Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells
Objectives: Mycosynthesis offers a benevolent alternative for the synthesis of Silver Nanoparticles (AgNPs) when compared to the conventional physical and chemical methods. Fungi secrete enormous amounts of proteins and enzymes that act as reducing and capping agents in AgNPs synthesis. Methods: AgN...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722000635 |
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| author | S. Himalini V. Uma Maheshwari Nallal M. Razia Sasikala Chinnapan Murugesan Chandrasekaran Venkatalakshmi Ranganathan Mansour K. Gatasheh Ashraf Atef Hatamleh Fatimah S Al-Khattaf S. Kanimozhi |
| author_facet | S. Himalini V. Uma Maheshwari Nallal M. Razia Sasikala Chinnapan Murugesan Chandrasekaran Venkatalakshmi Ranganathan Mansour K. Gatasheh Ashraf Atef Hatamleh Fatimah S Al-Khattaf S. Kanimozhi |
| author_sort | S. Himalini |
| collection | DOAJ |
| description | Objectives: Mycosynthesis offers a benevolent alternative for the synthesis of Silver Nanoparticles (AgNPs) when compared to the conventional physical and chemical methods. Fungi secrete enormous amounts of proteins and enzymes that act as reducing and capping agents in AgNPs synthesis. Methods: AgNPs were synthesized using extracellular fungal extract of Fusarium incarnatum. The reaction mixture was optimized using different parameters to obtain better yield. Anti-melanogenic activity of fungal extract, AgNPs and standard drug (Kojic acid) was evaluated against Human skin melanoma SK-MEL-3 cells. AgNPs were synthesized at 30 °C, pH 5 and 1 mM AgNO3 concentration. Then optimization and the characterization were done by UV–Vis, FTIR, XRD, Zeta potential and H-TEM techniques. Results: The average size of AgNPs was 10 nm and predominantly spherical in shape. Myco-synthesized AgNPs showed significant anti-melanogenic activity against human skin melanoma SK-MEL-3 cells with an IC50 value of 17.70 μg/ml. The concentration of AgNPs required to inhibit SK-MEL-3 cells was lower than the concentrations of F. incarnatum extract and standard Kojic acid. Conclusion: Spherical AgNPs with and average size of 4 nm were synthesized using fungal extract of F. incarnatum. Mycosynthesized AgNPs can be considered as effective anti-melanogenic agents owing to their impressive activity against skin melanoma. |
| first_indexed | 2024-12-13T15:09:42Z |
| format | Article |
| id | doaj.art-b8a6db3466c146308da8a194e95ed940 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-13T15:09:42Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-b8a6db3466c146308da8a194e95ed9402022-12-21T23:40:55ZengElsevierJournal of King Saud University: Science1018-36472022-04-01343101882Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cellsS. Himalini0V. Uma Maheshwari Nallal1M. Razia2Sasikala Chinnapan3Murugesan Chandrasekaran4Venkatalakshmi Ranganathan5Mansour K. Gatasheh6Ashraf Atef Hatamleh7Fatimah S Al-Khattaf8S. Kanimozhi9Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal 624101, Tamilnadu, IndiaDepartment of Biotechnology, Mother Teresa Women’s University, Kodaikanal 624101, Tamilnadu, IndiaDepartment of Biotechnology, Mother Teresa Women’s University, Kodaikanal 624101, Tamilnadu, India; Corresponding authors.Department of Pharmaceutical Biology, Faculty of Pharmaceutical Science, UCSI University Kuala Lumpur, Kuala Lumpur, MalaysiaDepartment of Food Science and Biotechnology, Sejong University, Gwangjin-gu, Seoul 05006, Republic of Korea; Corresponding authors.Faculty of Pharmaceutical Sciences, UCSI University, 56000 Cheras, Kulala Lumpur, MalaysiaDepartment of Biochemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Biotechnology, Bharath Institute of Higher Education and Research, Selaiyur, Chennai, IndiaObjectives: Mycosynthesis offers a benevolent alternative for the synthesis of Silver Nanoparticles (AgNPs) when compared to the conventional physical and chemical methods. Fungi secrete enormous amounts of proteins and enzymes that act as reducing and capping agents in AgNPs synthesis. Methods: AgNPs were synthesized using extracellular fungal extract of Fusarium incarnatum. The reaction mixture was optimized using different parameters to obtain better yield. Anti-melanogenic activity of fungal extract, AgNPs and standard drug (Kojic acid) was evaluated against Human skin melanoma SK-MEL-3 cells. AgNPs were synthesized at 30 °C, pH 5 and 1 mM AgNO3 concentration. Then optimization and the characterization were done by UV–Vis, FTIR, XRD, Zeta potential and H-TEM techniques. Results: The average size of AgNPs was 10 nm and predominantly spherical in shape. Myco-synthesized AgNPs showed significant anti-melanogenic activity against human skin melanoma SK-MEL-3 cells with an IC50 value of 17.70 μg/ml. The concentration of AgNPs required to inhibit SK-MEL-3 cells was lower than the concentrations of F. incarnatum extract and standard Kojic acid. Conclusion: Spherical AgNPs with and average size of 4 nm were synthesized using fungal extract of F. incarnatum. Mycosynthesized AgNPs can be considered as effective anti-melanogenic agents owing to their impressive activity against skin melanoma.http://www.sciencedirect.com/science/article/pii/S1018364722000635MycosynthesisAgNPsResistanceKojic acidAnti-melanogenicSK-MEL-3 cells |
| spellingShingle | S. Himalini V. Uma Maheshwari Nallal M. Razia Sasikala Chinnapan Murugesan Chandrasekaran Venkatalakshmi Ranganathan Mansour K. Gatasheh Ashraf Atef Hatamleh Fatimah S Al-Khattaf S. Kanimozhi Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells Journal of King Saud University: Science Mycosynthesis AgNPs Resistance Kojic acid Anti-melanogenic SK-MEL-3 cells |
| title | Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells |
| title_full | Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells |
| title_fullStr | Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells |
| title_full_unstemmed | Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells |
| title_short | Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells |
| title_sort | antimicrobial anti melanogenesis and anti tyrosinase potential of myco synthesized silver nanoparticles on human skin melanoma sk mel 3 cells |
| topic | Mycosynthesis AgNPs Resistance Kojic acid Anti-melanogenic SK-MEL-3 cells |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722000635 |
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