High-Throughput Detection and Characterization of Antimicrobial Resistant <i>Enterococcus</i> sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding Operations
Antimicrobial resistant enteric bacteria can easily contaminate the environment and other vehicles through the deposition of human and animal feces. In turn, humans can be exposed to these antimicrobial resistant (AMR) bacteria through contaminated food products and/or contaminated drinking water. A...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/7/890 |
| _version_ | 1797563170355675136 |
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| author | Jennifer Anders Bledar Bisha |
| author_facet | Jennifer Anders Bledar Bisha |
| author_sort | Jennifer Anders |
| collection | DOAJ |
| description | Antimicrobial resistant enteric bacteria can easily contaminate the environment and other vehicles through the deposition of human and animal feces. In turn, humans can be exposed to these antimicrobial resistant (AMR) bacteria through contaminated food products and/or contaminated drinking water. As wildlife are firmly established as reservoirs of AMR bacteria and serve as potential vectors in the constant spread of AMR, limiting contact between wildlife and livestock and effective tracking of AMR bacteria can help minimize AMR dissemination to humans through contaminated food and water. <i>Enterococcus</i> spp., which are known opportunistic pathogens, constantly found in gastrointestinal tracts of mammalian and avian species, swiftly evolve and cultivate AMR genotypes and phenotypes, which they easily distribute to other bacteria, including several major bacterial pathogens. In this study, we evaluated the use of high throughput detection and characterization of enterococci from wildlife [European starlings (<i>Sturnus vulgaris</i>)] by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) following culture-based isolation. MALDI-TOF MS successfully identified 658 <i>Enterococcus</i> spp. isolates out of 718 presumptive isolates collected from gastrointestinal tracts of European starlings, which were captured near livestock operations in Colorado, Iowa, Kansas, Missouri, and Texas; antimicrobial susceptibility testing was then performed using 13 clinically significant antibiotics. |
| first_indexed | 2024-03-10T18:38:50Z |
| format | Article |
| id | doaj.art-b8774107c49b4073b3aaff27610c87a7 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T18:38:50Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-b8774107c49b4073b3aaff27610c87a72023-11-20T06:02:28ZengMDPI AGFoods2304-81582020-07-019789010.3390/foods9070890High-Throughput Detection and Characterization of Antimicrobial Resistant <i>Enterococcus</i> sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding OperationsJennifer Anders0Bledar Bisha1Department of Animal Science, University of Wyoming, Laramie, WY 82071, USADepartment of Animal Science, University of Wyoming, Laramie, WY 82071, USAAntimicrobial resistant enteric bacteria can easily contaminate the environment and other vehicles through the deposition of human and animal feces. In turn, humans can be exposed to these antimicrobial resistant (AMR) bacteria through contaminated food products and/or contaminated drinking water. As wildlife are firmly established as reservoirs of AMR bacteria and serve as potential vectors in the constant spread of AMR, limiting contact between wildlife and livestock and effective tracking of AMR bacteria can help minimize AMR dissemination to humans through contaminated food and water. <i>Enterococcus</i> spp., which are known opportunistic pathogens, constantly found in gastrointestinal tracts of mammalian and avian species, swiftly evolve and cultivate AMR genotypes and phenotypes, which they easily distribute to other bacteria, including several major bacterial pathogens. In this study, we evaluated the use of high throughput detection and characterization of enterococci from wildlife [European starlings (<i>Sturnus vulgaris</i>)] by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) following culture-based isolation. MALDI-TOF MS successfully identified 658 <i>Enterococcus</i> spp. isolates out of 718 presumptive isolates collected from gastrointestinal tracts of European starlings, which were captured near livestock operations in Colorado, Iowa, Kansas, Missouri, and Texas; antimicrobial susceptibility testing was then performed using 13 clinically significant antibiotics.https://www.mdpi.com/2304-8158/9/7/890antimicrobial resistance<i>Enterococcus</i> spp.high throughput detectionmatrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)culture-based isolationsusceptibility testing |
| spellingShingle | Jennifer Anders Bledar Bisha High-Throughput Detection and Characterization of Antimicrobial Resistant <i>Enterococcus</i> sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding Operations Foods antimicrobial resistance <i>Enterococcus</i> spp. high throughput detection matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) culture-based isolation susceptibility testing |
| title | High-Throughput Detection and Characterization of Antimicrobial Resistant <i>Enterococcus</i> sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding Operations |
| title_full | High-Throughput Detection and Characterization of Antimicrobial Resistant <i>Enterococcus</i> sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding Operations |
| title_fullStr | High-Throughput Detection and Characterization of Antimicrobial Resistant <i>Enterococcus</i> sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding Operations |
| title_full_unstemmed | High-Throughput Detection and Characterization of Antimicrobial Resistant <i>Enterococcus</i> sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding Operations |
| title_short | High-Throughput Detection and Characterization of Antimicrobial Resistant <i>Enterococcus</i> sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding Operations |
| title_sort | high throughput detection and characterization of antimicrobial resistant i enterococcus i sp isolates from gi tracts of european starlings visiting concentrated animal feeding operations |
| topic | antimicrobial resistance <i>Enterococcus</i> spp. high throughput detection matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) culture-based isolation susceptibility testing |
| url | https://www.mdpi.com/2304-8158/9/7/890 |
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