A Molecular Marker to Identify <i>Spodoptera frugiperda</i> (JE Smith) DNA in Predators’ Gut Content
<i>Spodoptera frugiperda</i> is a serious pest of maize and other crops worldwide. The integration of control tactics is recommended for <i>S. frugiperda</i> suppression because reports of insecticide and Btplant-resistance are frequent. Biological control agents would be an...
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| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/7/635 |
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| author | Daniela Hipolito Maggio Victória Zannuzzi Rossetti Larissa Muniz Amaral Santos Felipe Levorato Carmezini Alberto Soares Corrêa |
| author_facet | Daniela Hipolito Maggio Victória Zannuzzi Rossetti Larissa Muniz Amaral Santos Felipe Levorato Carmezini Alberto Soares Corrêa |
| author_sort | Daniela Hipolito Maggio |
| collection | DOAJ |
| description | <i>Spodoptera frugiperda</i> is a serious pest of maize and other crops worldwide. The integration of control tactics is recommended for <i>S. frugiperda</i> suppression because reports of insecticide and Btplant-resistance are frequent. Biological control agents would be an alternative to improve <i>S. frugiperda</i> control in agricultural areas. We constructed a species-specific molecular marker to detect <i>S. frugiperda</i> DNA in predators’ gut content and estimated the predation rates of ladybugs and earwigs on <i>S. frugiperda</i> in maize crops. Predators were sampled in Pirassununga, São Paulo state, Brazil, in 2020 and 2021. Using the species-specific molecular marker in laboratory conditions, we estimated the half-life time to detect <i>S. frugiperda</i> DNA in the gut contents of <i>Hippodamia convergens</i> as 6.16 h and <i>Doru luteipes</i> as 25.72 h. The weekly predation rate of <i>S. frugiperda</i> by predators in maize crop varied from 0 to 42.1% by ladybugs and from 0 to 9.2% by <i>D. luteipes</i>. Predation events on <i>S. frugiperda</i> by predators were more frequent during the maize reproductive stage. Our results confirmed that predators might contribute to <i>S. frugiperda</i> suppression in maize fields. However, further studies of prey–predator interactions and agricultural landscapes are essential for a better understanding of predator dynamics in crops. |
| first_indexed | 2024-03-09T03:20:45Z |
| format | Article |
| id | doaj.art-b894cdd1cd254d45af5eeeaacb2666b7 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T03:20:45Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-b894cdd1cd254d45af5eeeaacb2666b72023-12-03T15:11:53ZengMDPI AGInsects2075-44502022-07-0113763510.3390/insects13070635A Molecular Marker to Identify <i>Spodoptera frugiperda</i> (JE Smith) DNA in Predators’ Gut ContentDaniela Hipolito Maggio0Victória Zannuzzi Rossetti1Larissa Muniz Amaral Santos2Felipe Levorato Carmezini3Alberto Soares Corrêa4Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Av. Pádua Dias, 11, Piracicaba 13418-900, SP, BrazilLuiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Av. Pádua Dias, 11, Piracicaba 13418-900, SP, BrazilLuiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Av. Pádua Dias, 11, Piracicaba 13418-900, SP, BrazilLuiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Av. Pádua Dias, 11, Piracicaba 13418-900, SP, BrazilLuiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Av. Pádua Dias, 11, Piracicaba 13418-900, SP, Brazil<i>Spodoptera frugiperda</i> is a serious pest of maize and other crops worldwide. The integration of control tactics is recommended for <i>S. frugiperda</i> suppression because reports of insecticide and Btplant-resistance are frequent. Biological control agents would be an alternative to improve <i>S. frugiperda</i> control in agricultural areas. We constructed a species-specific molecular marker to detect <i>S. frugiperda</i> DNA in predators’ gut content and estimated the predation rates of ladybugs and earwigs on <i>S. frugiperda</i> in maize crops. Predators were sampled in Pirassununga, São Paulo state, Brazil, in 2020 and 2021. Using the species-specific molecular marker in laboratory conditions, we estimated the half-life time to detect <i>S. frugiperda</i> DNA in the gut contents of <i>Hippodamia convergens</i> as 6.16 h and <i>Doru luteipes</i> as 25.72 h. The weekly predation rate of <i>S. frugiperda</i> by predators in maize crop varied from 0 to 42.1% by ladybugs and from 0 to 9.2% by <i>D. luteipes</i>. Predation events on <i>S. frugiperda</i> by predators were more frequent during the maize reproductive stage. Our results confirmed that predators might contribute to <i>S. frugiperda</i> suppression in maize fields. However, further studies of prey–predator interactions and agricultural landscapes are essential for a better understanding of predator dynamics in crops.https://www.mdpi.com/2075-4450/13/7/635earwigsladybugshalf-life detectabilitybiological controlpredator–prey interaction |
| spellingShingle | Daniela Hipolito Maggio Victória Zannuzzi Rossetti Larissa Muniz Amaral Santos Felipe Levorato Carmezini Alberto Soares Corrêa A Molecular Marker to Identify <i>Spodoptera frugiperda</i> (JE Smith) DNA in Predators’ Gut Content Insects earwigs ladybugs half-life detectability biological control predator–prey interaction |
| title | A Molecular Marker to Identify <i>Spodoptera frugiperda</i> (JE Smith) DNA in Predators’ Gut Content |
| title_full | A Molecular Marker to Identify <i>Spodoptera frugiperda</i> (JE Smith) DNA in Predators’ Gut Content |
| title_fullStr | A Molecular Marker to Identify <i>Spodoptera frugiperda</i> (JE Smith) DNA in Predators’ Gut Content |
| title_full_unstemmed | A Molecular Marker to Identify <i>Spodoptera frugiperda</i> (JE Smith) DNA in Predators’ Gut Content |
| title_short | A Molecular Marker to Identify <i>Spodoptera frugiperda</i> (JE Smith) DNA in Predators’ Gut Content |
| title_sort | molecular marker to identify i spodoptera frugiperda i je smith dna in predators gut content |
| topic | earwigs ladybugs half-life detectability biological control predator–prey interaction |
| url | https://www.mdpi.com/2075-4450/13/7/635 |
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