Functional expression of kinin B1 and B2 receptors in mouse abdominal aorta
Previous studies have shown that the vascular reactivity of the mouse aorta differs substantially from that of the rat aorta in response to several agonists such as angiotensin II, endothelin-1 and isoproterenol. However, no information is available about the agonists bradykinin (BK) and DesArg9BK (...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2007-05-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500007 |
| _version_ | 1818935558721765376 |
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| author | S.A. Felipe E.S. Rodrigues R.P. Martin A.C.M. Paiva J.B. Pesquero S.I. Shimuta |
| author_facet | S.A. Felipe E.S. Rodrigues R.P. Martin A.C.M. Paiva J.B. Pesquero S.I. Shimuta |
| author_sort | S.A. Felipe |
| collection | DOAJ |
| description | Previous studies have shown that the vascular reactivity of the mouse aorta differs substantially from that of the rat aorta in response to several agonists such as angiotensin II, endothelin-1 and isoproterenol. However, no information is available about the agonists bradykinin (BK) and DesArg9BK (DBK). Our aim was to determine the potential expression of kinin B1 and B2 receptors in the abdominal mouse aorta isolated from C57BL/6 mice. Contraction and relaxation responses to BK and DBK were investigated using isometric recordings. The kinins were unable to induce relaxation but concentration-contraction response curves were obtained by applying increasing concentrations of the agonists BK and DBK. These effects were blocked by the antagonists Icatibant and R-715, respectively. The potency (pD2) calculated from the curves was 7.0 ± 0.1 for BK and 7.3 ± 0.2 for DBK. The efficacy was 51 ± 2% for BK and 30 ± 1% for DBK when compared to 1 µM norepinephrine. The concentration-dependent responses of BK and DBK were markedly inhibited by the arachidonic acid inhibitor indomethacin (1 µM), suggesting a mediation by the cyclooxygenase pathway. These contractile responses were not potentiated in the presence of the NOS inhibitor L-NAME (1 mM) or endothelium-denuded aorta, indicating that the NO pathway is not involved. We conclude that the mouse aorta constitutively contains B1 and B2 subtypes of kinin receptors and that stimulation with BK and DBK induces contractile effect mediated by endothelium-independent vasoconstrictor prostanoids. |
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| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-20T05:22:05Z |
| publishDate | 2007-05-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-b89ebce305fe4b70a59986b7303584d52022-12-21T19:51:59ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2007-05-0140564965510.1590/S0100-879X2007000500007Functional expression of kinin B1 and B2 receptors in mouse abdominal aortaS.A. FelipeE.S. RodriguesR.P. MartinA.C.M. PaivaJ.B. PesqueroS.I. ShimutaPrevious studies have shown that the vascular reactivity of the mouse aorta differs substantially from that of the rat aorta in response to several agonists such as angiotensin II, endothelin-1 and isoproterenol. However, no information is available about the agonists bradykinin (BK) and DesArg9BK (DBK). Our aim was to determine the potential expression of kinin B1 and B2 receptors in the abdominal mouse aorta isolated from C57BL/6 mice. Contraction and relaxation responses to BK and DBK were investigated using isometric recordings. The kinins were unable to induce relaxation but concentration-contraction response curves were obtained by applying increasing concentrations of the agonists BK and DBK. These effects were blocked by the antagonists Icatibant and R-715, respectively. The potency (pD2) calculated from the curves was 7.0 ± 0.1 for BK and 7.3 ± 0.2 for DBK. The efficacy was 51 ± 2% for BK and 30 ± 1% for DBK when compared to 1 µM norepinephrine. The concentration-dependent responses of BK and DBK were markedly inhibited by the arachidonic acid inhibitor indomethacin (1 µM), suggesting a mediation by the cyclooxygenase pathway. These contractile responses were not potentiated in the presence of the NOS inhibitor L-NAME (1 mM) or endothelium-denuded aorta, indicating that the NO pathway is not involved. We conclude that the mouse aorta constitutively contains B1 and B2 subtypes of kinin receptors and that stimulation with BK and DBK induces contractile effect mediated by endothelium-independent vasoconstrictor prostanoids.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500007Kinin B1 and B2 receptorsBradykininDesArg9bradykininIndomethacinL-NAME |
| spellingShingle | S.A. Felipe E.S. Rodrigues R.P. Martin A.C.M. Paiva J.B. Pesquero S.I. Shimuta Functional expression of kinin B1 and B2 receptors in mouse abdominal aorta Brazilian Journal of Medical and Biological Research Kinin B1 and B2 receptors Bradykinin DesArg9bradykinin Indomethacin L-NAME |
| title | Functional expression of kinin B1 and B2 receptors in mouse abdominal aorta |
| title_full | Functional expression of kinin B1 and B2 receptors in mouse abdominal aorta |
| title_fullStr | Functional expression of kinin B1 and B2 receptors in mouse abdominal aorta |
| title_full_unstemmed | Functional expression of kinin B1 and B2 receptors in mouse abdominal aorta |
| title_short | Functional expression of kinin B1 and B2 receptors in mouse abdominal aorta |
| title_sort | functional expression of kinin b1 and b2 receptors in mouse abdominal aorta |
| topic | Kinin B1 and B2 receptors Bradykinin DesArg9bradykinin Indomethacin L-NAME |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500007 |
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