Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration
Abstract A variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-96271-1 |
| _version_ | 1819195130384482304 |
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| author | Sajad Bahrami Nafiseh Baheiraei Mostafa Shahrezaee |
| author_facet | Sajad Bahrami Nafiseh Baheiraei Mostafa Shahrezaee |
| author_sort | Sajad Bahrami |
| collection | DOAJ |
| description | Abstract A variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties for implantation in the injured area. In this study, we synthesized collagen (Col) and reduced graphene oxide coated collagen (Col-rGO) scaffolds, and we evaluated their in vitro and in vivo effects on bone tissue repair. Col and Col-rGO scaffolds were synthesized by chemical crosslinking and freeze-drying methods. The surface topography, and the mechanical and chemical properties of scaffolds were characterized, showing three-dimensional (3D) porous scaffolds and successful coating of rGO on Col. The rGO coating enhanced the mechanical strength of Col-rGO scaffolds to a greater extent than Col scaffolds by 2.8 times. Furthermore, Col-rGO scaffolds confirmed that graphene addition induced no cytotoxic effects and enhanced the viability and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) with 3D adherence and expansion. Finally, scaffold implantation into rabbit cranial bone defects for 12 weeks showed increased bone formation, confirmed by Hematoxylin–Eosin (H&E) and alizarin red staining. Overall, the study showed that rGO coating improves Col scaffold properties and could be a promising implant for bone injuries. |
| first_indexed | 2024-12-23T02:07:52Z |
| format | Article |
| id | doaj.art-b8a2e00066a247eca8dc34795b16320e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T02:07:52Z |
| publishDate | 2021-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b8a2e00066a247eca8dc34795b16320e2022-12-21T18:03:51ZengNature PortfolioScientific Reports2045-23222021-08-0111111010.1038/s41598-021-96271-1Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regenerationSajad Bahrami0Nafiseh Baheiraei1Mostafa Shahrezaee2Department of Orthopedic Surgery, AJA University of Medical SciencesTissue Engineering and Applied Cell Sciences Division, Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares UniversityDepartment of Orthopedic Surgery, AJA University of Medical SciencesAbstract A variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties for implantation in the injured area. In this study, we synthesized collagen (Col) and reduced graphene oxide coated collagen (Col-rGO) scaffolds, and we evaluated their in vitro and in vivo effects on bone tissue repair. Col and Col-rGO scaffolds were synthesized by chemical crosslinking and freeze-drying methods. The surface topography, and the mechanical and chemical properties of scaffolds were characterized, showing three-dimensional (3D) porous scaffolds and successful coating of rGO on Col. The rGO coating enhanced the mechanical strength of Col-rGO scaffolds to a greater extent than Col scaffolds by 2.8 times. Furthermore, Col-rGO scaffolds confirmed that graphene addition induced no cytotoxic effects and enhanced the viability and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) with 3D adherence and expansion. Finally, scaffold implantation into rabbit cranial bone defects for 12 weeks showed increased bone formation, confirmed by Hematoxylin–Eosin (H&E) and alizarin red staining. Overall, the study showed that rGO coating improves Col scaffold properties and could be a promising implant for bone injuries.https://doi.org/10.1038/s41598-021-96271-1 |
| spellingShingle | Sajad Bahrami Nafiseh Baheiraei Mostafa Shahrezaee Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration Scientific Reports |
| title | Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration |
| title_full | Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration |
| title_fullStr | Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration |
| title_full_unstemmed | Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration |
| title_short | Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration |
| title_sort | biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration |
| url | https://doi.org/10.1038/s41598-021-96271-1 |
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