DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method
Background: To determine the DNA content in subfertile patients and to correlate it with seminal sperm concentration. Design: Prospective observational study. Setting: College of Medicine, Dept. of Physiological chemistry and Institute for embryo Research and infertility treatment-University of Bagh...
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| Format: | Article |
| Language: | English |
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College of Medicine University of Baghdad
2007-01-01
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| Series: | مجلة كلية الطب |
| Subjects: | |
| Online Access: | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1467 |
| _version_ | 1827393793530265600 |
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| author | Munaf S. Daoud Faraid A. AL- chalabi |
| author_facet | Munaf S. Daoud Faraid A. AL- chalabi |
| author_sort | Munaf S. Daoud |
| collection | DOAJ |
| description | Background: To determine the DNA content in subfertile patients and to correlate it with seminal sperm concentration.
Design: Prospective observational study.
Setting: College of Medicine, Dept. of Physiological chemistry and Institute for embryo Research and infertility treatment-University of Baghdad.The study was conducted
through years 2004-2005.
Methods: A random sample of 61 subfertile male patients undergoing semen evaluation and aged from 20-45 years were studied. Semen samples were assessed for seminal sperm
concentration microscopically and were classified into 3 different groups according to count (million/ml).Then sperm D N A content (μg/ml) was estimated using a microchemical
spectrophotometric method.
Results: The three groups were statistically of significant difference( P <0.01 ).The D N A concentration per spermatozoan in Gr.III ( 1 20 million/ml) was higher than the other two
groups It was also noticed that there was a statistically significant (P< 0.05 ) correlation in Gr.III b etween D N A content and sperm concentration . No significant (P >0.05
)correlation was observed in the other two groups (Gr. I & Gr. II ).
Conclusion: Significant positive correlation was obtained between sperm concentration and DNA content. in the oligozoospermic subfertile patient , but no significant correlation was found in the normozoopermic patients . D N A content per spermatozoan from oligozoospermic patients was higher than that in normozoospermic patients. |
| first_indexed | 2024-03-08T17:56:33Z |
| format | Article |
| id | doaj.art-b8a84aa5b184478c822ab937015ee4c0 |
| institution | Directory Open Access Journal |
| issn | 0041-9419 2410-8057 |
| language | English |
| last_indexed | 2024-03-08T17:56:33Z |
| publishDate | 2007-01-01 |
| publisher | College of Medicine University of Baghdad |
| record_format | Article |
| series | مجلة كلية الطب |
| spelling | doaj.art-b8a84aa5b184478c822ab937015ee4c02024-01-02T04:56:52ZengCollege of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572007-01-01484DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric MethodMunaf S. Daoud0Faraid A. AL- chalabi1Department of Physiological Chemistry, College of Medicine, University of Baghdad.Department of Physiological Chemistry, College of Medicine, University of Baghdad.Background: To determine the DNA content in subfertile patients and to correlate it with seminal sperm concentration. Design: Prospective observational study. Setting: College of Medicine, Dept. of Physiological chemistry and Institute for embryo Research and infertility treatment-University of Baghdad.The study was conducted through years 2004-2005. Methods: A random sample of 61 subfertile male patients undergoing semen evaluation and aged from 20-45 years were studied. Semen samples were assessed for seminal sperm concentration microscopically and were classified into 3 different groups according to count (million/ml).Then sperm D N A content (μg/ml) was estimated using a microchemical spectrophotometric method. Results: The three groups were statistically of significant difference( P <0.01 ).The D N A concentration per spermatozoan in Gr.III ( 1 20 million/ml) was higher than the other two groups It was also noticed that there was a statistically significant (P< 0.05 ) correlation in Gr.III b etween D N A content and sperm concentration . No significant (P >0.05 )correlation was observed in the other two groups (Gr. I & Gr. II ). Conclusion: Significant positive correlation was obtained between sperm concentration and DNA content. in the oligozoospermic subfertile patient , but no significant correlation was found in the normozoopermic patients . D N A content per spermatozoan from oligozoospermic patients was higher than that in normozoospermic patients.http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1467DNA, Sperm concentration (count million/ml), Normozoospermic, Oligozoospermic. |
| spellingShingle | Munaf S. Daoud Faraid A. AL- chalabi DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method مجلة كلية الطب DNA, Sperm concentration (count million/ml), Normozoospermic, Oligozoospermic. |
| title | DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method |
| title_full | DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method |
| title_fullStr | DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method |
| title_full_unstemmed | DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method |
| title_short | DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method |
| title_sort | dna content of spermatozoa with respect to seminal sperm concentration using a microchemical spectrophotometric method |
| topic | DNA, Sperm concentration (count million/ml), Normozoospermic, Oligozoospermic. |
| url | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1467 |
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