Poly(ethylene imine)-chitosan carbon dots: study of its physical–chemical properties and biological in vitro performance
Abstract Carbon dots (CDs) have been quickly extended for nanomedicine uses because of their multiple applications, such as bioimaging, sensors, and drug delivery. However, the interest in increasing their photoluminescence properties is not always accompanied by cytocompatibility. Thus, a knowledge...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2023-10-01
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| Series: | Discover Nano |
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| Online Access: | https://doi.org/10.1186/s11671-023-03907-4 |
| _version_ | 1797451781376049152 |
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| author | Nicolás Santos Santiago Valenzuela Camilo Segura Igor Osorio-Roman Macarena S. Arrázola Concepción Panadero-Medianero Paula A. Santana Manuel Ahumada |
| author_facet | Nicolás Santos Santiago Valenzuela Camilo Segura Igor Osorio-Roman Macarena S. Arrázola Concepción Panadero-Medianero Paula A. Santana Manuel Ahumada |
| author_sort | Nicolás Santos |
| collection | DOAJ |
| description | Abstract Carbon dots (CDs) have been quickly extended for nanomedicine uses because of their multiple applications, such as bioimaging, sensors, and drug delivery. However, the interest in increasing their photoluminescence properties is not always accompanied by cytocompatibility. Thus, a knowledge gap exists regarding their interactions with biological systems linked to the selected formulations and synthesis methods. In this work, we have developed carbon dots (CDs) based on poly (ethylene imine) (PEI) and chitosan (CS) by using microwave irradiation, hydrothermal synthesis, and a combination of both, and further characterized them by physicochemical and biological means. Our results indicate that synthesized CDs have sizes between 1 and 5 nm, a high presence of amine groups on the surface, and increased positive ζ potential values. Further, it is established that the choice and use of different synthesis procedures can contribute to a different answer to the CDs regarding their optical and biological properties. In this regard, PEI-only CDs showed the longest photoluminescent emission lifetime, non-hemolytic activity, and high toxicity against fibroblast. On the other hand, CS-only CDs have higher PL emission, non-cytotoxicity associated with fibroblast, and high hemolytic activity. Interestingly, their combination using the proposed methodologies allow a synergic effect in their CDs properties. Therefore, this work contributes to developing and characterizing CD formulations based on PEI and CS and better understanding the CD’s properties and biological interaction. |
| first_indexed | 2024-03-09T14:59:21Z |
| format | Article |
| id | doaj.art-b89aa27a2183452998839158bfd511e2 |
| institution | Directory Open Access Journal |
| issn | 2731-9229 |
| language | English |
| last_indexed | 2024-03-09T14:59:21Z |
| publishDate | 2023-10-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Nano |
| spelling | doaj.art-b89aa27a2183452998839158bfd511e22023-11-26T14:01:15ZengSpringerDiscover Nano2731-92292023-10-0118111410.1186/s11671-023-03907-4Poly(ethylene imine)-chitosan carbon dots: study of its physical–chemical properties and biological in vitro performanceNicolás Santos0Santiago Valenzuela1Camilo Segura2Igor Osorio-Roman3Macarena S. Arrázola4Concepción Panadero-Medianero5Paula A. Santana6Manuel Ahumada7Escuela de Biotecnología, Facultad de Ciencias, Ingeniería y Tecnología, Universidad MayorInstituto de Ciencias Aplicadas, Universidad Autónoma de ChileInstituto de Ciencias Químicas, Facultad de Ciencias, Universidad Austral de ChileInstituto de Ciencias Químicas, Facultad de Ciencias, Universidad Austral de ChileCentro de Biología Integrativa, Facultad de Ciencias, Ingeniería y Tecnología, Universidad MayorCentro de Biología Integrativa, Facultad de Ciencias, Ingeniería y Tecnología, Universidad MayorInstituto de Ciencias Aplicadas, Universidad Autónoma de ChileEscuela de Biotecnología, Facultad de Ciencias, Ingeniería y Tecnología, Universidad MayorAbstract Carbon dots (CDs) have been quickly extended for nanomedicine uses because of their multiple applications, such as bioimaging, sensors, and drug delivery. However, the interest in increasing their photoluminescence properties is not always accompanied by cytocompatibility. Thus, a knowledge gap exists regarding their interactions with biological systems linked to the selected formulations and synthesis methods. In this work, we have developed carbon dots (CDs) based on poly (ethylene imine) (PEI) and chitosan (CS) by using microwave irradiation, hydrothermal synthesis, and a combination of both, and further characterized them by physicochemical and biological means. Our results indicate that synthesized CDs have sizes between 1 and 5 nm, a high presence of amine groups on the surface, and increased positive ζ potential values. Further, it is established that the choice and use of different synthesis procedures can contribute to a different answer to the CDs regarding their optical and biological properties. In this regard, PEI-only CDs showed the longest photoluminescent emission lifetime, non-hemolytic activity, and high toxicity against fibroblast. On the other hand, CS-only CDs have higher PL emission, non-cytotoxicity associated with fibroblast, and high hemolytic activity. Interestingly, their combination using the proposed methodologies allow a synergic effect in their CDs properties. Therefore, this work contributes to developing and characterizing CD formulations based on PEI and CS and better understanding the CD’s properties and biological interaction.https://doi.org/10.1186/s11671-023-03907-4Carbon dotsPoly (ethylene imine)ChitosanCytotoxicityAntibacterial activity |
| spellingShingle | Nicolás Santos Santiago Valenzuela Camilo Segura Igor Osorio-Roman Macarena S. Arrázola Concepción Panadero-Medianero Paula A. Santana Manuel Ahumada Poly(ethylene imine)-chitosan carbon dots: study of its physical–chemical properties and biological in vitro performance Discover Nano Carbon dots Poly (ethylene imine) Chitosan Cytotoxicity Antibacterial activity |
| title | Poly(ethylene imine)-chitosan carbon dots: study of its physical–chemical properties and biological in vitro performance |
| title_full | Poly(ethylene imine)-chitosan carbon dots: study of its physical–chemical properties and biological in vitro performance |
| title_fullStr | Poly(ethylene imine)-chitosan carbon dots: study of its physical–chemical properties and biological in vitro performance |
| title_full_unstemmed | Poly(ethylene imine)-chitosan carbon dots: study of its physical–chemical properties and biological in vitro performance |
| title_short | Poly(ethylene imine)-chitosan carbon dots: study of its physical–chemical properties and biological in vitro performance |
| title_sort | poly ethylene imine chitosan carbon dots study of its physical chemical properties and biological in vitro performance |
| topic | Carbon dots Poly (ethylene imine) Chitosan Cytotoxicity Antibacterial activity |
| url | https://doi.org/10.1186/s11671-023-03907-4 |
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