<i>Linum usitatissimum AccD</i> Enhances Seed Fatty Acid Accumulation and Tolerance to Environmental Stresses during Seed Germination in <i>Arabidopsis thaliana</i>
Flax (<i>Linum usitatissimum</i> L.), as an important oil-producing crop, is widely distributed throughout the world, and its seeds are rich in polyunsaturated fatty acids (FAs). Previous studies have revealed that <i>Arabidopsis thaliana</i> ACETYL-CoA CARBOXYLASE (AtACCase)...
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MDPI AG
2023-08-01
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author | Rui Du Xinye Li Huan Hu Yu Zhao Mingxun Chen Zijin Liu |
author_facet | Rui Du Xinye Li Huan Hu Yu Zhao Mingxun Chen Zijin Liu |
author_sort | Rui Du |
collection | DOAJ |
description | Flax (<i>Linum usitatissimum</i> L.), as an important oil-producing crop, is widely distributed throughout the world, and its seeds are rich in polyunsaturated fatty acids (FAs). Previous studies have revealed that <i>Arabidopsis thaliana</i> ACETYL-CoA CARBOXYLASE (AtACCase) is vital for FA biosynthesis. However, the functions of <i>L. usitatissimum AccD</i> (<i>LuAccD</i>) on FA accumulation and seed germination remain unclear. In the present study, we cloned the LuAccD coding sequence from the flax cultivar ‘Longya 10’, identified conserved protein domains, and performed a phylogenetic analysis to elucidate its relationship with homologs from a range of plant species. Ectopic expression of <i>LuAccD</i> in <i>A. thaliana</i> wild-type background enhanced seed FA accumulation without altering seed morphological characteristics, including seed size, 1000-seed weight, and seed coat color. Consistently, the expression of key genes involved in FA biosynthesis was greatly up-regulated in the developing seeds of <i>LuAccD</i> overexpression lines. Additionally, we demonstrated that LuAccD acts as a positive regulator of salt and mannitol tolerance during seed germination in <i>A. thaliana</i>. These results provide important insights into the functions of <i>LuAccD</i>, which facilitates the oil quantity and abiotic stress tolerance of oil-producing crops through genetic manipulation. |
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spelling | doaj.art-b87fd37d360a4f149c90881928fed8be2023-11-19T08:41:19ZengMDPI AGPlants2223-77472023-08-011217310010.3390/plants12173100<i>Linum usitatissimum AccD</i> Enhances Seed Fatty Acid Accumulation and Tolerance to Environmental Stresses during Seed Germination in <i>Arabidopsis thaliana</i>Rui Du0Xinye Li1Huan Hu2Yu Zhao3Mingxun Chen4Zijin Liu5National Yangling Agricultural Biotechnology & Breeding Center, Shaanxi Key Laboratory of Crop Heterosis and College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Shaanxi Key Laboratory of Crop Heterosis and College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Shaanxi Key Laboratory of Crop Heterosis and College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Shaanxi Key Laboratory of Crop Heterosis and College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Shaanxi Key Laboratory of Crop Heterosis and College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Shaanxi Key Laboratory of Crop Heterosis and College of Agronomy, Northwest A&F University, Yangling 712100, ChinaFlax (<i>Linum usitatissimum</i> L.), as an important oil-producing crop, is widely distributed throughout the world, and its seeds are rich in polyunsaturated fatty acids (FAs). Previous studies have revealed that <i>Arabidopsis thaliana</i> ACETYL-CoA CARBOXYLASE (AtACCase) is vital for FA biosynthesis. However, the functions of <i>L. usitatissimum AccD</i> (<i>LuAccD</i>) on FA accumulation and seed germination remain unclear. In the present study, we cloned the LuAccD coding sequence from the flax cultivar ‘Longya 10’, identified conserved protein domains, and performed a phylogenetic analysis to elucidate its relationship with homologs from a range of plant species. Ectopic expression of <i>LuAccD</i> in <i>A. thaliana</i> wild-type background enhanced seed FA accumulation without altering seed morphological characteristics, including seed size, 1000-seed weight, and seed coat color. Consistently, the expression of key genes involved in FA biosynthesis was greatly up-regulated in the developing seeds of <i>LuAccD</i> overexpression lines. Additionally, we demonstrated that LuAccD acts as a positive regulator of salt and mannitol tolerance during seed germination in <i>A. thaliana</i>. These results provide important insights into the functions of <i>LuAccD</i>, which facilitates the oil quantity and abiotic stress tolerance of oil-producing crops through genetic manipulation.https://www.mdpi.com/2223-7747/12/17/3100<i>LuAccD</i>fatty acidssalt stressmannitol stressseed germination |
spellingShingle | Rui Du Xinye Li Huan Hu Yu Zhao Mingxun Chen Zijin Liu <i>Linum usitatissimum AccD</i> Enhances Seed Fatty Acid Accumulation and Tolerance to Environmental Stresses during Seed Germination in <i>Arabidopsis thaliana</i> Plants <i>LuAccD</i> fatty acids salt stress mannitol stress seed germination |
title | <i>Linum usitatissimum AccD</i> Enhances Seed Fatty Acid Accumulation and Tolerance to Environmental Stresses during Seed Germination in <i>Arabidopsis thaliana</i> |
title_full | <i>Linum usitatissimum AccD</i> Enhances Seed Fatty Acid Accumulation and Tolerance to Environmental Stresses during Seed Germination in <i>Arabidopsis thaliana</i> |
title_fullStr | <i>Linum usitatissimum AccD</i> Enhances Seed Fatty Acid Accumulation and Tolerance to Environmental Stresses during Seed Germination in <i>Arabidopsis thaliana</i> |
title_full_unstemmed | <i>Linum usitatissimum AccD</i> Enhances Seed Fatty Acid Accumulation and Tolerance to Environmental Stresses during Seed Germination in <i>Arabidopsis thaliana</i> |
title_short | <i>Linum usitatissimum AccD</i> Enhances Seed Fatty Acid Accumulation and Tolerance to Environmental Stresses during Seed Germination in <i>Arabidopsis thaliana</i> |
title_sort | i linum usitatissimum accd i enhances seed fatty acid accumulation and tolerance to environmental stresses during seed germination in i arabidopsis thaliana i |
topic | <i>LuAccD</i> fatty acids salt stress mannitol stress seed germination |
url | https://www.mdpi.com/2223-7747/12/17/3100 |
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