Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry[S]
Previous studies have shown that MALDI-imaging mass spectrometry (IMS) can be used to visualize the distribution of various biomolecules, especially lipids, in the cells and tissues. In this study, we report the cell-selective distribution of PUFA-containing glycerophospholipids (GPLs) in the mouse...
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| Format: | Article |
| Language: | English |
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Elsevier
2009-09-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520307306 |
| _version_ | 1818732538427867136 |
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| author | Yuki Sugiura Yoshiyuki Konishi Nobuhiro Zaima Shigeki Kajihara Hiroki Nakanishi Ryo Taguchi Mitsutoshi Setou |
| author_facet | Yuki Sugiura Yoshiyuki Konishi Nobuhiro Zaima Shigeki Kajihara Hiroki Nakanishi Ryo Taguchi Mitsutoshi Setou |
| author_sort | Yuki Sugiura |
| collection | DOAJ |
| description | Previous studies have shown that MALDI-imaging mass spectrometry (IMS) can be used to visualize the distribution of various biomolecules, especially lipids, in the cells and tissues. In this study, we report the cell-selective distribution of PUFA-containing glycerophospholipids (GPLs) in the mouse brain. We established a practical experimental procedure for the IMS of GPLs. We demonstrated that optimization of the composition of the matrix solution and spectrum normalization to the total ion current (TIC) is critical. Using our procedure, we simultaneously differentiated and visualized the localizations of specific molecular species of GPLs in mouse brain sections. The results showed that PUFA-containing phosphatidylcholines (PCs) were distributed in a cell-selective manner: arachidonic acid- and docosahexaenoic acid-containing PCs were seen in the hippocampal neurons and cerebellar Purkinje cells, respectively. Furthermore, these characteristic localizations of PUFA-PCs were formed during neuronal maturation. The phenomenon of brain cell-selective production of specific PUFA-GPLs will help elucidate the potential physiological functions of PUFAs in specific brain regions. |
| first_indexed | 2024-12-17T23:35:10Z |
| format | Article |
| id | doaj.art-b89357ce483049c2acb8d19c79c5a87a |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T23:35:10Z |
| publishDate | 2009-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-b89357ce483049c2acb8d19c79c5a87a2022-12-21T21:28:35ZengElsevierJournal of Lipid Research0022-22752009-09-0150917761788Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry[S]Yuki Sugiura0Yoshiyuki Konishi1Nobuhiro Zaima2Shigeki Kajihara3Hiroki Nakanishi4Ryo Taguchi5Mitsutoshi Setou6Department of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan; Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan; Department of Molecular Anatomy, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, JapanMitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan; Department of Molecular Anatomy, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, JapanMitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan; Department of Molecular Anatomy, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, JapanTechnology Research Laboratory, Shimadzu Corporation, 3-9-4, Hikaridai, Seika-cho, Souraku-gun, Kyoto 619-0237, JapanDepartment of Metabolome, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongou, Bunkyo-ku, Tokyo 113-0033, Japan; Core Research for Evolutional Science and Technology, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, JapanDepartment of Metabolome, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongou, Bunkyo-ku, Tokyo 113-0033, Japan; Core Research for Evolutional Science and Technology, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, JapanTo whom correspondence should be addressed.; Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan; Department of Molecular Anatomy, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, JapanPrevious studies have shown that MALDI-imaging mass spectrometry (IMS) can be used to visualize the distribution of various biomolecules, especially lipids, in the cells and tissues. In this study, we report the cell-selective distribution of PUFA-containing glycerophospholipids (GPLs) in the mouse brain. We established a practical experimental procedure for the IMS of GPLs. We demonstrated that optimization of the composition of the matrix solution and spectrum normalization to the total ion current (TIC) is critical. Using our procedure, we simultaneously differentiated and visualized the localizations of specific molecular species of GPLs in mouse brain sections. The results showed that PUFA-containing phosphatidylcholines (PCs) were distributed in a cell-selective manner: arachidonic acid- and docosahexaenoic acid-containing PCs were seen in the hippocampal neurons and cerebellar Purkinje cells, respectively. Furthermore, these characteristic localizations of PUFA-PCs were formed during neuronal maturation. The phenomenon of brain cell-selective production of specific PUFA-GPLs will help elucidate the potential physiological functions of PUFAs in specific brain regions.http://www.sciencedirect.com/science/article/pii/S0022227520307306MALDIimaging MSLC ESI-MS/MSphospholipidsdevelopment |
| spellingShingle | Yuki Sugiura Yoshiyuki Konishi Nobuhiro Zaima Shigeki Kajihara Hiroki Nakanishi Ryo Taguchi Mitsutoshi Setou Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry[S] Journal of Lipid Research MALDI imaging MS LC ESI-MS/MS phospholipids development |
| title | Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry[S] |
| title_full | Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry[S] |
| title_fullStr | Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry[S] |
| title_full_unstemmed | Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry[S] |
| title_short | Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry[S] |
| title_sort | visualization of the cell selective distribution of pufa containing phosphatidylcholines in mouse brain by imaging mass spectrometry s |
| topic | MALDI imaging MS LC ESI-MS/MS phospholipids development |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520307306 |
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