Multilocation proteins in organelle communication: Based on protein–protein interactions
Abstract Protein–protein interaction (PPI) plays a crucial role in most biological processes, including signal transduction and cell apoptosis. Importantly, the knowledge of PPIs can be useful for identification of multimeric protein complexes and elucidation of uncharacterized protein functions. Ar...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-02-01
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| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.386 |
| _version_ | 1819279281562320896 |
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| author | Erhui Xiong Di Cao Chengxin Qu Pengfei Zhao Zhaokun Wu Dongmei Yin Quanzhi Zhao Fangping Gong |
| author_facet | Erhui Xiong Di Cao Chengxin Qu Pengfei Zhao Zhaokun Wu Dongmei Yin Quanzhi Zhao Fangping Gong |
| author_sort | Erhui Xiong |
| collection | DOAJ |
| description | Abstract Protein–protein interaction (PPI) plays a crucial role in most biological processes, including signal transduction and cell apoptosis. Importantly, the knowledge of PPIs can be useful for identification of multimeric protein complexes and elucidation of uncharacterized protein functions. Arabidopsis thaliana, the best‐characterized dicotyledonous plant, the steadily increasing amount of information on the levels of its proteome and signaling pathways is progressively enabling more researchers to construct models for cellular processes for the plant, which in turn encourages more experimental data to be generated. In this study, we performed an overview analysis of the 10 major organelles and their associated proteins of the dicotyledonous model plant Arabidopsis thaliana via PPI network, and found that PPI may play an important role in organelle communication. Further, multilocation proteins, especially phosphorylation‐related multilocation proteins, can function as a “needle and thread” via PPIs and play an important role in organelle communication. Similar results were obtained in a monocotyledonous model crop, rice. Furthermore, we provide a research strategy for multilocation proteins by LOPIT technique, proteomics, and bioinformatics analysis and also describe their potential role in the field of plant science. The results provide a new view that the phosphorylation‐related multilocation proteins play an important role in organelle communication and provide new insight into PPIs and novel directions for proteomic research. The research of phosphorylation‐related multilocation proteins may promote the development of organelle communication and provide an important theoretical basis for plant responses to external stress. |
| first_indexed | 2024-12-24T00:25:25Z |
| format | Article |
| id | doaj.art-b88bdcc9980c4532a9e94a4e1de62007 |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-12-24T00:25:25Z |
| publishDate | 2022-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-b88bdcc9980c4532a9e94a4e1de620072022-12-21T17:24:27ZengWileyPlant Direct2475-44552022-02-0162n/an/a10.1002/pld3.386Multilocation proteins in organelle communication: Based on protein–protein interactionsErhui Xiong0Di Cao1Chengxin Qu2Pengfei Zhao3Zhaokun Wu4Dongmei Yin5Quanzhi Zhao6Fangping Gong7College of Agronomy Henan Agricultural University Zhengzhou ChinaCollege of Agronomy Henan Agricultural University Zhengzhou ChinaCollege of Agronomy Henan Agricultural University Zhengzhou ChinaCollege of Agronomy Henan Agricultural University Zhengzhou ChinaCollege of Agronomy Henan Agricultural University Zhengzhou ChinaCollege of Agronomy Henan Agricultural University Zhengzhou ChinaCollege of Agronomy Henan Agricultural University Zhengzhou ChinaCollege of Agronomy Henan Agricultural University Zhengzhou ChinaAbstract Protein–protein interaction (PPI) plays a crucial role in most biological processes, including signal transduction and cell apoptosis. Importantly, the knowledge of PPIs can be useful for identification of multimeric protein complexes and elucidation of uncharacterized protein functions. Arabidopsis thaliana, the best‐characterized dicotyledonous plant, the steadily increasing amount of information on the levels of its proteome and signaling pathways is progressively enabling more researchers to construct models for cellular processes for the plant, which in turn encourages more experimental data to be generated. In this study, we performed an overview analysis of the 10 major organelles and their associated proteins of the dicotyledonous model plant Arabidopsis thaliana via PPI network, and found that PPI may play an important role in organelle communication. Further, multilocation proteins, especially phosphorylation‐related multilocation proteins, can function as a “needle and thread” via PPIs and play an important role in organelle communication. Similar results were obtained in a monocotyledonous model crop, rice. Furthermore, we provide a research strategy for multilocation proteins by LOPIT technique, proteomics, and bioinformatics analysis and also describe their potential role in the field of plant science. The results provide a new view that the phosphorylation‐related multilocation proteins play an important role in organelle communication and provide new insight into PPIs and novel directions for proteomic research. The research of phosphorylation‐related multilocation proteins may promote the development of organelle communication and provide an important theoretical basis for plant responses to external stress.https://doi.org/10.1002/pld3.386multilocation proteinorganelle separationorganelles communicationphosphorylationprotein interaction |
| spellingShingle | Erhui Xiong Di Cao Chengxin Qu Pengfei Zhao Zhaokun Wu Dongmei Yin Quanzhi Zhao Fangping Gong Multilocation proteins in organelle communication: Based on protein–protein interactions Plant Direct multilocation protein organelle separation organelles communication phosphorylation protein interaction |
| title | Multilocation proteins in organelle communication: Based on protein–protein interactions |
| title_full | Multilocation proteins in organelle communication: Based on protein–protein interactions |
| title_fullStr | Multilocation proteins in organelle communication: Based on protein–protein interactions |
| title_full_unstemmed | Multilocation proteins in organelle communication: Based on protein–protein interactions |
| title_short | Multilocation proteins in organelle communication: Based on protein–protein interactions |
| title_sort | multilocation proteins in organelle communication based on protein protein interactions |
| topic | multilocation protein organelle separation organelles communication phosphorylation protein interaction |
| url | https://doi.org/10.1002/pld3.386 |
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