAJ |
| description | Objective: The aim of this study was to evaluate the effectiveness of mature red cell and reticulocyte parameters to identify three conditions: iron deficiency anemia, anemia of chronic disease, and anemia of chronic disease associated with absolute iron deficiency. Methods: Peripheral blood cells from 117 adult patients with anemia were classified accord- ing to iron status, inflammation, and hemoglobinopathies as: iron deficiency anemia (n = 42), anemia of chronic disease (n = 28), anemia of chronic disease associated with iron deficiency anemia (n = 22), and heterozygous β-thalassemia (n = 25). The percentage of microcytic eryth-rocytes, hypochromic erythrocytes, and the levels of hemoglobin in both reticulocytes and mature red cells were determined. Receiver operating characteristic analysis was used to evaluate the accuracy of the parameters in differentiating anemia. Results: There was no difference between the groups of iron deficiency and anemia of chronic disease associated with absolute iron deficiency for any of the parameters. The percentage of hypochromic erythrocytes was the best parameter to identify absolute iron deficiency in patients with anemia of chronic disease (area under curve = 0.785; 95% confidence interval: 0.661-0.909 with sensitivity of 72.7%, and specificity of 70.4%; cut-off value 1.8%). The formula microcytic erythrocyte count minus hypochromic erythrocyte count was very accurate to differentiate iron deficiency anemia from heterozygous β-thalassemia (area under curve = 0.977; 95% confidence interval: 0.950-1.005 with a sensitivity of 96.2%, and specificity of 92.7%; cut-off value 13.8). Conclusion: The erythrocyte and reticulocyte indices are moderately good to identify absolute iron deficiency in patients with anemia of chronic disease. |
| first_indexed | 2024-04-13T21:08:58Z |
| format | Article |
| id | doaj.art-b8746507920c48c68af48f1adcd33009 |
| institution | Directory Open Access Journal |
| issn | 1806-0870 |
| language | English |
| last_indexed | 2024-04-13T21:08:58Z |
| publishDate | 2015-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Revista Brasileira de Hematologia e Hemoterapia |
| spelling | doaj.art-b8746507920c48c68af48f1adcd330092022-12-22T02:29:53ZengElsevierRevista Brasileira de Hematologia e Hemoterapia1806-08702015-04-01372778110.1016/j.bjhh.2015.02.004S1516-84842015000200077Evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic diseaseAna Beatriz Barbosa TorinoMaria de Fátima Pererira GilbertiEdvilson da CostaGisélia Aparecida Freire de LimaHelena Zerlotti Wolf GrottoObjective: The aim of this study was to evaluate the effectiveness of mature red cell and reticulocyte parameters to identify three conditions: iron deficiency anemia, anemia of chronic disease, and anemia of chronic disease associated with absolute iron deficiency. Methods: Peripheral blood cells from 117 adult patients with anemia were classified accord- ing to iron status, inflammation, and hemoglobinopathies as: iron deficiency anemia (n = 42), anemia of chronic disease (n = 28), anemia of chronic disease associated with iron deficiency anemia (n = 22), and heterozygous β-thalassemia (n = 25). The percentage of microcytic eryth-rocytes, hypochromic erythrocytes, and the levels of hemoglobin in both reticulocytes and mature red cells were determined. Receiver operating characteristic analysis was used to evaluate the accuracy of the parameters in differentiating anemia. Results: There was no difference between the groups of iron deficiency and anemia of chronic disease associated with absolute iron deficiency for any of the parameters. The percentage of hypochromic erythrocytes was the best parameter to identify absolute iron deficiency in patients with anemia of chronic disease (area under curve = 0.785; 95% confidence interval: 0.661-0.909 with sensitivity of 72.7%, and specificity of 70.4%; cut-off value 1.8%). The formula microcytic erythrocyte count minus hypochromic erythrocyte count was very accurate to differentiate iron deficiency anemia from heterozygous β-thalassemia (area under curve = 0.977; 95% confidence interval: 0.950-1.005 with a sensitivity of 96.2%, and specificity of 92.7%; cut-off value 13.8). Conclusion: The erythrocyte and reticulocyte indices are moderately good to identify absolute iron deficiency in patients with anemia of chronic disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000200077&lng=en&tlng=enAutomationAnemiairon-deficiency Erythrocyte indices Reticulocytes Erythropoiesis |
| spellingShingle | Ana Beatriz Barbosa Torino Maria de Fátima Pererira Gilberti Edvilson da Costa Gisélia Aparecida Freire de Lima Helena Zerlotti Wolf Grotto Evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic disease Revista Brasileira de Hematologia e Hemoterapia Automation Anemia iron-deficiency Erythrocyte indices Reticulocytes Erythropoiesis |
| title | Evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic disease |
| title_full | Evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic disease |
| title_fullStr | Evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic disease |
| title_full_unstemmed | Evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic disease |
| title_short | Evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic disease |
| title_sort | evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic disease |
| topic | Automation Anemia iron-deficiency Erythrocyte indices Reticulocytes Erythropoiesis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000200077&lng=en&tlng=en |
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