Dietary phillygenin supplementation ameliorates aflatoxin B1-induced oxidative stress, inflammation, and apoptosis in chicken liver
Aflatoxin B1 (AFB1), a mycotoxin contaminating food and feed, can trigger liver immune toxicity and threaten the poultry industry. Phillygenin (PHI) is a natural lignan derived primarily from Forsythia suspensa with hepatoprotective pharmacological and medicinal properties. This research aimed to in...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322003219 |
| _version_ | 1818047096846024704 |
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| author | Jing Guo Wen-Rui Yan Jian-Kai Tang Xiang Jin Huan-Huan Xue Tao Wang Li-Wei Zhang Qian-Yun Sun Zhan-Xue Liang |
| author_facet | Jing Guo Wen-Rui Yan Jian-Kai Tang Xiang Jin Huan-Huan Xue Tao Wang Li-Wei Zhang Qian-Yun Sun Zhan-Xue Liang |
| author_sort | Jing Guo |
| collection | DOAJ |
| description | Aflatoxin B1 (AFB1), a mycotoxin contaminating food and feed, can trigger liver immune toxicity and threaten the poultry industry. Phillygenin (PHI) is a natural lignan derived primarily from Forsythia suspensa with hepatoprotective pharmacological and medicinal properties. This research aimed to investigate the preventive effects of PHI on the toxicity of AFB1 in the liver of chickens. Chickens were administered with AFB1 (2.8 mg/kg) and/or treated with PHI (24 mg/kg) for 33 days. The histopathological changes, serum biochemical indices, oxidative damage, inflammatory mediators, apoptosis, and activation of the NF-κB and Nrf2 signaling pathways were measured. Results revealed that dietary PHI ameliorated liver function indicators, reduced the malondialdehyde and inflammatory mediator production and the apoptotic cell number, and increased the antioxidant enzyme contents and Bcl-2 level. The quantitative realtime PCR and Western blot results revealed that PHI reduced p53, cytochrome c, Bax, caspase-9, and caspase-3 levels, normalized the NF-κB p65 phosphorylation, and upregulated the Nrf2 and its downstream genes expression in chicken liver. These results indicated that PHI has beneficial effects on AFB1-induced liver damage, oxidative damage, inflammatory response, apoptosis, and immunotoxicity by inhibiting NF-κB and activating the Nrf2 signaling pathway in chickens. This study provides new insight into the therapeutic uses of PHI. |
| first_indexed | 2024-12-10T10:00:22Z |
| format | Article |
| id | doaj.art-b8aa9aec4f7e4465a3ea0f73a5f6a48b |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-10T10:00:22Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-b8aa9aec4f7e4465a3ea0f73a5f6a48b2022-12-22T01:53:22ZengElsevierEcotoxicology and Environmental Safety0147-65132022-05-01236113481Dietary phillygenin supplementation ameliorates aflatoxin B1-induced oxidative stress, inflammation, and apoptosis in chicken liverJing Guo0Wen-Rui Yan1Jian-Kai Tang2Xiang Jin3Huan-Huan Xue4Tao Wang5Li-Wei Zhang6Qian-Yun Sun7Zhan-Xue Liang8Institute of Molecular Science, Mordern Research Center for Traditional Chinese Medicine, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China; State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, ChinaInstitute of Molecular Science, Mordern Research Center for Traditional Chinese Medicine, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, ChinaInstitute of Molecular Science, Mordern Research Center for Traditional Chinese Medicine, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, ChinaInstitute of Molecular Science, Mordern Research Center for Traditional Chinese Medicine, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, ChinaInstitute of Molecular Science, Mordern Research Center for Traditional Chinese Medicine, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, ChinaInstitute of Molecular Science, Mordern Research Center for Traditional Chinese Medicine, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, ChinaInstitute of Molecular Science, Mordern Research Center for Traditional Chinese Medicine, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China; Correspondance to: Institute of Molecular Science, Mordern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, ChinaState Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China; Center for Pharmacology and Bioactivity Research, The Key Laboratory of Chemistry for Natural Products, Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China; Corresponding author at: State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi, China; Corresponding author.Aflatoxin B1 (AFB1), a mycotoxin contaminating food and feed, can trigger liver immune toxicity and threaten the poultry industry. Phillygenin (PHI) is a natural lignan derived primarily from Forsythia suspensa with hepatoprotective pharmacological and medicinal properties. This research aimed to investigate the preventive effects of PHI on the toxicity of AFB1 in the liver of chickens. Chickens were administered with AFB1 (2.8 mg/kg) and/or treated with PHI (24 mg/kg) for 33 days. The histopathological changes, serum biochemical indices, oxidative damage, inflammatory mediators, apoptosis, and activation of the NF-κB and Nrf2 signaling pathways were measured. Results revealed that dietary PHI ameliorated liver function indicators, reduced the malondialdehyde and inflammatory mediator production and the apoptotic cell number, and increased the antioxidant enzyme contents and Bcl-2 level. The quantitative realtime PCR and Western blot results revealed that PHI reduced p53, cytochrome c, Bax, caspase-9, and caspase-3 levels, normalized the NF-κB p65 phosphorylation, and upregulated the Nrf2 and its downstream genes expression in chicken liver. These results indicated that PHI has beneficial effects on AFB1-induced liver damage, oxidative damage, inflammatory response, apoptosis, and immunotoxicity by inhibiting NF-κB and activating the Nrf2 signaling pathway in chickens. This study provides new insight into the therapeutic uses of PHI.http://www.sciencedirect.com/science/article/pii/S0147651322003219Aflatoxin BPhillygeninLiverAntioxidativeAnti-inflammatoryApoptosis |
| spellingShingle | Jing Guo Wen-Rui Yan Jian-Kai Tang Xiang Jin Huan-Huan Xue Tao Wang Li-Wei Zhang Qian-Yun Sun Zhan-Xue Liang Dietary phillygenin supplementation ameliorates aflatoxin B1-induced oxidative stress, inflammation, and apoptosis in chicken liver Ecotoxicology and Environmental Safety Aflatoxin B Phillygenin Liver Antioxidative Anti-inflammatory Apoptosis |
| title | Dietary phillygenin supplementation ameliorates aflatoxin B1-induced oxidative stress, inflammation, and apoptosis in chicken liver |
| title_full | Dietary phillygenin supplementation ameliorates aflatoxin B1-induced oxidative stress, inflammation, and apoptosis in chicken liver |
| title_fullStr | Dietary phillygenin supplementation ameliorates aflatoxin B1-induced oxidative stress, inflammation, and apoptosis in chicken liver |
| title_full_unstemmed | Dietary phillygenin supplementation ameliorates aflatoxin B1-induced oxidative stress, inflammation, and apoptosis in chicken liver |
| title_short | Dietary phillygenin supplementation ameliorates aflatoxin B1-induced oxidative stress, inflammation, and apoptosis in chicken liver |
| title_sort | dietary phillygenin supplementation ameliorates aflatoxin b1 induced oxidative stress inflammation and apoptosis in chicken liver |
| topic | Aflatoxin B Phillygenin Liver Antioxidative Anti-inflammatory Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322003219 |
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