The Impacts of Asparagus Extract Fractions on Growth and Fumonisins Biosynthesis in Fusarium Proliferatum
Asparagus is a genus consisting of over two hundred species of perennial plants. <i>Fusarium </i><i>proliferatum</i><i> </i>is a major asparagus pathogen and it<i> </i>biosynthesizes a variety of mycotoxins, of which fumonisins B are prevalent. Our pre...
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MDPI AG
2020-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/2/95 |
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| author | Natalia Witaszak Justyna Lalak-Kańczugowska Agnieszka Waśkiewicz Łukasz Stępień |
| author_facet | Natalia Witaszak Justyna Lalak-Kańczugowska Agnieszka Waśkiewicz Łukasz Stępień |
| author_sort | Natalia Witaszak |
| collection | DOAJ |
| description | Asparagus is a genus consisting of over two hundred species of perennial plants. <i>Fusarium </i><i>proliferatum</i><i> </i>is a major asparagus pathogen and it<i> </i>biosynthesizes a variety of mycotoxins, of which fumonisins B are prevalent. Our previous studies on <i>F. </i><i>proliferatum</i> strains indicated that asparagus extract affects the expression of <i>FUM1</i> gene, encoding polyketide synthase, a key enzyme of the <i>FUM</i> gene cluster governing the biosynthesis of fumonisins. An asparagus-derived <i>F. </i><i>proliferatum</i> strain increased fumonisin B<sub>1 </sub>production after extract fractions’ addition, reaching the maximum 2 or 24 h after treatment. The cultures yielded between 40 and 520 mg of dry weight of mycelia after 14 days of cultivation. The differences in fungal biomass amounts between the whole extract and its fractions may result from synergistic effect of all bioactive compounds present in asparagus extract. Among extract fractions, the methanolic fraction had the highest effect on the dry weight of the mycelium reaching about a 13-fold increase compared to the control. Furthermore, we measured the relative expression of the <i>FUM1 </i>gene. Due to the possible antifungal activity of tested extract fractions, future research will be focused on the identification of the <i>Asparagus officinalis</i> L. compounds responsible for this activity. |
| first_indexed | 2024-04-11T12:54:21Z |
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| language | English |
| last_indexed | 2024-04-11T12:54:21Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b8ac5ed3208b4871b3c4d6402a23c6782022-12-22T04:23:07ZengMDPI AGToxins2072-66512020-01-011229510.3390/toxins12020095toxins12020095The Impacts of Asparagus Extract Fractions on Growth and Fumonisins Biosynthesis in Fusarium ProliferatumNatalia Witaszak0Justyna Lalak-Kańczugowska1Agnieszka Waśkiewicz2Łukasz Stępień3Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandDepartment of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandDepartment of Chemistry, Poznan University of Life Sciences, Wojska Polskiego 75, 60-637 Poznań, PolandDepartment of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandAsparagus is a genus consisting of over two hundred species of perennial plants. <i>Fusarium </i><i>proliferatum</i><i> </i>is a major asparagus pathogen and it<i> </i>biosynthesizes a variety of mycotoxins, of which fumonisins B are prevalent. Our previous studies on <i>F. </i><i>proliferatum</i> strains indicated that asparagus extract affects the expression of <i>FUM1</i> gene, encoding polyketide synthase, a key enzyme of the <i>FUM</i> gene cluster governing the biosynthesis of fumonisins. An asparagus-derived <i>F. </i><i>proliferatum</i> strain increased fumonisin B<sub>1 </sub>production after extract fractions’ addition, reaching the maximum 2 or 24 h after treatment. The cultures yielded between 40 and 520 mg of dry weight of mycelia after 14 days of cultivation. The differences in fungal biomass amounts between the whole extract and its fractions may result from synergistic effect of all bioactive compounds present in asparagus extract. Among extract fractions, the methanolic fraction had the highest effect on the dry weight of the mycelium reaching about a 13-fold increase compared to the control. Furthermore, we measured the relative expression of the <i>FUM1 </i>gene. Due to the possible antifungal activity of tested extract fractions, future research will be focused on the identification of the <i>Asparagus officinalis</i> L. compounds responsible for this activity.https://www.mdpi.com/2072-6651/12/2/95asparagus<i>fusarium proliferatum</i>fumonisinsplant-pathogen interactionqpcruplc/tqd |
| spellingShingle | Natalia Witaszak Justyna Lalak-Kańczugowska Agnieszka Waśkiewicz Łukasz Stępień The Impacts of Asparagus Extract Fractions on Growth and Fumonisins Biosynthesis in Fusarium Proliferatum Toxins asparagus <i>fusarium proliferatum</i> fumonisins plant-pathogen interaction qpcr uplc/tqd |
| title | The Impacts of Asparagus Extract Fractions on Growth and Fumonisins Biosynthesis in Fusarium Proliferatum |
| title_full | The Impacts of Asparagus Extract Fractions on Growth and Fumonisins Biosynthesis in Fusarium Proliferatum |
| title_fullStr | The Impacts of Asparagus Extract Fractions on Growth and Fumonisins Biosynthesis in Fusarium Proliferatum |
| title_full_unstemmed | The Impacts of Asparagus Extract Fractions on Growth and Fumonisins Biosynthesis in Fusarium Proliferatum |
| title_short | The Impacts of Asparagus Extract Fractions on Growth and Fumonisins Biosynthesis in Fusarium Proliferatum |
| title_sort | impacts of asparagus extract fractions on growth and fumonisins biosynthesis in fusarium proliferatum |
| topic | asparagus <i>fusarium proliferatum</i> fumonisins plant-pathogen interaction qpcr uplc/tqd |
| url | https://www.mdpi.com/2072-6651/12/2/95 |
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