Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis
ABSTRACT: Leishmaniasis represents a complex of chronic diseases with a broad geographic distribution and a high significance in public health worldwide. The varied clinical signs in conjunction with the low sensitivity and specificity of canine visceral leishmaniasis (CVL) detection methods make d...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2019-05-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500901&lng=en&tlng=en |
| _version_ | 1818178458307526656 |
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| author | Mário César Rennó Amanda Otoni Vasconcellos Rogerio Rodrigues Santos Rodrigo Otávio Silveira Silva Rubens Antônio Carneiro Paulo Ricardo de Oliveira Paes |
| author_facet | Mário César Rennó Amanda Otoni Vasconcellos Rogerio Rodrigues Santos Rodrigo Otávio Silveira Silva Rubens Antônio Carneiro Paulo Ricardo de Oliveira Paes |
| author_sort | Mário César Rennó |
| collection | DOAJ |
| description | ABSTRACT: Leishmaniasis represents a complex of chronic diseases with a broad geographic distribution and a high significance in public health worldwide. The varied clinical signs in conjunction with the low sensitivity and specificity of canine visceral leishmaniasis (CVL) detection methods make diagnosis of the disease complex. Among the several available laboratory tests, studies have suggested that the detection of parasites in synovial fluid (SF) is a good auxiliary tool in the diagnosis of CVL. However, no study has evaluated the influence of the clinical stage of CVL in the detection of Leishmania sp. in SF. This study aimed to evaluate the detection of Leishmania sp. amastigotes in the SF of dogs at different stages of the disease. The negative control group (G1) comprised 12 dogs that tested negative for CVL. Thirty-six other dogs, tested serologically positive for CVL, were divided into two groups: Group 2 (G2), which included animals at stage II of the disease (moderate; n=18), and Group 3 (G3) included animals at stage III of the disease (severe; n=18). The analysis of SF revealed the presence of parasites in six (33.3%) dogs from G2 and in 16 (88.9%) dogs from G3 (p=0.0437). The present research suggested that SF analysis is of high value as a supplementary tool in the diagnosis of CVL. As a new finding, the present study also indicated that this test has a higher sensitivity in animals presenting with more severe stage of the disease. |
| first_indexed | 2024-12-11T20:48:18Z |
| format | Article |
| id | doaj.art-b8a2a099ce7a40ab97b50a6ea8691c55 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-12-11T20:48:18Z |
| publishDate | 2019-05-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-b8a2a099ce7a40ab97b50a6ea8691c552022-12-22T00:51:19ZengUniversidade Federal de Santa MariaCiência Rural1678-45962019-05-0149510.1590/0103-8478cr20190023S0103-84782019000500901Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasisMário César RennóAmanda Otoni VasconcellosRogerio Rodrigues SantosRodrigo Otávio Silveira SilvaRubens Antônio CarneiroPaulo Ricardo de Oliveira PaesABSTRACT: Leishmaniasis represents a complex of chronic diseases with a broad geographic distribution and a high significance in public health worldwide. The varied clinical signs in conjunction with the low sensitivity and specificity of canine visceral leishmaniasis (CVL) detection methods make diagnosis of the disease complex. Among the several available laboratory tests, studies have suggested that the detection of parasites in synovial fluid (SF) is a good auxiliary tool in the diagnosis of CVL. However, no study has evaluated the influence of the clinical stage of CVL in the detection of Leishmania sp. in SF. This study aimed to evaluate the detection of Leishmania sp. amastigotes in the SF of dogs at different stages of the disease. The negative control group (G1) comprised 12 dogs that tested negative for CVL. Thirty-six other dogs, tested serologically positive for CVL, were divided into two groups: Group 2 (G2), which included animals at stage II of the disease (moderate; n=18), and Group 3 (G3) included animals at stage III of the disease (severe; n=18). The analysis of SF revealed the presence of parasites in six (33.3%) dogs from G2 and in 16 (88.9%) dogs from G3 (p=0.0437). The present research suggested that SF analysis is of high value as a supplementary tool in the diagnosis of CVL. As a new finding, the present study also indicated that this test has a higher sensitivity in animals presenting with more severe stage of the disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500901&lng=en&tlng=enLeishmania infantumzoonosis |
| spellingShingle | Mário César Rennó Amanda Otoni Vasconcellos Rogerio Rodrigues Santos Rodrigo Otávio Silveira Silva Rubens Antônio Carneiro Paulo Ricardo de Oliveira Paes Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis Ciência Rural Leishmania infantum zoonosis |
| title | Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis |
| title_full | Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis |
| title_fullStr | Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis |
| title_full_unstemmed | Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis |
| title_short | Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis |
| title_sort | synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis |
| topic | Leishmania infantum zoonosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500901&lng=en&tlng=en |
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