The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin Wounds
The treatment of full-thickness skin wounds is a problem in the clinical setting, as they do not heal spontaneously. Extensive pain at the donor site and a lack of skin grafts limit autogenic and allogeneic skin graft availability. We evaluated fetal bovine acellular dermal matrix (FADM) in combinat...
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MDPI AG
2023-04-01
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| author | Reyhaneh Nassiri Mansour Elham Hasanzadeh Mozhgan Abasi Mazaher Gholipourmalekabadi Amir Mellati Seyed Ehsan Enderami |
| author_facet | Reyhaneh Nassiri Mansour Elham Hasanzadeh Mozhgan Abasi Mazaher Gholipourmalekabadi Amir Mellati Seyed Ehsan Enderami |
| author_sort | Reyhaneh Nassiri Mansour |
| collection | DOAJ |
| description | The treatment of full-thickness skin wounds is a problem in the clinical setting, as they do not heal spontaneously. Extensive pain at the donor site and a lack of skin grafts limit autogenic and allogeneic skin graft availability. We evaluated fetal bovine acellular dermal matrix (FADM) in combination with human Wharton’s jelly mesenchymal stem cells (hWJ-MSCs) to heal full-thickness skin wounds. FADM was prepared from a 6-month-old trauma-aborted fetus. WJ-MSCs were derived from a human umbilical cord and seeded on the FADM. Rat models of full-thickness wounds were created and divided into three groups: control (no treatment), FADM, and FADM-WJMSCs groups. Wound treatment was evaluated microscopically and histologically on days 7, 14, and 21 post-surgery. The prepared FADM was porous and decellularized with a normal range of residual DNA. WJ-MSCs were seeded and proliferated on FADM effectively. The highest wound closure rate was observed in the FADM-WJMSC group on days 7 and 14 post-surgery. Furthermore, this group had fewer inflammatory cells than other groups. Finally, in this study, we observed that, without using the differential cell culture media of fibroblasts, the xenogeneic hWJSCs in combination with FADM could promote an increased rate of full-thickness skin wound closure with less inflammation. |
| first_indexed | 2024-03-11T04:59:20Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T04:59:20Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-b87d94c9eb8c4f8e8ebcdf034faaec442023-11-17T19:24:19ZengMDPI AGGenes2073-44252023-04-0114490910.3390/genes14040909The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin WoundsReyhaneh Nassiri Mansour0Elham Hasanzadeh1Mozhgan Abasi2Mazaher Gholipourmalekabadi3Amir Mellati4Seyed Ehsan Enderami5Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari 4815733971, IranDepartment of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari 4815733971, IranDepartment of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari 4815733971, IranCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran 1449614535, IranDepartment of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari 4815733971, IranImmunogenetics Research Center, Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari 4815733971, IranThe treatment of full-thickness skin wounds is a problem in the clinical setting, as they do not heal spontaneously. Extensive pain at the donor site and a lack of skin grafts limit autogenic and allogeneic skin graft availability. We evaluated fetal bovine acellular dermal matrix (FADM) in combination with human Wharton’s jelly mesenchymal stem cells (hWJ-MSCs) to heal full-thickness skin wounds. FADM was prepared from a 6-month-old trauma-aborted fetus. WJ-MSCs were derived from a human umbilical cord and seeded on the FADM. Rat models of full-thickness wounds were created and divided into three groups: control (no treatment), FADM, and FADM-WJMSCs groups. Wound treatment was evaluated microscopically and histologically on days 7, 14, and 21 post-surgery. The prepared FADM was porous and decellularized with a normal range of residual DNA. WJ-MSCs were seeded and proliferated on FADM effectively. The highest wound closure rate was observed in the FADM-WJMSC group on days 7 and 14 post-surgery. Furthermore, this group had fewer inflammatory cells than other groups. Finally, in this study, we observed that, without using the differential cell culture media of fibroblasts, the xenogeneic hWJSCs in combination with FADM could promote an increased rate of full-thickness skin wound closure with less inflammation.https://www.mdpi.com/2073-4425/14/4/909acellular dermal matrixWharton’s jelly mesenchymal stem cellsskin woundswound healingregenerative medicine |
| spellingShingle | Reyhaneh Nassiri Mansour Elham Hasanzadeh Mozhgan Abasi Mazaher Gholipourmalekabadi Amir Mellati Seyed Ehsan Enderami The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin Wounds Genes acellular dermal matrix Wharton’s jelly mesenchymal stem cells skin wounds wound healing regenerative medicine |
| title | The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin Wounds |
| title_full | The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin Wounds |
| title_fullStr | The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin Wounds |
| title_full_unstemmed | The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin Wounds |
| title_short | The Effect of Fetal Bovine Acellular Dermal Matrix Seeded with Wharton’s Jelly Mesenchymal Stem Cells for Healing Full-Thickness Skin Wounds |
| title_sort | effect of fetal bovine acellular dermal matrix seeded with wharton s jelly mesenchymal stem cells for healing full thickness skin wounds |
| topic | acellular dermal matrix Wharton’s jelly mesenchymal stem cells skin wounds wound healing regenerative medicine |
| url | https://www.mdpi.com/2073-4425/14/4/909 |
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