An isolated retrograde-perfused newborn mouse heart preparation
The Langendorff-perfused model is a powerful tool to study biological responses in the isolated heart in the absence of confounders. The model has been adapted recently to enable study of the isolated mouse heart and the effects of genetic manipulation. Unfortunately, the small size and fragility of...
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Format: | Article |
Language: | English |
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Elsevier
2020-01-01
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Series: | MethodsX |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016120302788 |
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author | Matthew Barajas Peter D. Yim George Gallos Richard J. Levy |
author_facet | Matthew Barajas Peter D. Yim George Gallos Richard J. Levy |
author_sort | Matthew Barajas |
collection | DOAJ |
description | The Langendorff-perfused model is a powerful tool to study biological responses in the isolated heart in the absence of confounders. The model has been adapted recently to enable study of the isolated mouse heart and the effects of genetic manipulation. Unfortunately, the small size and fragility of the mouse heart pose significant challenges, limiting application of the Langendorff model to the study of adult mice. Cardiac development is a complex and dynamic process that is incompletely understood. Thus, establishing an isolated-perfused heart model in the newborn mouse would be an important and necessary advance. Here we present a method to successfully cannulate and perfuse the isolated newborn murine heart. We describe the basic and fundamental physiological characteristics of the ex-vivo retrograde-perfused beating neonatal heart in wild-type C57Bl/6 male mice. Our approach will enable future study of the physiological and pharmacological responses of the isolated immature murine heart to enhance knowledge of how developmental cardiac biology impacts health and disease. • The Langendorff model is a powerful tool to study the heart without confounders. • An isolated-perfused newborn murine heart model has yet to be established. • We demonstrate the first successful isolated neonatal murine heart preparation. |
first_indexed | 2024-12-14T21:13:49Z |
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id | doaj.art-b879f889590941b7b467b72e131dc611 |
institution | Directory Open Access Journal |
issn | 2215-0161 |
language | English |
last_indexed | 2024-12-14T21:13:49Z |
publishDate | 2020-01-01 |
publisher | Elsevier |
record_format | Article |
series | MethodsX |
spelling | doaj.art-b879f889590941b7b467b72e131dc6112022-12-21T22:47:08ZengElsevierMethodsX2215-01612020-01-017101058An isolated retrograde-perfused newborn mouse heart preparationMatthew Barajas0Peter D. Yim1George Gallos2Richard J. Levy3Department of Anesthesiology, Columbia University Medical Center, 622 W. 168th Street, New York, NY 10032, United StatesDepartment of Anesthesiology, Columbia University Medical Center, 622 W. 168th Street, New York, NY 10032, United StatesDepartment of Anesthesiology, Columbia University Medical Center, 622 W. 168th Street, New York, NY 10032, United StatesCorresponding author.; Department of Anesthesiology, Columbia University Medical Center, 622 W. 168th Street, New York, NY 10032, United StatesThe Langendorff-perfused model is a powerful tool to study biological responses in the isolated heart in the absence of confounders. The model has been adapted recently to enable study of the isolated mouse heart and the effects of genetic manipulation. Unfortunately, the small size and fragility of the mouse heart pose significant challenges, limiting application of the Langendorff model to the study of adult mice. Cardiac development is a complex and dynamic process that is incompletely understood. Thus, establishing an isolated-perfused heart model in the newborn mouse would be an important and necessary advance. Here we present a method to successfully cannulate and perfuse the isolated newborn murine heart. We describe the basic and fundamental physiological characteristics of the ex-vivo retrograde-perfused beating neonatal heart in wild-type C57Bl/6 male mice. Our approach will enable future study of the physiological and pharmacological responses of the isolated immature murine heart to enhance knowledge of how developmental cardiac biology impacts health and disease. • The Langendorff model is a powerful tool to study the heart without confounders. • An isolated-perfused newborn murine heart model has yet to be established. • We demonstrate the first successful isolated neonatal murine heart preparation.http://www.sciencedirect.com/science/article/pii/S2215016120302788Cannulation of neonatal mouse heart and establishment of successful isolated neonatal murine heart preparation |
spellingShingle | Matthew Barajas Peter D. Yim George Gallos Richard J. Levy An isolated retrograde-perfused newborn mouse heart preparation MethodsX Cannulation of neonatal mouse heart and establishment of successful isolated neonatal murine heart preparation |
title | An isolated retrograde-perfused newborn mouse heart preparation |
title_full | An isolated retrograde-perfused newborn mouse heart preparation |
title_fullStr | An isolated retrograde-perfused newborn mouse heart preparation |
title_full_unstemmed | An isolated retrograde-perfused newborn mouse heart preparation |
title_short | An isolated retrograde-perfused newborn mouse heart preparation |
title_sort | isolated retrograde perfused newborn mouse heart preparation |
topic | Cannulation of neonatal mouse heart and establishment of successful isolated neonatal murine heart preparation |
url | http://www.sciencedirect.com/science/article/pii/S2215016120302788 |
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