Modern Approaches to In Vitro Clonal Banana Production: Next-Generation Tissue Culture Systems
In this study, the solid culture method, and Plantform™ and SETIS™ temporary immersion bioreactor systems were used comparatively to propagate, root, and acclimatize ‘Grande Naine’ and ‘Azman’ banana varieties for rapid, cheap, and mass production in in vitro conditions. Micropropagation rate, plant...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/10/1154 |
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| author | Mansur Hakan Erol Dicle Dönmez Belgin Biçen Özhan Şimşek Yıldız Aka Kaçar |
| author_facet | Mansur Hakan Erol Dicle Dönmez Belgin Biçen Özhan Şimşek Yıldız Aka Kaçar |
| author_sort | Mansur Hakan Erol |
| collection | DOAJ |
| description | In this study, the solid culture method, and Plantform™ and SETIS™ temporary immersion bioreactor systems were used comparatively to propagate, root, and acclimatize ‘Grande Naine’ and ‘Azman’ banana varieties for rapid, cheap, and mass production in in vitro conditions. Micropropagation rate, plant height, number of leaves, and fresh and dry weight parameters were investigated in the micropropagation stage across eight subcultures. Rooting rate, plant height, number of leaves, number of roots/plant, root length, fresh and dry weight parameters were investigated in the rooting stage. Photosynthetic pigment analyses and stoma examinations were performed throughout all stages. In the micropropagation stage, a 20% increase in the Plantform™ system, a 12% increase in the SETIS™ system in ‘Grande Naine’, an 82% increase in the Plantform™ system, and a 98% increase in SETIS™ system in ‘Azman’ were determined compared to the solid culture. At the rooting stage, higher data were obtained from bioreactor systems than solid culture. Plants from bioreactor systems acclimatized faster and developed healthier in the greenhouse stage. It was determined that stomata were more active, and pigment accumulation was higher in bioreactor systems. Genetic variations across subcultures are among the most critical issues in banana clonal propagation. Leaf samples were taken from each system, and plant variation was investigated using SSR (Simple Sequence Repeat) markers. No variation was observed from the initial stage to the greenhouse stage. As a result, it has been determined that bioreactor systems are an essential alternative for the mass production of bananas. |
| first_indexed | 2024-03-10T21:13:02Z |
| format | Article |
| id | doaj.art-b8a6505b65ec4e68a003cdda0fd8e606 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T21:13:02Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-b8a6505b65ec4e68a003cdda0fd8e6062023-11-19T16:40:08ZengMDPI AGHorticulturae2311-75242023-10-01910115410.3390/horticulturae9101154Modern Approaches to In Vitro Clonal Banana Production: Next-Generation Tissue Culture SystemsMansur Hakan Erol0Dicle Dönmez1Belgin Biçen2Özhan Şimşek3Yıldız Aka Kaçar4Biotechnology Research and Application Center, Çukurova University, Adana 01330, TürkiyeBiotechnology Research and Application Center, Çukurova University, Adana 01330, TürkiyeYK Plant Tissue Culture Laboratory, Çukurova Technokent, Adana 01330, TürkiyeHorticulture Department, Agriculture Faculty, Erciyes University, Kayseri 39030, TürkiyeHorticulture Department, Agriculture Faculty, Çukurova University, Adana 01330, TürkiyeIn this study, the solid culture method, and Plantform™ and SETIS™ temporary immersion bioreactor systems were used comparatively to propagate, root, and acclimatize ‘Grande Naine’ and ‘Azman’ banana varieties for rapid, cheap, and mass production in in vitro conditions. Micropropagation rate, plant height, number of leaves, and fresh and dry weight parameters were investigated in the micropropagation stage across eight subcultures. Rooting rate, plant height, number of leaves, number of roots/plant, root length, fresh and dry weight parameters were investigated in the rooting stage. Photosynthetic pigment analyses and stoma examinations were performed throughout all stages. In the micropropagation stage, a 20% increase in the Plantform™ system, a 12% increase in the SETIS™ system in ‘Grande Naine’, an 82% increase in the Plantform™ system, and a 98% increase in SETIS™ system in ‘Azman’ were determined compared to the solid culture. At the rooting stage, higher data were obtained from bioreactor systems than solid culture. Plants from bioreactor systems acclimatized faster and developed healthier in the greenhouse stage. It was determined that stomata were more active, and pigment accumulation was higher in bioreactor systems. Genetic variations across subcultures are among the most critical issues in banana clonal propagation. Leaf samples were taken from each system, and plant variation was investigated using SSR (Simple Sequence Repeat) markers. No variation was observed from the initial stage to the greenhouse stage. As a result, it has been determined that bioreactor systems are an essential alternative for the mass production of bananas.https://www.mdpi.com/2311-7524/9/10/1154micropropagationphotosynthetic pigmentplantformrootingsetistemporary immersion system |
| spellingShingle | Mansur Hakan Erol Dicle Dönmez Belgin Biçen Özhan Şimşek Yıldız Aka Kaçar Modern Approaches to In Vitro Clonal Banana Production: Next-Generation Tissue Culture Systems Horticulturae micropropagation photosynthetic pigment plantform rooting setis temporary immersion system |
| title | Modern Approaches to In Vitro Clonal Banana Production: Next-Generation Tissue Culture Systems |
| title_full | Modern Approaches to In Vitro Clonal Banana Production: Next-Generation Tissue Culture Systems |
| title_fullStr | Modern Approaches to In Vitro Clonal Banana Production: Next-Generation Tissue Culture Systems |
| title_full_unstemmed | Modern Approaches to In Vitro Clonal Banana Production: Next-Generation Tissue Culture Systems |
| title_short | Modern Approaches to In Vitro Clonal Banana Production: Next-Generation Tissue Culture Systems |
| title_sort | modern approaches to in vitro clonal banana production next generation tissue culture systems |
| topic | micropropagation photosynthetic pigment plantform rooting setis temporary immersion system |
| url | https://www.mdpi.com/2311-7524/9/10/1154 |
| work_keys_str_mv | AT mansurhakanerol modernapproachestoinvitroclonalbananaproductionnextgenerationtissueculturesystems AT dicledonmez modernapproachestoinvitroclonalbananaproductionnextgenerationtissueculturesystems AT belginbicen modernapproachestoinvitroclonalbananaproductionnextgenerationtissueculturesystems AT ozhansimsek modernapproachestoinvitroclonalbananaproductionnextgenerationtissueculturesystems AT yıldızakakacar modernapproachestoinvitroclonalbananaproductionnextgenerationtissueculturesystems |