<i>Lacticaseibacillus rhamnosus</i> Strain GG (LGG) Regulate Gut Microbial Metabolites, an In Vitro Study Using Three Mature Human Gut Microbial Cultures in a Simulator of Human Intestinal Microbial Ecosystem (SHIME)
In the present research, we investigated changes in the gut metabolome that occurred in response to the administration of the <i>Laticaseibacillus rhamnosus</i> strain GG (LGG). The probiotics were added to the ascending colon region of mature microbial communities established in a human...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/11/2105 |
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| author | LinShu Liu Adrienne B. Narrowe Jenni A. Firrman Karley K. Mahalak Jamshed T. Bobokalonov Johanna M. S. Lemons Kyle Bittinger Scott Daniel Ceylan Tanes Lisa Mattei Elliot S. Friedman Jason W. Soares Masuko Kobori Wei-Bin Zeng Peggy M. Tomasula |
| author_facet | LinShu Liu Adrienne B. Narrowe Jenni A. Firrman Karley K. Mahalak Jamshed T. Bobokalonov Johanna M. S. Lemons Kyle Bittinger Scott Daniel Ceylan Tanes Lisa Mattei Elliot S. Friedman Jason W. Soares Masuko Kobori Wei-Bin Zeng Peggy M. Tomasula |
| author_sort | LinShu Liu |
| collection | DOAJ |
| description | In the present research, we investigated changes in the gut metabolome that occurred in response to the administration of the <i>Laticaseibacillus rhamnosus</i> strain GG (LGG). The probiotics were added to the ascending colon region of mature microbial communities established in a human intestinal microbial ecosystem simulator. Shotgun metagenomic sequencing and metabolome analysis suggested that the changes in microbial community composition corresponded with changes to metabolic output, and we can infer linkages between some metabolites and microorganisms. The in vitro method permits a spatially-resolved view of metabolic transformations under human physiological conditions. By this method, we found that tryptophan and tyrosine were mainly produced in the ascending colon region, while their derivatives were detected in the transverse and descending regions, revealing sequential amino acid metabolic pathways along with the colonic tract. The addition of LGG appeared to promote the production of indole propionic acid, which is positively associated with human health. Furthermore, the microbial community responsible for the production of indole propionic acid may be broader than is currently known. |
| first_indexed | 2024-03-11T03:07:54Z |
| format | Article |
| id | doaj.art-b8984645ea314386b7656ca38c92b2cb |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T03:07:54Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-b8984645ea314386b7656ca38c92b2cb2023-11-18T07:50:20ZengMDPI AGFoods2304-81582023-05-011211210510.3390/foods12112105<i>Lacticaseibacillus rhamnosus</i> Strain GG (LGG) Regulate Gut Microbial Metabolites, an In Vitro Study Using Three Mature Human Gut Microbial Cultures in a Simulator of Human Intestinal Microbial Ecosystem (SHIME)LinShu Liu0Adrienne B. Narrowe1Jenni A. Firrman2Karley K. Mahalak3Jamshed T. Bobokalonov4Johanna M. S. Lemons5Kyle Bittinger6Scott Daniel7Ceylan Tanes8Lisa Mattei9Elliot S. Friedman10Jason W. Soares11Masuko Kobori12Wei-Bin Zeng13Peggy M. Tomasula14Dairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADivision of Gastroenterology, Hepatology, and Nutrition, The Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USADivision of Gastroenterology, Hepatology, and Nutrition, The Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USADivision of Gastroenterology, Hepatology, and Nutrition, The Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USADivision of Gastroenterology, Hepatology, and Nutrition, The Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USADivision of Gastroenterology & Hepatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USABioprocessing and Bioengineering Group, US Army Combat Capabilities Development Command Soldier Center (CCDC-SC), Natick, MA 01760, USAFood Research Institute, National Agriculture and Food Research Organization, Tsukuba 305-8642, Ibaraki, JapanDepartment of Mathematics, University of Louisville, Louisville, KY 40292, USADairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USAIn the present research, we investigated changes in the gut metabolome that occurred in response to the administration of the <i>Laticaseibacillus rhamnosus</i> strain GG (LGG). The probiotics were added to the ascending colon region of mature microbial communities established in a human intestinal microbial ecosystem simulator. Shotgun metagenomic sequencing and metabolome analysis suggested that the changes in microbial community composition corresponded with changes to metabolic output, and we can infer linkages between some metabolites and microorganisms. The in vitro method permits a spatially-resolved view of metabolic transformations under human physiological conditions. By this method, we found that tryptophan and tyrosine were mainly produced in the ascending colon region, while their derivatives were detected in the transverse and descending regions, revealing sequential amino acid metabolic pathways along with the colonic tract. The addition of LGG appeared to promote the production of indole propionic acid, which is positively associated with human health. Furthermore, the microbial community responsible for the production of indole propionic acid may be broader than is currently known.https://www.mdpi.com/2304-8158/12/11/2105<i>Laticaseibacillus rhamnosus</i> strain GGLGGSHIME<sup>®</sup>metabolomegut microbiomeindole propionic acid |
| spellingShingle | LinShu Liu Adrienne B. Narrowe Jenni A. Firrman Karley K. Mahalak Jamshed T. Bobokalonov Johanna M. S. Lemons Kyle Bittinger Scott Daniel Ceylan Tanes Lisa Mattei Elliot S. Friedman Jason W. Soares Masuko Kobori Wei-Bin Zeng Peggy M. Tomasula <i>Lacticaseibacillus rhamnosus</i> Strain GG (LGG) Regulate Gut Microbial Metabolites, an In Vitro Study Using Three Mature Human Gut Microbial Cultures in a Simulator of Human Intestinal Microbial Ecosystem (SHIME) Foods <i>Laticaseibacillus rhamnosus</i> strain GG LGG SHIME<sup>®</sup> metabolome gut microbiome indole propionic acid |
| title | <i>Lacticaseibacillus rhamnosus</i> Strain GG (LGG) Regulate Gut Microbial Metabolites, an In Vitro Study Using Three Mature Human Gut Microbial Cultures in a Simulator of Human Intestinal Microbial Ecosystem (SHIME) |
| title_full | <i>Lacticaseibacillus rhamnosus</i> Strain GG (LGG) Regulate Gut Microbial Metabolites, an In Vitro Study Using Three Mature Human Gut Microbial Cultures in a Simulator of Human Intestinal Microbial Ecosystem (SHIME) |
| title_fullStr | <i>Lacticaseibacillus rhamnosus</i> Strain GG (LGG) Regulate Gut Microbial Metabolites, an In Vitro Study Using Three Mature Human Gut Microbial Cultures in a Simulator of Human Intestinal Microbial Ecosystem (SHIME) |
| title_full_unstemmed | <i>Lacticaseibacillus rhamnosus</i> Strain GG (LGG) Regulate Gut Microbial Metabolites, an In Vitro Study Using Three Mature Human Gut Microbial Cultures in a Simulator of Human Intestinal Microbial Ecosystem (SHIME) |
| title_short | <i>Lacticaseibacillus rhamnosus</i> Strain GG (LGG) Regulate Gut Microbial Metabolites, an In Vitro Study Using Three Mature Human Gut Microbial Cultures in a Simulator of Human Intestinal Microbial Ecosystem (SHIME) |
| title_sort | i lacticaseibacillus rhamnosus i strain gg lgg regulate gut microbial metabolites an in vitro study using three mature human gut microbial cultures in a simulator of human intestinal microbial ecosystem shime |
| topic | <i>Laticaseibacillus rhamnosus</i> strain GG LGG SHIME<sup>®</sup> metabolome gut microbiome indole propionic acid |
| url | https://www.mdpi.com/2304-8158/12/11/2105 |
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