Astrovirus-Associated Polioencephalomyelitis in an Alpaca
An 8-year-old alpaca was admitted to the emergency service of the Clinic for Ruminants in Bern due to a reduced general condition and progressive neurological signs. Despite supportive treatment, its condition deteriorated and the animal had to be euthanized. Histopathological analysis revealed a se...
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MDPI AG
2020-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/1/50 |
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| author | Leonore Küchler Isabelle Rüfli Michel C. Koch Melanie M. Hierweger Ronja V. Kauer Céline L. Boujon Monika Hilbe Anna Oevermann Patrik Zanolari Torsten Seuberlich Corinne Gurtner |
| author_facet | Leonore Küchler Isabelle Rüfli Michel C. Koch Melanie M. Hierweger Ronja V. Kauer Céline L. Boujon Monika Hilbe Anna Oevermann Patrik Zanolari Torsten Seuberlich Corinne Gurtner |
| author_sort | Leonore Küchler |
| collection | DOAJ |
| description | An 8-year-old alpaca was admitted to the emergency service of the Clinic for Ruminants in Bern due to a reduced general condition and progressive neurological signs. Despite supportive treatment, its condition deteriorated and the animal had to be euthanized. Histopathological analysis revealed a severe non-suppurative polioencephalomyelitis with neuronal necrosis, most likely of viral origin. We detected abundant neuronal labelling with antibodies directed against two different epitopes of Bovine Astrovirus CH13/NeuroS1 (BoAstV-CH13/NeuroS1), which is a common viral agent associated with non-suppurative encephalitis in Swiss cattle. These findings were further verified by detection of viral RNA by use of in-situ hybridization and real-time RT-PCR. Next generation sequencing revealed that the detected virus genome had a pairwise identity of 98.9% to the genome of BoAstV-CH13/NeuroS1. To our knowledge, this is the first report of an astrovirus-associated polioencephalomyelitis in an alpaca. These results point to the possibility of an interspecies transmission of BoAstV-CH13/NeuroS1. |
| first_indexed | 2024-03-10T13:39:59Z |
| format | Article |
| id | doaj.art-b8a5d5425c434a71affdde950dfeefd7 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T13:39:59Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-b8a5d5425c434a71affdde950dfeefd72023-11-21T03:10:15ZengMDPI AGViruses1999-49152020-12-011315010.3390/v13010050Astrovirus-Associated Polioencephalomyelitis in an AlpacaLeonore Küchler0Isabelle Rüfli1Michel C. Koch2Melanie M. Hierweger3Ronja V. Kauer4Céline L. Boujon5Monika Hilbe6Anna Oevermann7Patrik Zanolari8Torsten Seuberlich9Corinne Gurtner10Institute of Veterinary Pathology, Vetsuisse-Faculty, Universitiy of Bern, 3012 Bern, SwitzerlandClinic for Ruminants, Vetsuisse-Faculty, University of Bern, 3012 Bern, SwitzerlandDivision of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, SwitzerlandDivision of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, SwitzerlandDivision of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, SwitzerlandDivision of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, SwitzerlandInstitute for Veterinary Pathology, Vetsuisse-Faculty, University of Zurich, 8006 Zurich, SwitzerlandDivision of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, SwitzerlandClinic for Ruminants, Vetsuisse-Faculty, University of Bern, 3012 Bern, SwitzerlandDivision of Experimental Clinical Research, Vetsuisse-Faculty, University of Bern, 3012 Bern, SwitzerlandInstitute of Veterinary Pathology, Vetsuisse-Faculty, Universitiy of Bern, 3012 Bern, SwitzerlandAn 8-year-old alpaca was admitted to the emergency service of the Clinic for Ruminants in Bern due to a reduced general condition and progressive neurological signs. Despite supportive treatment, its condition deteriorated and the animal had to be euthanized. Histopathological analysis revealed a severe non-suppurative polioencephalomyelitis with neuronal necrosis, most likely of viral origin. We detected abundant neuronal labelling with antibodies directed against two different epitopes of Bovine Astrovirus CH13/NeuroS1 (BoAstV-CH13/NeuroS1), which is a common viral agent associated with non-suppurative encephalitis in Swiss cattle. These findings were further verified by detection of viral RNA by use of in-situ hybridization and real-time RT-PCR. Next generation sequencing revealed that the detected virus genome had a pairwise identity of 98.9% to the genome of BoAstV-CH13/NeuroS1. To our knowledge, this is the first report of an astrovirus-associated polioencephalomyelitis in an alpaca. These results point to the possibility of an interspecies transmission of BoAstV-CH13/NeuroS1.https://www.mdpi.com/1999-4915/13/1/50astroviruspolioencephalomyelitisalpacaimmunohistochemistryreal-time RT-PCRnext generation sequencing |
| spellingShingle | Leonore Küchler Isabelle Rüfli Michel C. Koch Melanie M. Hierweger Ronja V. Kauer Céline L. Boujon Monika Hilbe Anna Oevermann Patrik Zanolari Torsten Seuberlich Corinne Gurtner Astrovirus-Associated Polioencephalomyelitis in an Alpaca Viruses astrovirus polioencephalomyelitis alpaca immunohistochemistry real-time RT-PCR next generation sequencing |
| title | Astrovirus-Associated Polioencephalomyelitis in an Alpaca |
| title_full | Astrovirus-Associated Polioencephalomyelitis in an Alpaca |
| title_fullStr | Astrovirus-Associated Polioencephalomyelitis in an Alpaca |
| title_full_unstemmed | Astrovirus-Associated Polioencephalomyelitis in an Alpaca |
| title_short | Astrovirus-Associated Polioencephalomyelitis in an Alpaca |
| title_sort | astrovirus associated polioencephalomyelitis in an alpaca |
| topic | astrovirus polioencephalomyelitis alpaca immunohistochemistry real-time RT-PCR next generation sequencing |
| url | https://www.mdpi.com/1999-4915/13/1/50 |
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