P17

The most effective method in treatment of inoperable cancer is currently miniallogeneic transplantation. This type of treatment focuses on reaction of “graft-versus-tumor”. According to preliminary data, such treatment leads to long-term remission in patients with metastatic cancer, who did not resp...

Full description

Bibliographic Details
Main Authors: O. Kokorev, V. Hodorenko, V. Gunther
Format: Article
Language:English
Published: Elsevier 2015-11-01
Series:EJC Supplements
Online Access:http://www.sciencedirect.com/science/article/pii/S1359634915000464
Description
Summary:The most effective method in treatment of inoperable cancer is currently miniallogeneic transplantation. This type of treatment focuses on reaction of “graft-versus-tumor”. According to preliminary data, such treatment leads to long-term remission in patients with metastatic cancer, who did not respond to previous therapy. Anti-tumor immune response after transplant may be extended or enhanced by additional infusion of donor lymphocytes. Research has shown that introduction of donor lymphocytes achieves complete remission of the tumor, even in case of relapse after allogeneic transplantation from the same donor, but these effects are unstable and not so long because of rapid elimination of donor lymphocytes [1]. The main problem is the preservation of cell transplantation viability and functional activity of the cells in patient’s body, and protecting them from recipient’s immune system. In recent years, most suitable for this purpose are the three-dimensional porous biomaterials (3D-scaffold). Specific pore space does not allow immunity effectors quickly destroy the cells in scaffold [2,3]. Research Institute of medical materials and implants with shape memory at the Tomsk State University created a porous-permeable incubators (scaffold) of TiNi-based shape memory alloy (TiNi). The material has unique properties: permeable porous structure by open interconnected pores, characterized by high degree of wettability with tissue media and nanoporous inner surface of the pore walls, exhibits high adhesion to various cell types, so all mentioned meet requirements of the bio-chemical and bio-mechanical compatibility. Porous TiNi-based scaffolds allow continually to keep the functionality cells and prolong their action [4,5]. The purpose of this study was to investigate possibility of modulation of antitumor response in allogeneic bone marrow transplantation in porous-permeable incubator of TiNi. Intraperitoneal injection of allogeneic bone marrow (BM) reduces metastasis by 30% and has easy 10%-antitumor effect. At the same time, the implantation of bone marrow cells on incubator of TiNi (BM + TiNi) leads to more pronounced antitumor (25%) and significant antimetastatic effects (45%). Life span of animals with tumors and implanted bone marrow cells on incubator TiNi is increased by 60% Since bone marrow cells do not have direct antiproliferative effect on tumor target cells in vitro, it is assumed that one of the mechanisms effecting the bone marrow transplantation on neoplastic process is the stimulation of endogenous effectors of antitumor immunity. The study of morphological parameters immunocompetent organs showed that administration of allogeneic bone marrow cells can reduce thymic regression, decrease splenomegaly at animals with transplanted tumors. Conclusion: Allogeneic transplantation of bone marrow cells on porous incubator was shown to prolong and enhance antitumor and antimetastatic action compared to injecting the cells. This effect is directly due to the stimulation of antitumor immunity, that is described by the study of immunity factors. The results show perspectives of porous TiNi-based scaffolds in complex treatment of neoplastic diseases.
ISSN:1359-6349