d-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat‐stability and noteworthy potential of d-fructose epimerization
Abstract Background d-Allulose is an ultra-low calorie sugar of multifarious health benefits, including anti-diabetic and anti-obesity potential. d-Allulose 3-epimerase family enzymes catalyze biosynthesis of d-allulose via epimerization of d-fructose. Results A novel d-allulose 3-epimerase (DaeB) w...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-03-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-021-01550-1 |
| _version_ | 1818677567533613056 |
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| author | Satya Narayan Patel Girija Kaushal Sudhir P. Singh |
| author_facet | Satya Narayan Patel Girija Kaushal Sudhir P. Singh |
| author_sort | Satya Narayan Patel |
| collection | DOAJ |
| description | Abstract Background d-Allulose is an ultra-low calorie sugar of multifarious health benefits, including anti-diabetic and anti-obesity potential. d-Allulose 3-epimerase family enzymes catalyze biosynthesis of d-allulose via epimerization of d-fructose. Results A novel d-allulose 3-epimerase (DaeB) was cloned from a plant probiotic strain, Bacillus sp. KCTC 13219, and expressed in Bacillus subtilis cells. The purified protein exhibited substantial epimerization activity in a broad pH spectrum, 6.0–11.0. DaeB was able to catalyze d-fructose to d-allulose bioconversion at the temperature range of 35 °C to 70 °C, exhibiting at least 50 % activity. It displaced excessive heat stability, with the half-life of 25 days at 50 °C, and high turnover number (k cat 367 s− 1). The coupling of DaeB treatment and yeast fermentation of 700 g L− 1 d-fructose solution yielded approximately 200 g L− 1 d-allulose, and 214 g L− 1 ethanol. Conclusions The novel d-allulose 3-epimerase of Bacillus sp. origin discerned a high magnitude of heat stability along with exorbitant epimerization ability. This biocatalyst has enormous potential for the large-scale production of d-allulose. |
| first_indexed | 2024-12-17T09:01:25Z |
| format | Article |
| id | doaj.art-b8a566806a6d439fb9f23faef088e04c |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-17T09:01:25Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-b8a566806a6d439fb9f23faef088e04c2022-12-21T21:55:43ZengBMCMicrobial Cell Factories1475-28592021-03-0120111610.1186/s12934-021-01550-1d-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat‐stability and noteworthy potential of d-fructose epimerizationSatya Narayan Patel0Girija Kaushal1Sudhir P. Singh2Center of Innovative and Applied BioprocessingCenter of Innovative and Applied BioprocessingCenter of Innovative and Applied BioprocessingAbstract Background d-Allulose is an ultra-low calorie sugar of multifarious health benefits, including anti-diabetic and anti-obesity potential. d-Allulose 3-epimerase family enzymes catalyze biosynthesis of d-allulose via epimerization of d-fructose. Results A novel d-allulose 3-epimerase (DaeB) was cloned from a plant probiotic strain, Bacillus sp. KCTC 13219, and expressed in Bacillus subtilis cells. The purified protein exhibited substantial epimerization activity in a broad pH spectrum, 6.0–11.0. DaeB was able to catalyze d-fructose to d-allulose bioconversion at the temperature range of 35 °C to 70 °C, exhibiting at least 50 % activity. It displaced excessive heat stability, with the half-life of 25 days at 50 °C, and high turnover number (k cat 367 s− 1). The coupling of DaeB treatment and yeast fermentation of 700 g L− 1 d-fructose solution yielded approximately 200 g L− 1 d-allulose, and 214 g L− 1 ethanol. Conclusions The novel d-allulose 3-epimerase of Bacillus sp. origin discerned a high magnitude of heat stability along with exorbitant epimerization ability. This biocatalyst has enormous potential for the large-scale production of d-allulose.https://doi.org/10.1186/s12934-021-01550-1d-Allulosed-Allulose 3-epimeraseBacillus sp.ThermostabilityTurnover number |
| spellingShingle | Satya Narayan Patel Girija Kaushal Sudhir P. Singh d-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat‐stability and noteworthy potential of d-fructose epimerization Microbial Cell Factories d-Allulose d-Allulose 3-epimerase Bacillus sp. Thermostability Turnover number |
| title | d-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat‐stability and noteworthy potential of d-fructose epimerization |
| title_full | d-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat‐stability and noteworthy potential of d-fructose epimerization |
| title_fullStr | d-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat‐stability and noteworthy potential of d-fructose epimerization |
| title_full_unstemmed | d-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat‐stability and noteworthy potential of d-fructose epimerization |
| title_short | d-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat‐stability and noteworthy potential of d-fructose epimerization |
| title_sort | d allulose 3 epimerase of bacillus sp origin manifests profuse heat stability and noteworthy potential of d fructose epimerization |
| topic | d-Allulose d-Allulose 3-epimerase Bacillus sp. Thermostability Turnover number |
| url | https://doi.org/10.1186/s12934-021-01550-1 |
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