Molecular Detection of Tick-Borne Pathogens in American Bison (<i>Bison bison</i>) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico
American bison (<i>Bison bison</i>) is listed as near-threatened and in danger of extinction in Mexico. Recent studies have demonstrated the presence of several emerging pathogens at the Janos Biosphere Reserve (JBR), inhabited by one wild herd of American bison. Blood samples were colle...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/11/1428 |
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| author | Diana M. Beristain-Ruiz Cuauhcihuatl Vital-García Julio V. Figueroa-Millán José J. Lira-Amaya Javier A. Garza-Hernández Juan R. Sánchez-Ayala Samuel Flores-Ceballos Carlos A. Rodríguez-Alarcón Martha P. Olivas-Sánchez Gabriel Pons-Monarrez |
| author_facet | Diana M. Beristain-Ruiz Cuauhcihuatl Vital-García Julio V. Figueroa-Millán José J. Lira-Amaya Javier A. Garza-Hernández Juan R. Sánchez-Ayala Samuel Flores-Ceballos Carlos A. Rodríguez-Alarcón Martha P. Olivas-Sánchez Gabriel Pons-Monarrez |
| author_sort | Diana M. Beristain-Ruiz |
| collection | DOAJ |
| description | American bison (<i>Bison bison</i>) is listed as near-threatened and in danger of extinction in Mexico. Recent studies have demonstrated the presence of several emerging pathogens at the Janos Biosphere Reserve (JBR), inhabited by one wild herd of American bison. Blood samples were collected from 26 American bison in the JBR. We tested for the presence of <i>Anaplasma marginale</i>, <i>Babesia bigemina</i>, <i>B. bovis</i>, <i>Borrelia burgdorferi sensu lato</i>, and <i>Rickettsia rickettsii</i> DNA using nested and semi-nested PCR protocols performing duplicates in two different laboratories. Results showed three animals (11.5%) positive for <i>B. burgdorferi s. l.</i>, three more (11.5%) for <i>Rickettsia rickettsii,</i> and four (19.2%) for <i>B. bovis</i>. Two individuals were co-infected with <i>B. burgdorferi s. l.</i> and <i>B. bovis</i>. We found no animals positive for <i>A. marginale</i> and <i>B. bigemina</i>. This is the first report in America of <i>R. rickettsii</i> in American bison. American bison has been described as an important reservoir for pathogens of zoonotic and veterinary importance; thus, the presence of tick-borne pathogen DNA in the JBR American bison indicates the importance of continuous wildlife health surveys. |
| first_indexed | 2024-03-10T05:10:17Z |
| format | Article |
| id | doaj.art-b87901e7d9fd41ce984895dd0e11a605 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T05:10:17Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-b87901e7d9fd41ce984895dd0e11a6052023-11-23T00:53:10ZengMDPI AGPathogens2076-08172021-11-011011142810.3390/pathogens10111428Molecular Detection of Tick-Borne Pathogens in American Bison (<i>Bison bison</i>) at El Uno Ecological Reserve, Janos, Chihuahua, MexicoDiana M. Beristain-Ruiz0Cuauhcihuatl Vital-García1Julio V. Figueroa-Millán2José J. Lira-Amaya3Javier A. Garza-Hernández4Juan R. Sánchez-Ayala5Samuel Flores-Ceballos6Carlos A. Rodríguez-Alarcón7Martha P. Olivas-Sánchez8Gabriel Pons-Monarrez9Departamento de Ciencias Veterinarias, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente y Estocolmo s/n Colonia Progresista AP 1729-D Cd. Juárez, Chihuahua CP 32310, MexicoDepartamento de Ciencias Veterinarias, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente y Estocolmo s/n Colonia Progresista AP 1729-D Cd. Juárez, Chihuahua CP 32310, MexicoCENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Cuernavaca-Cuautla 8534, Progreso, Jiutepec CP 62574, MexicoCENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Cuernavaca-Cuautla 8534, Progreso, Jiutepec CP 62574, MexicoDepartamento de Ciencias Químico-Biológicas, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente y Estocolmo s/n Colonia Progresista AP 1729-D Cd. Juárez, Chihuahua CP 32310, MexicoFacultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, México D. F. CP 04510, MexicoFacultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, México D. F. CP 04510, MexicoDepartamento de Ciencias Veterinarias, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente y Estocolmo s/n Colonia Progresista AP 1729-D Cd. Juárez, Chihuahua CP 32310, MexicoDepartamento de Ciencias Químico-Biológicas, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente y Estocolmo s/n Colonia Progresista AP 1729-D Cd. Juárez, Chihuahua CP 32310, MexicoDepartamento de Ciencias Veterinarias, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente y Estocolmo s/n Colonia Progresista AP 1729-D Cd. Juárez, Chihuahua CP 32310, MexicoAmerican bison (<i>Bison bison</i>) is listed as near-threatened and in danger of extinction in Mexico. Recent studies have demonstrated the presence of several emerging pathogens at the Janos Biosphere Reserve (JBR), inhabited by one wild herd of American bison. Blood samples were collected from 26 American bison in the JBR. We tested for the presence of <i>Anaplasma marginale</i>, <i>Babesia bigemina</i>, <i>B. bovis</i>, <i>Borrelia burgdorferi sensu lato</i>, and <i>Rickettsia rickettsii</i> DNA using nested and semi-nested PCR protocols performing duplicates in two different laboratories. Results showed three animals (11.5%) positive for <i>B. burgdorferi s. l.</i>, three more (11.5%) for <i>Rickettsia rickettsii,</i> and four (19.2%) for <i>B. bovis</i>. Two individuals were co-infected with <i>B. burgdorferi s. l.</i> and <i>B. bovis</i>. We found no animals positive for <i>A. marginale</i> and <i>B. bigemina</i>. This is the first report in America of <i>R. rickettsii</i> in American bison. American bison has been described as an important reservoir for pathogens of zoonotic and veterinary importance; thus, the presence of tick-borne pathogen DNA in the JBR American bison indicates the importance of continuous wildlife health surveys.https://www.mdpi.com/2076-0817/10/11/1428<i>Bison bison</i>ticksNorth American prairiestick-borne diseases |
| spellingShingle | Diana M. Beristain-Ruiz Cuauhcihuatl Vital-García Julio V. Figueroa-Millán José J. Lira-Amaya Javier A. Garza-Hernández Juan R. Sánchez-Ayala Samuel Flores-Ceballos Carlos A. Rodríguez-Alarcón Martha P. Olivas-Sánchez Gabriel Pons-Monarrez Molecular Detection of Tick-Borne Pathogens in American Bison (<i>Bison bison</i>) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico Pathogens <i>Bison bison</i> ticks North American prairies tick-borne diseases |
| title | Molecular Detection of Tick-Borne Pathogens in American Bison (<i>Bison bison</i>) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico |
| title_full | Molecular Detection of Tick-Borne Pathogens in American Bison (<i>Bison bison</i>) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico |
| title_fullStr | Molecular Detection of Tick-Borne Pathogens in American Bison (<i>Bison bison</i>) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico |
| title_full_unstemmed | Molecular Detection of Tick-Borne Pathogens in American Bison (<i>Bison bison</i>) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico |
| title_short | Molecular Detection of Tick-Borne Pathogens in American Bison (<i>Bison bison</i>) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico |
| title_sort | molecular detection of tick borne pathogens in american bison i bison bison i at el uno ecological reserve janos chihuahua mexico |
| topic | <i>Bison bison</i> ticks North American prairies tick-borne diseases |
| url | https://www.mdpi.com/2076-0817/10/11/1428 |
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