Green synthesis and antibacterial activities of silver nanoparticles against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureus
Background: Over the last two decades infectious agents have become more dangerous, most especially in developing countries, due to their ability to develop resistance against orthodox medicines. Many in these countries are suffering from the debilitating effects of these pathogens without any remed...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Running Line
2017-02-01
|
| Series: | Advancements in Life Sciences |
| Subjects: | |
| Online Access: | http://www.als-journal.com/426-17/ |
| _version_ | 1797711260755689472 |
|---|---|
| author | Moses Enemaduku Abalaka Oghenerobor Benjamin Akpor Omorefosa O. Osemwegie |
| author_facet | Moses Enemaduku Abalaka Oghenerobor Benjamin Akpor Omorefosa O. Osemwegie |
| author_sort | Moses Enemaduku Abalaka |
| collection | DOAJ |
| description | Background: Over the last two decades infectious agents have become more dangerous, most especially in developing countries, due to their ability to develop resistance against orthodox medicines. Many in these countries are suffering from the debilitating effects of these pathogens without any remedies in sight. The recent researches in nanoparticles derived from medicinal plants seem to be yielding positive results.
Methods: We carried out synthesis of silver nanoparticles from AgNO3 and using Hyaluronic acid as a stabilizing agent to avoid aggregation in green synthesis from Ziziphus spinachristi and Garcinia kola. Transmission electron microscopy (TEM) was used to determine particle size and shape. Disc diffusion technique was used to study the susceptibility patterns of the particles on the test organisms- Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa and Staphylococcus aureus.
Result: The nanoparticles exhibited very high activity against the pathogens at very low concentration and showed remarkable higher activity than the crude extracts and standard antibiotics (control) with very wide zones of inhibition. The zones of inhibition ranged from 12.4±0.11 – 15.1±0.22 for the nanoparticles as against, 8.7±0.21 – 9.2±0.32 for the crude and 10.7±0.22 – 12.7±0.88 for the standard antibiotics.
Conclusion: Green synthesis of silver nanoparticles may give the long awaited breakthrough against these infectious agents to ameliorate, if not completely, win the war against these pathogens. |
| first_indexed | 2024-03-12T07:04:26Z |
| format | Article |
| id | doaj.art-b88425aa51594e3189c993a1190ffb16 |
| institution | Directory Open Access Journal |
| issn | 2310-5380 2310-5380 |
| language | English |
| last_indexed | 2024-03-12T07:04:26Z |
| publishDate | 2017-02-01 |
| publisher | The Running Line |
| record_format | Article |
| series | Advancements in Life Sciences |
| spelling | doaj.art-b88425aa51594e3189c993a1190ffb162023-09-02T23:32:54ZengThe Running LineAdvancements in Life Sciences2310-53802310-53802017-02-01426065Green synthesis and antibacterial activities of silver nanoparticles against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureusMoses Enemaduku Abalaka0Oghenerobor Benjamin Akpor1Omorefosa O. Osemwegie2Department of Microbiology, Federal University of Technology, Minna, Niger State – NigeriaDepartment of Biological sciences, Landmark University, Omu-Ara – NigeriaDepartment of Biological sciences, Landmark University, Omu-Ara – NigeriaBackground: Over the last two decades infectious agents have become more dangerous, most especially in developing countries, due to their ability to develop resistance against orthodox medicines. Many in these countries are suffering from the debilitating effects of these pathogens without any remedies in sight. The recent researches in nanoparticles derived from medicinal plants seem to be yielding positive results. Methods: We carried out synthesis of silver nanoparticles from AgNO3 and using Hyaluronic acid as a stabilizing agent to avoid aggregation in green synthesis from Ziziphus spinachristi and Garcinia kola. Transmission electron microscopy (TEM) was used to determine particle size and shape. Disc diffusion technique was used to study the susceptibility patterns of the particles on the test organisms- Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa and Staphylococcus aureus. Result: The nanoparticles exhibited very high activity against the pathogens at very low concentration and showed remarkable higher activity than the crude extracts and standard antibiotics (control) with very wide zones of inhibition. The zones of inhibition ranged from 12.4±0.11 – 15.1±0.22 for the nanoparticles as against, 8.7±0.21 – 9.2±0.32 for the crude and 10.7±0.22 – 12.7±0.88 for the standard antibiotics. Conclusion: Green synthesis of silver nanoparticles may give the long awaited breakthrough against these infectious agents to ameliorate, if not completely, win the war against these pathogens.http://www.als-journal.com/426-17/Green synthesisSilver nanoparticlesZiziphus spinachristiGarcinia kolaAntimicrobial activities |
| spellingShingle | Moses Enemaduku Abalaka Oghenerobor Benjamin Akpor Omorefosa O. Osemwegie Green synthesis and antibacterial activities of silver nanoparticles against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureus Advancements in Life Sciences Green synthesis Silver nanoparticles Ziziphus spinachristi Garcinia kola Antimicrobial activities |
| title | Green synthesis and antibacterial activities of silver nanoparticles against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureus |
| title_full | Green synthesis and antibacterial activities of silver nanoparticles against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureus |
| title_fullStr | Green synthesis and antibacterial activities of silver nanoparticles against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureus |
| title_full_unstemmed | Green synthesis and antibacterial activities of silver nanoparticles against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureus |
| title_short | Green synthesis and antibacterial activities of silver nanoparticles against Escherichia coli, Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureus |
| title_sort | green synthesis and antibacterial activities of silver nanoparticles against escherichia coli salmonella typhi pseudomonas aeruginosaand staphylococcus aureus |
| topic | Green synthesis Silver nanoparticles Ziziphus spinachristi Garcinia kola Antimicrobial activities |
| url | http://www.als-journal.com/426-17/ |
| work_keys_str_mv | AT mosesenemadukuabalaka greensynthesisandantibacterialactivitiesofsilvernanoparticlesagainstescherichiacolisalmonellatyphipseudomonasaeruginosaandstaphylococcusaureus AT ogheneroborbenjaminakpor greensynthesisandantibacterialactivitiesofsilvernanoparticlesagainstescherichiacolisalmonellatyphipseudomonasaeruginosaandstaphylococcusaureus AT omorefosaoosemwegie greensynthesisandantibacterialactivitiesofsilvernanoparticlesagainstescherichiacolisalmonellatyphipseudomonasaeruginosaandstaphylococcusaureus |