Molecular Cloning and Expression Profile of Class <i>E</i> Genes Related to Sepal Development in <i>Nelumbo nucifera</i>
The lotus (<i>Nelumbo</i> Adans.) is an important aquatic plant with ornamental, medicinal and edible values and cultural connotations. It has single-, semi-double-, double- and thousand-petalled types of flower shape and is an ideal material for developmental research of flower doubling...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2223-7747/10/8/1629 |
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| author | Zhuoxing Liu Dasheng Zhang Weiwei Zhang Lei Xiong Qingqing Liu Fengluan Liu Hanchun Li Xiangjie An Lijie Cui Daike Tian |
| author_facet | Zhuoxing Liu Dasheng Zhang Weiwei Zhang Lei Xiong Qingqing Liu Fengluan Liu Hanchun Li Xiangjie An Lijie Cui Daike Tian |
| author_sort | Zhuoxing Liu |
| collection | DOAJ |
| description | The lotus (<i>Nelumbo</i> Adans.) is an important aquatic plant with ornamental, medicinal and edible values and cultural connotations. It has single-, semi-double-, double- and thousand-petalled types of flower shape and is an ideal material for developmental research of flower doubling. The lotus is a basal eudicot species without a morphological difference between the sepals and petals and occupies a critical phylogenetic position in flowering plants. In order to investigate the genetic relationship between the sepals and petals in the lotus, the class <i>E</i> genes which affect sepal formation were focused on and analyzed. Here, <i>SEPALLATA 1</i>(<i>NnSEP1</i>) and its homologous genes <i>AGAMOUS-LIKE MADS-BOXAGL9</i> (<i>NnAGL9</i>) and <i>MADS-BOX TRANSCRIPTION FACTOR 6-like</i> (<i>NnMADS6-like</i>) of the class <i>E</i> gene family were isolated from the flower buds of the Asian lotus (<i>Nelumbo nucifera</i> Gaertn.). The protein structure, subcellular localization and expression patterns of these three genes were investigated. All three genes were verified to locate in the nucleus and had typical MADS-box characteristics. <i>NnSEP1</i> and <i>NnMADS6-like</i> were specifically expressed in the sepals, while <i>NnAGL9</i> was highly expressed in the petals, suggesting that different developmental mechanisms exist in the formation of the sepals and petals in the lotus. The significant functional differences between NnSEP1, NnMADS6-like and NnAGL9 were also confirmed by a yeast two-hybrid assay. These results expand our knowledge on the class <i>E</i> gene family in sepal formation and will benefit fundamental research on the development of floral organs in <i>Nelumbo.</i> |
| first_indexed | 2024-03-10T08:27:37Z |
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| id | doaj.art-b88cd3ecc0444cdd92c9be4d05b71e40 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T08:27:37Z |
| publishDate | 2021-08-01 |
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| series | Plants |
| spelling | doaj.art-b88cd3ecc0444cdd92c9be4d05b71e402023-11-22T09:19:12ZengMDPI AGPlants2223-77472021-08-01108162910.3390/plants10081629Molecular Cloning and Expression Profile of Class <i>E</i> Genes Related to Sepal Development in <i>Nelumbo nucifera</i>Zhuoxing Liu0Dasheng Zhang1Weiwei Zhang2Lei Xiong3Qingqing Liu4Fengluan Liu5Hanchun Li6Xiangjie An7Lijie Cui8Daike Tian9Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center of Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center of Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaDepartment of Plant Science and Technology, Shanghai Vocational College of Agriculture and Forestry, Shanghai 201699, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center of Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center of Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaDevelopment Center of Plant Germplam Resources, College of Life Science, Shanghai Normal University, Shanghai 200234, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center of Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center of Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaDevelopment Center of Plant Germplam Resources, College of Life Science, Shanghai Normal University, Shanghai 200234, ChinaShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center of Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, Shanghai 201602, ChinaThe lotus (<i>Nelumbo</i> Adans.) is an important aquatic plant with ornamental, medicinal and edible values and cultural connotations. It has single-, semi-double-, double- and thousand-petalled types of flower shape and is an ideal material for developmental research of flower doubling. The lotus is a basal eudicot species without a morphological difference between the sepals and petals and occupies a critical phylogenetic position in flowering plants. In order to investigate the genetic relationship between the sepals and petals in the lotus, the class <i>E</i> genes which affect sepal formation were focused on and analyzed. Here, <i>SEPALLATA 1</i>(<i>NnSEP1</i>) and its homologous genes <i>AGAMOUS-LIKE MADS-BOXAGL9</i> (<i>NnAGL9</i>) and <i>MADS-BOX TRANSCRIPTION FACTOR 6-like</i> (<i>NnMADS6-like</i>) of the class <i>E</i> gene family were isolated from the flower buds of the Asian lotus (<i>Nelumbo nucifera</i> Gaertn.). The protein structure, subcellular localization and expression patterns of these three genes were investigated. All three genes were verified to locate in the nucleus and had typical MADS-box characteristics. <i>NnSEP1</i> and <i>NnMADS6-like</i> were specifically expressed in the sepals, while <i>NnAGL9</i> was highly expressed in the petals, suggesting that different developmental mechanisms exist in the formation of the sepals and petals in the lotus. The significant functional differences between NnSEP1, NnMADS6-like and NnAGL9 were also confirmed by a yeast two-hybrid assay. These results expand our knowledge on the class <i>E</i> gene family in sepal formation and will benefit fundamental research on the development of floral organs in <i>Nelumbo.</i>https://www.mdpi.com/2223-7747/10/8/1629MADS-box<i>SEPALLATA</i><i>AGL9</i><i>MADS6-like</i>sepalpetal |
| spellingShingle | Zhuoxing Liu Dasheng Zhang Weiwei Zhang Lei Xiong Qingqing Liu Fengluan Liu Hanchun Li Xiangjie An Lijie Cui Daike Tian Molecular Cloning and Expression Profile of Class <i>E</i> Genes Related to Sepal Development in <i>Nelumbo nucifera</i> Plants MADS-box <i>SEPALLATA</i> <i>AGL9</i> <i>MADS6-like</i> sepal petal |
| title | Molecular Cloning and Expression Profile of Class <i>E</i> Genes Related to Sepal Development in <i>Nelumbo nucifera</i> |
| title_full | Molecular Cloning and Expression Profile of Class <i>E</i> Genes Related to Sepal Development in <i>Nelumbo nucifera</i> |
| title_fullStr | Molecular Cloning and Expression Profile of Class <i>E</i> Genes Related to Sepal Development in <i>Nelumbo nucifera</i> |
| title_full_unstemmed | Molecular Cloning and Expression Profile of Class <i>E</i> Genes Related to Sepal Development in <i>Nelumbo nucifera</i> |
| title_short | Molecular Cloning and Expression Profile of Class <i>E</i> Genes Related to Sepal Development in <i>Nelumbo nucifera</i> |
| title_sort | molecular cloning and expression profile of class i e i genes related to sepal development in i nelumbo nucifera i |
| topic | MADS-box <i>SEPALLATA</i> <i>AGL9</i> <i>MADS6-like</i> sepal petal |
| url | https://www.mdpi.com/2223-7747/10/8/1629 |
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