Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19
Within the framework of the current COVID-19 pandemic, there is a race against time to find therapies for the outbreak to be controlled. Since vaccines are still tedious to develop and partially available for low-income countries, passive immunity based on egg-yolk antibodies (IgY) is presented as a...
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.881604/full |
| _version_ | 1818204258007252992 |
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| author | Andres Agurto-Arteaga Astrid Poma-Acevedo Dora Rios-Matos Ricardo Choque-Guevara Ricardo Montesinos-Millán Ángela Montalván Gisela Isasi-Rivas Yudith Cauna-Orocollo María de Grecia Cauti-Mendoza Norma Pérez-Martínez Kristel Gutierrez-Manchay Ingrid Ramirez-Ortiz Dennis Núñez-Fernández Mario I. Salguedo-Bohorquez Stefany Quiñones-Garcia Manolo Fernández Díaz Luis A. Guevara Sarmiento Mirko Zimic Mirko Zimic COVID-19 Working Group in Perú Andres Agurto-Arteaga Ricardo Antiparra Manuel Ardiles-Reyes Katherine Calderón Yudith Cauna-Orocollo Maria de Grecia Cauti-Mendoza Naer Chipana-Flores Ricardo Choque-Guevara Xiomara Chunga-Girón Manuel Criollo-Orozco Lewis De La Cruz Nicolás E. Delgado-Pease Elmer Delgado-Ccancce Christian Elugo-Guevara Manolo Fernández-Díaz Manolo Fernández-Sánchez Luis Guevara-Sarmiento Kristel Gutiérrez Oscar Heredia-Almeyda Edison Huaccachi-Gonzalez Pedro Huerta-Roque Eliana Icochea Gisela Isasi-Rivas Gabriel Jiménez-Avalos Romina A. Juscamaita-Bartra Abraham Licla-Inca Angela Montalván Ricardo Montesinos-Millán Dennis Núñez-Fernández Adiana Ochoa-Ortiz Gustavo E. Olivos-Ramirez Erika Páucar-Montoro Kathy Pauyac Jose L. Perez-Martinez Norma Pérez-M Astrid Poma-Acevedo Stefany Quiñones-Garcia Ingrid Ramirez-Ortiz Daniel Ramos-Sono Angela A. Rios-Angulo Dora Rios-Matos Aldo Rojas-Neyra Yomara K. Romero Mario I. Salguedo-Bohorquez Yacory Sernaque-Aguilar Patricia Sheen-Cortavarrı́a Luis F. Soto Luis Tataje-Lavanda Julio Ticona Katherine Vallejos-Sánchez A. Paula Vargas-Ruiz Doris Villanueva-Pérez Freddy Ygnacio-Aguirre Mirko Zimic-Peralta |
| author_facet | Andres Agurto-Arteaga Astrid Poma-Acevedo Dora Rios-Matos Ricardo Choque-Guevara Ricardo Montesinos-Millán Ángela Montalván Gisela Isasi-Rivas Yudith Cauna-Orocollo María de Grecia Cauti-Mendoza Norma Pérez-Martínez Kristel Gutierrez-Manchay Ingrid Ramirez-Ortiz Dennis Núñez-Fernández Mario I. Salguedo-Bohorquez Stefany Quiñones-Garcia Manolo Fernández Díaz Luis A. Guevara Sarmiento Mirko Zimic Mirko Zimic COVID-19 Working Group in Perú Andres Agurto-Arteaga Ricardo Antiparra Manuel Ardiles-Reyes Katherine Calderón Yudith Cauna-Orocollo Maria de Grecia Cauti-Mendoza Naer Chipana-Flores Ricardo Choque-Guevara Xiomara Chunga-Girón Manuel Criollo-Orozco Lewis De La Cruz Nicolás E. Delgado-Pease Elmer Delgado-Ccancce Christian Elugo-Guevara Manolo Fernández-Díaz Manolo Fernández-Sánchez Luis Guevara-Sarmiento Kristel Gutiérrez Oscar Heredia-Almeyda Edison Huaccachi-Gonzalez Pedro Huerta-Roque Eliana Icochea Gisela Isasi-Rivas Gabriel Jiménez-Avalos Romina A. Juscamaita-Bartra Abraham Licla-Inca Angela Montalván Ricardo Montesinos-Millán Dennis Núñez-Fernández Adiana Ochoa-Ortiz Gustavo E. Olivos-Ramirez Erika Páucar-Montoro Kathy Pauyac Jose L. Perez-Martinez Norma Pérez-M Astrid Poma-Acevedo Stefany Quiñones-Garcia Ingrid Ramirez-Ortiz Daniel Ramos-Sono Angela A. Rios-Angulo Dora Rios-Matos Aldo Rojas-Neyra Yomara K. Romero Mario I. Salguedo-Bohorquez Yacory Sernaque-Aguilar Patricia Sheen-Cortavarrı́a Luis F. Soto Luis Tataje-Lavanda Julio Ticona Katherine Vallejos-Sánchez A. Paula Vargas-Ruiz Doris Villanueva-Pérez Freddy Ygnacio-Aguirre Mirko Zimic-Peralta |
| author_sort | Andres Agurto-Arteaga |
| collection | DOAJ |
| description | Within the framework of the current COVID-19 pandemic, there is a race against time to find therapies for the outbreak to be controlled. Since vaccines are still tedious to develop and partially available for low-income countries, passive immunity based on egg-yolk antibodies (IgY) is presented as a suitable approach to preclude potential death of infected patients, based on its high specificity/avidity/production yield, cost-effective manufacture, and ease of administration. In the present study, IgY antibodies against a recombinant RBD protein of SARS-CoV-2 were produced in specific-pathogen-free chickens and purified from eggs using a biocompatible method. In vitro immunoreactivity was tested, finding high recognition and neutralization values. Safety was also demonstrated prior to efficacy evaluation, in which body weight, kinematics, and histopathological assessments of hamsters challenged with SARS-CoV-2 were performed, showing a protective effect administering IgY intranasally both as a prophylactic treatment or a post-infection treatment. The results of this study showed that intranasally delivered IgY has the potential to both aid in prevention and in overcoming COVID-19 infection, which should be very useful to control the advance of the current pandemic and the associated mortality. |
| first_indexed | 2024-12-12T03:38:22Z |
| format | Article |
| id | doaj.art-b87b2f7e669c4f6f8d6f5e8c60f85c40 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T03:38:22Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-b87b2f7e669c4f6f8d6f5e8c60f85c402022-12-22T00:39:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-05-011310.3389/fimmu.2022.881604881604Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19Andres Agurto-Arteaga0Astrid Poma-Acevedo1Dora Rios-Matos2Ricardo Choque-Guevara3Ricardo Montesinos-Millán4Ángela Montalván5Gisela Isasi-Rivas6Yudith Cauna-Orocollo7María de Grecia Cauti-Mendoza8Norma Pérez-Martínez9Kristel Gutierrez-Manchay10Ingrid Ramirez-Ortiz11Dennis Núñez-Fernández12Mario I. Salguedo-Bohorquez13Stefany Quiñones-Garcia14Manolo Fernández Díaz15Luis A. Guevara Sarmiento16Mirko Zimic17Mirko Zimic18COVID-19 Working Group in PerúAndres Agurto-ArteagaRicardo AntiparraManuel Ardiles-ReyesKatherine CalderónYudith Cauna-OrocolloMaria de Grecia Cauti-MendozaNaer Chipana-FloresRicardo Choque-GuevaraXiomara Chunga-GirónManuel Criollo-OrozcoLewis De La CruzNicolás E. Delgado-PeaseElmer Delgado-CcancceChristian Elugo-GuevaraManolo Fernández-DíazManolo Fernández-SánchezLuis Guevara-SarmientoKristel GutiérrezOscar Heredia-AlmeydaEdison Huaccachi-GonzalezPedro Huerta-RoqueEliana IcocheaGisela Isasi-RivasGabriel Jiménez-AvalosRomina A. Juscamaita-BartraAbraham Licla-IncaAngela MontalvánRicardo Montesinos-MillánDennis Núñez-FernándezAdiana Ochoa-OrtizGustavo E. Olivos-RamirezErika Páucar-MontoroKathy PauyacJose L. Perez-MartinezNorma Pérez-MAstrid Poma-AcevedoStefany Quiñones-GarciaIngrid Ramirez-OrtizDaniel Ramos-SonoAngela A. Rios-AnguloDora Rios-MatosAldo Rojas-NeyraYomara K. RomeroMario I. Salguedo-BohorquezYacory Sernaque-AguilarPatricia Sheen-Cortavarrı́aLuis F. SotoLuis Tataje-LavandaJulio TiconaKatherine Vallejos-SánchezA. Paula Vargas-RuizDoris Villanueva-PérezFreddy Ygnacio-AguirreMirko Zimic-PeraltaLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, PeruLaboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, PeruLaboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, PeruLaboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Biotecnología Molecular y Genómica, Laboratorios de Investigación y Desarrollo, Farmacológicos Veterinarios SAC (FARVET SAC), Chincha, PeruLaboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, PeruWithin the framework of the current COVID-19 pandemic, there is a race against time to find therapies for the outbreak to be controlled. Since vaccines are still tedious to develop and partially available for low-income countries, passive immunity based on egg-yolk antibodies (IgY) is presented as a suitable approach to preclude potential death of infected patients, based on its high specificity/avidity/production yield, cost-effective manufacture, and ease of administration. In the present study, IgY antibodies against a recombinant RBD protein of SARS-CoV-2 were produced in specific-pathogen-free chickens and purified from eggs using a biocompatible method. In vitro immunoreactivity was tested, finding high recognition and neutralization values. Safety was also demonstrated prior to efficacy evaluation, in which body weight, kinematics, and histopathological assessments of hamsters challenged with SARS-CoV-2 were performed, showing a protective effect administering IgY intranasally both as a prophylactic treatment or a post-infection treatment. The results of this study showed that intranasally delivered IgY has the potential to both aid in prevention and in overcoming COVID-19 infection, which should be very useful to control the advance of the current pandemic and the associated mortality.https://www.frontiersin.org/articles/10.3389/fimmu.2022.881604/fullSARS-CoV-2COVID-19egg-yolk antibodiesIgYpassive immunizationreceptor binding domain |
| spellingShingle | Andres Agurto-Arteaga Astrid Poma-Acevedo Dora Rios-Matos Ricardo Choque-Guevara Ricardo Montesinos-Millán Ángela Montalván Gisela Isasi-Rivas Yudith Cauna-Orocollo María de Grecia Cauti-Mendoza Norma Pérez-Martínez Kristel Gutierrez-Manchay Ingrid Ramirez-Ortiz Dennis Núñez-Fernández Mario I. Salguedo-Bohorquez Stefany Quiñones-Garcia Manolo Fernández Díaz Luis A. Guevara Sarmiento Mirko Zimic Mirko Zimic COVID-19 Working Group in Perú Andres Agurto-Arteaga Ricardo Antiparra Manuel Ardiles-Reyes Katherine Calderón Yudith Cauna-Orocollo Maria de Grecia Cauti-Mendoza Naer Chipana-Flores Ricardo Choque-Guevara Xiomara Chunga-Girón Manuel Criollo-Orozco Lewis De La Cruz Nicolás E. Delgado-Pease Elmer Delgado-Ccancce Christian Elugo-Guevara Manolo Fernández-Díaz Manolo Fernández-Sánchez Luis Guevara-Sarmiento Kristel Gutiérrez Oscar Heredia-Almeyda Edison Huaccachi-Gonzalez Pedro Huerta-Roque Eliana Icochea Gisela Isasi-Rivas Gabriel Jiménez-Avalos Romina A. Juscamaita-Bartra Abraham Licla-Inca Angela Montalván Ricardo Montesinos-Millán Dennis Núñez-Fernández Adiana Ochoa-Ortiz Gustavo E. Olivos-Ramirez Erika Páucar-Montoro Kathy Pauyac Jose L. Perez-Martinez Norma Pérez-M Astrid Poma-Acevedo Stefany Quiñones-Garcia Ingrid Ramirez-Ortiz Daniel Ramos-Sono Angela A. Rios-Angulo Dora Rios-Matos Aldo Rojas-Neyra Yomara K. Romero Mario I. Salguedo-Bohorquez Yacory Sernaque-Aguilar Patricia Sheen-Cortavarrı́a Luis F. Soto Luis Tataje-Lavanda Julio Ticona Katherine Vallejos-Sánchez A. Paula Vargas-Ruiz Doris Villanueva-Pérez Freddy Ygnacio-Aguirre Mirko Zimic-Peralta Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19 Frontiers in Immunology SARS-CoV-2 COVID-19 egg-yolk antibodies IgY passive immunization receptor binding domain |
| title | Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19 |
| title_full | Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19 |
| title_fullStr | Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19 |
| title_full_unstemmed | Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19 |
| title_short | Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19 |
| title_sort | preclinical assessment of igy antibodies against recombinant sars cov 2 rbd protein for prophylaxis and post infection treatment of covid 19 |
| topic | SARS-CoV-2 COVID-19 egg-yolk antibodies IgY passive immunization receptor binding domain |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.881604/full |
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