Cyclic AMP responsive element binding proteins are involved in 'emergency' granulopoiesis through the upregulation of CCAAT/enhancer binding protein β.
In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. In this study, a novel lentivirus-ba...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3559830?pdf=render |
| _version_ | 1811234400408436736 |
|---|---|
| author | Hideyo Hirai Naoka Kamio Gang Huang Akiko Matsusue Shinpei Ogino Nobuhiko Kimura Sakiko Satake Eishi Ashihara Jiro Imanishi Daniel G Tenen Taira Maekawa |
| author_facet | Hideyo Hirai Naoka Kamio Gang Huang Akiko Matsusue Shinpei Ogino Nobuhiko Kimura Sakiko Satake Eishi Ashihara Jiro Imanishi Daniel G Tenen Taira Maekawa |
| author_sort | Hideyo Hirai |
| collection | DOAJ |
| description | In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. In this study, a novel lentivirus-based reporter system was developed to elucidate the molecular switch required for C/EBPβ-dependency. The results demonstrated that two cyclic AMP responsive elements (CREs) in the proximal promoter region of C/EBPβ were involved in the positive regulation of C/EBPβ transcription during granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced differentiation of bone marrow cells. In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. Retroviral transduction of a dominant negative CREB mutant reduced C/EBPβ mRNA levels and significantly impaired the proliferation/differentiation of granulocyte precursors, while a constitutively active form of CREB facilitated C/EBPβ transcription. These data suggest that CREB proteins are involved in the regulation of granulopoiesis via C/EBPβ upregulation. |
| first_indexed | 2024-04-12T11:36:45Z |
| format | Article |
| id | doaj.art-b899ac3fe0934933b3a0ad882d586a49 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:36:45Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b899ac3fe0934933b3a0ad882d586a492022-12-22T03:34:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5486210.1371/journal.pone.0054862Cyclic AMP responsive element binding proteins are involved in 'emergency' granulopoiesis through the upregulation of CCAAT/enhancer binding protein β.Hideyo HiraiNaoka KamioGang HuangAkiko MatsusueShinpei OginoNobuhiko KimuraSakiko SatakeEishi AshiharaJiro ImanishiDaniel G TenenTaira MaekawaIn contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. In this study, a novel lentivirus-based reporter system was developed to elucidate the molecular switch required for C/EBPβ-dependency. The results demonstrated that two cyclic AMP responsive elements (CREs) in the proximal promoter region of C/EBPβ were involved in the positive regulation of C/EBPβ transcription during granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced differentiation of bone marrow cells. In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. Retroviral transduction of a dominant negative CREB mutant reduced C/EBPβ mRNA levels and significantly impaired the proliferation/differentiation of granulocyte precursors, while a constitutively active form of CREB facilitated C/EBPβ transcription. These data suggest that CREB proteins are involved in the regulation of granulopoiesis via C/EBPβ upregulation.http://europepmc.org/articles/PMC3559830?pdf=render |
| spellingShingle | Hideyo Hirai Naoka Kamio Gang Huang Akiko Matsusue Shinpei Ogino Nobuhiko Kimura Sakiko Satake Eishi Ashihara Jiro Imanishi Daniel G Tenen Taira Maekawa Cyclic AMP responsive element binding proteins are involved in 'emergency' granulopoiesis through the upregulation of CCAAT/enhancer binding protein β. PLoS ONE |
| title | Cyclic AMP responsive element binding proteins are involved in 'emergency' granulopoiesis through the upregulation of CCAAT/enhancer binding protein β. |
| title_full | Cyclic AMP responsive element binding proteins are involved in 'emergency' granulopoiesis through the upregulation of CCAAT/enhancer binding protein β. |
| title_fullStr | Cyclic AMP responsive element binding proteins are involved in 'emergency' granulopoiesis through the upregulation of CCAAT/enhancer binding protein β. |
| title_full_unstemmed | Cyclic AMP responsive element binding proteins are involved in 'emergency' granulopoiesis through the upregulation of CCAAT/enhancer binding protein β. |
| title_short | Cyclic AMP responsive element binding proteins are involved in 'emergency' granulopoiesis through the upregulation of CCAAT/enhancer binding protein β. |
| title_sort | cyclic amp responsive element binding proteins are involved in emergency granulopoiesis through the upregulation of ccaat enhancer binding protein β |
| url | http://europepmc.org/articles/PMC3559830?pdf=render |
| work_keys_str_mv | AT hideyohirai cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT naokakamio cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT ganghuang cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT akikomatsusue cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT shinpeiogino cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT nobuhikokimura cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT sakikosatake cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT eishiashihara cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT jiroimanishi cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT danielgtenen cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb AT tairamaekawa cyclicampresponsiveelementbindingproteinsareinvolvedinemergencygranulopoiesisthroughtheupregulationofccaatenhancerbindingproteinb |