Protective Effect of Aloe Polysaccharide on Oxidative Stress Injury of HepG2 Cells Induced by D-galactose
Objective: To investigate the protective effect of aloe polysaccharide on oxidative damage in HepG2 cells, and analyze its antioxidant capacity in vitro. Methods: HepG2 cells were used as the study target, to establish D-galactose-induced cell oxidation damage model and pre-protected with different...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022040037 |
| _version_ | 1828085329866784768 |
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| author | Yuezhu ZHAO Xin JIN Yunan ZHANG Jingshuang LI Yang YU |
| author_facet | Yuezhu ZHAO Xin JIN Yunan ZHANG Jingshuang LI Yang YU |
| author_sort | Yuezhu ZHAO |
| collection | DOAJ |
| description | Objective: To investigate the protective effect of aloe polysaccharide on oxidative damage in HepG2 cells, and analyze its antioxidant capacity in vitro. Methods: HepG2 cells were used as the study target, to establish D-galactose-induced cell oxidation damage model and pre-protected with different concentrations of aloe polysaccharide. Cell Counting Kit-8 was used to determine the survival rate of HepG2 cells, biochemical assays were performed to detect the cellular superoxide dismutase, catalase and glutathione peroxidase activities, enzyme-linked immunosorbent assay was used to determine the total antioxidant capacity and malondialdehyde content. Quantitative real-time PCR was used to detect the cellular Keap1, Nrf2, NQO1 and HO-1 gene expression levels. Results: Compared with the D-galactose model group, the pre-protection with 25, 50, 100 μg/mL of aloe polysaccharide increased the survival rate of HepG2 cells to different degrees. Aloe polysaccharide significantly enhanced the activity of antioxidant enzymes in cells and reduced the production of malondialdehyde in cells (P<0.05), and the effect was proportional to the concentration of aloe polysaccharide. The expression of Keap1 in cells was significantly reduced, and the expression of Nrf2, NQO1 and HO-1 in cells was significantly increased (P<0.05). Conclusion: Aloe polysaccharide can reduce oxidative stress damage in HepG2 cells, activate Nrf2 expression and inhibit its ubiquitination degradation, increase the transcription level of downstream NQO1 and HO-1, enhance the activity of cellular antioxidant enzymes and regulate the cellular redox system, to reduce the degree of oxidative damage and improve the effectiveness of cellular resistance to oxidative stress damage. |
| first_indexed | 2024-04-11T04:38:18Z |
| format | Article |
| id | doaj.art-b89a57dfe67547adb79f186df864a73a |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T04:38:18Z |
| publishDate | 2023-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-b89a57dfe67547adb79f186df864a73a2022-12-28T09:03:11ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-01-0144140541210.13386/j.issn1002-0306.20220400372022040037-1Protective Effect of Aloe Polysaccharide on Oxidative Stress Injury of HepG2 Cells Induced by D-galactoseYuezhu ZHAO0Xin JIN1Yunan ZHANG2Jingshuang LI3Yang YU4College of Food and Health, Jinzhou Medical University, Jinzhou 121001, ChinaCollege of Food and Health, Jinzhou Medical University, Jinzhou 121001, ChinaCollege of Food and Health, Jinzhou Medical University, Jinzhou 121001, ChinaCollege of Animal Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou 121001, ChinaCollege of Food and Health, Jinzhou Medical University, Jinzhou 121001, ChinaObjective: To investigate the protective effect of aloe polysaccharide on oxidative damage in HepG2 cells, and analyze its antioxidant capacity in vitro. Methods: HepG2 cells were used as the study target, to establish D-galactose-induced cell oxidation damage model and pre-protected with different concentrations of aloe polysaccharide. Cell Counting Kit-8 was used to determine the survival rate of HepG2 cells, biochemical assays were performed to detect the cellular superoxide dismutase, catalase and glutathione peroxidase activities, enzyme-linked immunosorbent assay was used to determine the total antioxidant capacity and malondialdehyde content. Quantitative real-time PCR was used to detect the cellular Keap1, Nrf2, NQO1 and HO-1 gene expression levels. Results: Compared with the D-galactose model group, the pre-protection with 25, 50, 100 μg/mL of aloe polysaccharide increased the survival rate of HepG2 cells to different degrees. Aloe polysaccharide significantly enhanced the activity of antioxidant enzymes in cells and reduced the production of malondialdehyde in cells (P<0.05), and the effect was proportional to the concentration of aloe polysaccharide. The expression of Keap1 in cells was significantly reduced, and the expression of Nrf2, NQO1 and HO-1 in cells was significantly increased (P<0.05). Conclusion: Aloe polysaccharide can reduce oxidative stress damage in HepG2 cells, activate Nrf2 expression and inhibit its ubiquitination degradation, increase the transcription level of downstream NQO1 and HO-1, enhance the activity of cellular antioxidant enzymes and regulate the cellular redox system, to reduce the degree of oxidative damage and improve the effectiveness of cellular resistance to oxidative stress damage.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022040037aloe polysaccharided-galactoseoxidative stresshepg2 cellsnrf2 |
| spellingShingle | Yuezhu ZHAO Xin JIN Yunan ZHANG Jingshuang LI Yang YU Protective Effect of Aloe Polysaccharide on Oxidative Stress Injury of HepG2 Cells Induced by D-galactose Shipin gongye ke-ji aloe polysaccharide d-galactose oxidative stress hepg2 cells nrf2 |
| title | Protective Effect of Aloe Polysaccharide on Oxidative Stress Injury of HepG2 Cells Induced by D-galactose |
| title_full | Protective Effect of Aloe Polysaccharide on Oxidative Stress Injury of HepG2 Cells Induced by D-galactose |
| title_fullStr | Protective Effect of Aloe Polysaccharide on Oxidative Stress Injury of HepG2 Cells Induced by D-galactose |
| title_full_unstemmed | Protective Effect of Aloe Polysaccharide on Oxidative Stress Injury of HepG2 Cells Induced by D-galactose |
| title_short | Protective Effect of Aloe Polysaccharide on Oxidative Stress Injury of HepG2 Cells Induced by D-galactose |
| title_sort | protective effect of aloe polysaccharide on oxidative stress injury of hepg2 cells induced by d galactose |
| topic | aloe polysaccharide d-galactose oxidative stress hepg2 cells nrf2 |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022040037 |
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