Ozone therapy improves early stages of osseointegration in ovariectomized rats
Abstract Objective the aim of this study was to analyze the influence of ozone therapy (OZN) on peri-implant bone repair in critical bones by installing osseointegrated implants in the tibia of ovariectomized rats. Methodology ovariectomy was performed on 30 Wistar rats, aged six months (Rattus...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2024-03-01
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572024000100414&lng=en&tlng=en |
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| author | William Phillip Pereira da SILVA João Matheus Fonseca e SANTOS Mônica Caroline de SOUZA Stéfany BARBOSA Anderson Maikon de Souza SANTOS Edilson ERVOLINO Ana Paula Farnezi BASSI Cortino SUKOTJO Leonardo P FAVERANI |
| author_facet | William Phillip Pereira da SILVA João Matheus Fonseca e SANTOS Mônica Caroline de SOUZA Stéfany BARBOSA Anderson Maikon de Souza SANTOS Edilson ERVOLINO Ana Paula Farnezi BASSI Cortino SUKOTJO Leonardo P FAVERANI |
| author_sort | William Phillip Pereira da SILVA |
| collection | DOAJ |
| description | Abstract Objective the aim of this study was to analyze the influence of ozone therapy (OZN) on peri-implant bone repair in critical bones by installing osseointegrated implants in the tibia of ovariectomized rats. Methodology ovariectomy was performed on 30 Wistar rats, aged six months (Rattus novergicus), and, after 90 days, osseointegrated implants were installed in each tibial metaphysis. The study groups were divided into the animals that received intraperitoneal ozone at a concentration of 700 mcg/kg — OZ Group (n=15) — and a control group that received an intraperitoneal saline solution and, for this reason, was named the SAL group (n=15). The applications for both groups occurred during the immediate post-operative period on the 2nd, 4th, 6th, 8th, 10th, and 12th day post-surgery. At various stages (14, 42, and 60 days), the animals were euthanized, and tests were performed on their tibiae. These tests include histomorphometric and immunohistochemical analyses, computerized microtomography, sampling in light-cured resin for calcified sections, and confocal microscopy. The obtained data were then analyzed using One-way ANOVA and the Shapiro-Wilk, Kruskal-Wallis, and student t-tests (P<0.05). Results our findings indicate that the OZ group (3.26±0.20 mm) showed better cellular organization and bone neoformation at 14 days (SAL group, 0.90±1.42 mm) (P=0.001). Immunohistochemistry revealed that osteocalcin labeling was moderate in the OZ group and mild in the SAL group at 14 and 42 days post-surgery. The data from the analysis of calcified tissues (microtomography, histometric, and bone dynamism analysis) at 60 days showed no statistically significant differences between the groups (P=0.32). Conclusion it was concluded that ozone therapy anticipated the initial phases of the peri-implant bone repair process. |
| first_indexed | 2024-04-24T19:15:32Z |
| format | Article |
| id | doaj.art-b884d92052074f04a8184350d0b3d753 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-04-24T19:15:32Z |
| publishDate | 2024-03-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-b884d92052074f04a8184350d0b3d7532024-03-26T07:40:59ZengUniversity of São PauloJournal of Applied Oral Science1678-77652024-03-013210.1590/1678-7757-2023-0172Ozone therapy improves early stages of osseointegration in ovariectomized ratsWilliam Phillip Pereira da SILVAJoão Matheus Fonseca e SANTOSMônica Caroline de SOUZAStéfany BARBOSAhttps://orcid.org/0000-0002-4190-7931Anderson Maikon de Souza SANTOSEdilson ERVOLINOhttps://orcid.org/0000-0003-4859-0583Ana Paula Farnezi BASSIhttps://orcid.org/0000-0002-0031-4953Cortino SUKOTJOhttps://orcid.org/0000-0002-2171-004XLeonardo P FAVERANIhttps://orcid.org/0000-0003-2249-3048Abstract Objective the aim of this study was to analyze the influence of ozone therapy (OZN) on peri-implant bone repair in critical bones by installing osseointegrated implants in the tibia of ovariectomized rats. Methodology ovariectomy was performed on 30 Wistar rats, aged six months (Rattus novergicus), and, after 90 days, osseointegrated implants were installed in each tibial metaphysis. The study groups were divided into the animals that received intraperitoneal ozone at a concentration of 700 mcg/kg — OZ Group (n=15) — and a control group that received an intraperitoneal saline solution and, for this reason, was named the SAL group (n=15). The applications for both groups occurred during the immediate post-operative period on the 2nd, 4th, 6th, 8th, 10th, and 12th day post-surgery. At various stages (14, 42, and 60 days), the animals were euthanized, and tests were performed on their tibiae. These tests include histomorphometric and immunohistochemical analyses, computerized microtomography, sampling in light-cured resin for calcified sections, and confocal microscopy. The obtained data were then analyzed using One-way ANOVA and the Shapiro-Wilk, Kruskal-Wallis, and student t-tests (P<0.05). Results our findings indicate that the OZ group (3.26±0.20 mm) showed better cellular organization and bone neoformation at 14 days (SAL group, 0.90±1.42 mm) (P=0.001). Immunohistochemistry revealed that osteocalcin labeling was moderate in the OZ group and mild in the SAL group at 14 and 42 days post-surgery. The data from the analysis of calcified tissues (microtomography, histometric, and bone dynamism analysis) at 60 days showed no statistically significant differences between the groups (P=0.32). Conclusion it was concluded that ozone therapy anticipated the initial phases of the peri-implant bone repair process.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572024000100414&lng=en&tlng=enOsteoporosisOzoneDental ImplantsDentistryPreclinical Drug Evaluation |
| spellingShingle | William Phillip Pereira da SILVA João Matheus Fonseca e SANTOS Mônica Caroline de SOUZA Stéfany BARBOSA Anderson Maikon de Souza SANTOS Edilson ERVOLINO Ana Paula Farnezi BASSI Cortino SUKOTJO Leonardo P FAVERANI Ozone therapy improves early stages of osseointegration in ovariectomized rats Journal of Applied Oral Science Osteoporosis Ozone Dental Implants Dentistry Preclinical Drug Evaluation |
| title | Ozone therapy improves early stages of osseointegration in ovariectomized rats |
| title_full | Ozone therapy improves early stages of osseointegration in ovariectomized rats |
| title_fullStr | Ozone therapy improves early stages of osseointegration in ovariectomized rats |
| title_full_unstemmed | Ozone therapy improves early stages of osseointegration in ovariectomized rats |
| title_short | Ozone therapy improves early stages of osseointegration in ovariectomized rats |
| title_sort | ozone therapy improves early stages of osseointegration in ovariectomized rats |
| topic | Osteoporosis Ozone Dental Implants Dentistry Preclinical Drug Evaluation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572024000100414&lng=en&tlng=en |
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