Identification of virulence properties in Salmonella Typhimurium DT104 using Caenorhabditis elegans.
Salmonella enterica serover Typhimurium definitive phage type DT104, resistant to multiple antibiotics, is one of the most widespread Salmonella species in human infection worldwide. Although several cohort studies indicate that DT104 carrying the multidrug resistance (MDR) locus on salmonella genom...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3790755?pdf=render |
| _version_ | 1819089562810449920 |
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| author | Surasri N Sahu Yuda Anriany Christopher J Grim Sungji Kim Zenas Chang Sam W Joseph Hediye N Cinar |
| author_facet | Surasri N Sahu Yuda Anriany Christopher J Grim Sungji Kim Zenas Chang Sam W Joseph Hediye N Cinar |
| author_sort | Surasri N Sahu |
| collection | DOAJ |
| description | Salmonella enterica serover Typhimurium definitive phage type DT104, resistant to multiple antibiotics, is one of the most widespread Salmonella species in human infection worldwide. Although several cohort studies indicate that DT104 carrying the multidrug resistance (MDR) locus on salmonella genomic island 1 is a possible hyper-virulent strain compared to DT104 strains without MDR, or other Salmonella enterica serotypes, existing experimental evidence regarding virulence properties associated with the MDR region is controversial. To address this question, we constructed an isogenic MDR deletion (∆MDR) mutant strain of DT104, SNS12, by allelic exchange and used Caenorhabditis elegans as a host model to assess differences in virulence between these two strains. SNS12 exhibited decreased virulence in C. elegans, and we observed increased colonization and proliferation of the intestine of C. elegans by DT104. The immune response against MDR-carrying DT104 appears to function through a non-canonical Unfolded Protein Response (UPR) pathway, namely prion-like-(QN-rich)-domain-bearing protein pathway (PQN), in a ced-1 dependent manner in C. elegans. Further, we also demonstrate that genes of the PQN pathway and antimicrobial peptide gene abf-2, are expressed at higher transcriptional levels in worms immediately following exposure to DT104, in comparison with worms exposed to SNS12. Altogether, our results suggest that the MDR region of Salmonella Typhimurium DT104 has a direct role in virulence against Caenorhabditis elegans. |
| first_indexed | 2024-12-21T22:09:55Z |
| format | Article |
| id | doaj.art-b885cc19b5e3494b8f55dd26b3d426e7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T22:09:55Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b885cc19b5e3494b8f55dd26b3d426e72022-12-21T18:48:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7667310.1371/journal.pone.0076673Identification of virulence properties in Salmonella Typhimurium DT104 using Caenorhabditis elegans.Surasri N SahuYuda AnrianyChristopher J GrimSungji KimZenas ChangSam W JosephHediye N CinarSalmonella enterica serover Typhimurium definitive phage type DT104, resistant to multiple antibiotics, is one of the most widespread Salmonella species in human infection worldwide. Although several cohort studies indicate that DT104 carrying the multidrug resistance (MDR) locus on salmonella genomic island 1 is a possible hyper-virulent strain compared to DT104 strains without MDR, or other Salmonella enterica serotypes, existing experimental evidence regarding virulence properties associated with the MDR region is controversial. To address this question, we constructed an isogenic MDR deletion (∆MDR) mutant strain of DT104, SNS12, by allelic exchange and used Caenorhabditis elegans as a host model to assess differences in virulence between these two strains. SNS12 exhibited decreased virulence in C. elegans, and we observed increased colonization and proliferation of the intestine of C. elegans by DT104. The immune response against MDR-carrying DT104 appears to function through a non-canonical Unfolded Protein Response (UPR) pathway, namely prion-like-(QN-rich)-domain-bearing protein pathway (PQN), in a ced-1 dependent manner in C. elegans. Further, we also demonstrate that genes of the PQN pathway and antimicrobial peptide gene abf-2, are expressed at higher transcriptional levels in worms immediately following exposure to DT104, in comparison with worms exposed to SNS12. Altogether, our results suggest that the MDR region of Salmonella Typhimurium DT104 has a direct role in virulence against Caenorhabditis elegans.http://europepmc.org/articles/PMC3790755?pdf=render |
| spellingShingle | Surasri N Sahu Yuda Anriany Christopher J Grim Sungji Kim Zenas Chang Sam W Joseph Hediye N Cinar Identification of virulence properties in Salmonella Typhimurium DT104 using Caenorhabditis elegans. PLoS ONE |
| title | Identification of virulence properties in Salmonella Typhimurium DT104 using Caenorhabditis elegans. |
| title_full | Identification of virulence properties in Salmonella Typhimurium DT104 using Caenorhabditis elegans. |
| title_fullStr | Identification of virulence properties in Salmonella Typhimurium DT104 using Caenorhabditis elegans. |
| title_full_unstemmed | Identification of virulence properties in Salmonella Typhimurium DT104 using Caenorhabditis elegans. |
| title_short | Identification of virulence properties in Salmonella Typhimurium DT104 using Caenorhabditis elegans. |
| title_sort | identification of virulence properties in salmonella typhimurium dt104 using caenorhabditis elegans |
| url | http://europepmc.org/articles/PMC3790755?pdf=render |
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