Oxidative Stress Response and Metal Transport in Roots of <i>Macleaya cordata</i> Exposed to Lead and Zinc
Heavy metal pollution possesses potential hazards to plant, animal and human health, which has become the focus of recent attention. Hence, phytoremediation has been regarded as one of the most important remediation technologies for heavy-metal-contaminated soils. In this research, a dominant mine t...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/3/516 |
| _version_ | 1797623488612139008 |
|---|---|
| author | Hongxiao Zhang Xijing Sun Delight Hwarari Xinlong Du Yinghao Wang Huawei Xu Shufang Lv Ting Wang Liming Yang Dianyun Hou |
| author_facet | Hongxiao Zhang Xijing Sun Delight Hwarari Xinlong Du Yinghao Wang Huawei Xu Shufang Lv Ting Wang Liming Yang Dianyun Hou |
| author_sort | Hongxiao Zhang |
| collection | DOAJ |
| description | Heavy metal pollution possesses potential hazards to plant, animal and human health, which has become the focus of recent attention. Hence, phytoremediation has been regarded as one of the most important remediation technologies for heavy-metal-contaminated soils. In this research, a dominant mine tailing plant, <i>Macleaya cordata</i>, was used as the experimental material to compare the metal transport and oxidative stress response in its roots under lead (Pb) and zinc (Zn) treatments. The result showed that Pb was mainly accumulated in the roots of <i>M. cordata</i> under the Pb treatment; less than 1% Pb was transported to the parts above. An analysis of the Zn content demonstrated a 39% accumulation in the shoots. The production of reactive oxygen species was detected using the in situ histological staining of roots, which showed that hydrogen peroxide in the root tips was observed to increase with the increase in both Pb and Zn concentrations. No significant superoxide anion changes were noted in the root tips under the Pb treatment. An analysis of the root enzyme activity showed that increase in NADPH oxidase activity can be responsible for the production of superoxide anions, subsequent the inhibition of root growth and decrease in antioxidant enzyme activities in the roots of <i>M. cordata</i> exposed to excess Zn. In total, this research provides evidence that the root of <i>M. cordata</i> has a high antioxidant capacity for Pb stress, so it can accumulate more Pb without oxidative damage. On the other hand, the Zn accumulated in the roots of <i>M. cordata</i> causes oxidative damage to the root tips, which can stimulate more Zn transport to the shoots to reduce the damage to the roots. This result will provide a basis for the application of <i>M. cordata</i> in the phytoremediation of soil polluted by Pb-Zn compounds. |
| first_indexed | 2024-03-11T09:29:42Z |
| format | Article |
| id | doaj.art-b87f9b1710ba4844881a1ee8d93dc2a5 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:29:42Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-b87f9b1710ba4844881a1ee8d93dc2a52023-11-16T17:43:32ZengMDPI AGPlants2223-77472023-01-0112351610.3390/plants12030516Oxidative Stress Response and Metal Transport in Roots of <i>Macleaya cordata</i> Exposed to Lead and ZincHongxiao Zhang0Xijing Sun1Delight Hwarari2Xinlong Du3Yinghao Wang4Huawei Xu5Shufang Lv6Ting Wang7Liming Yang8Dianyun Hou9College of Agriculture, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471023, ChinaHeavy metal pollution possesses potential hazards to plant, animal and human health, which has become the focus of recent attention. Hence, phytoremediation has been regarded as one of the most important remediation technologies for heavy-metal-contaminated soils. In this research, a dominant mine tailing plant, <i>Macleaya cordata</i>, was used as the experimental material to compare the metal transport and oxidative stress response in its roots under lead (Pb) and zinc (Zn) treatments. The result showed that Pb was mainly accumulated in the roots of <i>M. cordata</i> under the Pb treatment; less than 1% Pb was transported to the parts above. An analysis of the Zn content demonstrated a 39% accumulation in the shoots. The production of reactive oxygen species was detected using the in situ histological staining of roots, which showed that hydrogen peroxide in the root tips was observed to increase with the increase in both Pb and Zn concentrations. No significant superoxide anion changes were noted in the root tips under the Pb treatment. An analysis of the root enzyme activity showed that increase in NADPH oxidase activity can be responsible for the production of superoxide anions, subsequent the inhibition of root growth and decrease in antioxidant enzyme activities in the roots of <i>M. cordata</i> exposed to excess Zn. In total, this research provides evidence that the root of <i>M. cordata</i> has a high antioxidant capacity for Pb stress, so it can accumulate more Pb without oxidative damage. On the other hand, the Zn accumulated in the roots of <i>M. cordata</i> causes oxidative damage to the root tips, which can stimulate more Zn transport to the shoots to reduce the damage to the roots. This result will provide a basis for the application of <i>M. cordata</i> in the phytoremediation of soil polluted by Pb-Zn compounds.https://www.mdpi.com/2223-7747/12/3/516<i>Macleaya cordata</i>histochemical stainingrootsleadzincantioxidant |
| spellingShingle | Hongxiao Zhang Xijing Sun Delight Hwarari Xinlong Du Yinghao Wang Huawei Xu Shufang Lv Ting Wang Liming Yang Dianyun Hou Oxidative Stress Response and Metal Transport in Roots of <i>Macleaya cordata</i> Exposed to Lead and Zinc Plants <i>Macleaya cordata</i> histochemical staining roots lead zinc antioxidant |
| title | Oxidative Stress Response and Metal Transport in Roots of <i>Macleaya cordata</i> Exposed to Lead and Zinc |
| title_full | Oxidative Stress Response and Metal Transport in Roots of <i>Macleaya cordata</i> Exposed to Lead and Zinc |
| title_fullStr | Oxidative Stress Response and Metal Transport in Roots of <i>Macleaya cordata</i> Exposed to Lead and Zinc |
| title_full_unstemmed | Oxidative Stress Response and Metal Transport in Roots of <i>Macleaya cordata</i> Exposed to Lead and Zinc |
| title_short | Oxidative Stress Response and Metal Transport in Roots of <i>Macleaya cordata</i> Exposed to Lead and Zinc |
| title_sort | oxidative stress response and metal transport in roots of i macleaya cordata i exposed to lead and zinc |
| topic | <i>Macleaya cordata</i> histochemical staining roots lead zinc antioxidant |
| url | https://www.mdpi.com/2223-7747/12/3/516 |
| work_keys_str_mv | AT hongxiaozhang oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc AT xijingsun oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc AT delighthwarari oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc AT xinlongdu oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc AT yinghaowang oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc AT huaweixu oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc AT shufanglv oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc AT tingwang oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc AT limingyang oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc AT dianyunhou oxidativestressresponseandmetaltransportinrootsofimacleayacordataiexposedtoleadandzinc |