Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts
Abstract Background Lignocellulosic resources are promising feedstocks for the manufacture of bio-based products and bioenergy. However, the inherent recalcitrance of biomass to conversion into simple sugars currently hinders the deployment of advanced bioproducts at large scale. Lignin is a primary...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-022-02245-4 |
| _version_ | 1797977559547248640 |
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| author | Chien-Yuan Lin Gina M. Geiselman Di Liu Harsha D. Magurudeniya Alberto Rodriguez Yi-Chun Chen Venkataramana Pidatala Faride Unda Bashar Amer Edward E. K. Baidoo Shawn D. Mansfield Blake A. Simmons Seema Singh Henrik V. Scheller John M. Gladden Aymerick Eudes |
| author_facet | Chien-Yuan Lin Gina M. Geiselman Di Liu Harsha D. Magurudeniya Alberto Rodriguez Yi-Chun Chen Venkataramana Pidatala Faride Unda Bashar Amer Edward E. K. Baidoo Shawn D. Mansfield Blake A. Simmons Seema Singh Henrik V. Scheller John M. Gladden Aymerick Eudes |
| author_sort | Chien-Yuan Lin |
| collection | DOAJ |
| description | Abstract Background Lignocellulosic resources are promising feedstocks for the manufacture of bio-based products and bioenergy. However, the inherent recalcitrance of biomass to conversion into simple sugars currently hinders the deployment of advanced bioproducts at large scale. Lignin is a primary contributor to biomass recalcitrance as it protects cell wall polysaccharides from degradation and can inhibit hydrolytic enzymes via non-productive adsorption. Several engineering strategies have been designed to reduce lignin or modify its monomeric composition. For example, expression of bacterial 3-dehydroshikimate dehydratase (QsuB) in poplar trees resulted in a reduction in lignin due to redirection of metabolic flux toward 3,4-dihydroxybenzoate at the expense of lignin. This reduction was accompanied with remarkable changes in the pools of aromatic compounds that accumulate in the biomass. Results The impact of these modifications on downstream biomass deconstruction and conversion into advanced bioproducts was evaluated in the current study. Using ionic liquid pretreatment followed by enzymatic saccharification, biomass from engineered trees released more glucose and xylose compared to wild-type control trees under optimum conditions. Fermentation of the resulting hydrolysates using Rhodosporidium toruloides strains engineered to produce α-bisabolene, epi-isozizaene, and fatty alcohols showed no negative impact on cell growth and yielded higher titers of bioproducts (as much as + 58%) in the case of QsuB transgenics trees. Conclusion Our data show that low-recalcitrant poplar biomass obtained with the QsuB technology has the potential to improve the production of advanced bioproducts. |
| first_indexed | 2024-04-11T05:08:53Z |
| format | Article |
| id | doaj.art-b8af2916429e499685b3d9e637da949e |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-11T05:08:53Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-b8af2916429e499685b3d9e637da949e2022-12-25T12:08:04ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542022-12-0115111210.1186/s13068-022-02245-4Evaluation of engineered low-lignin poplar for conversion into advanced bioproductsChien-Yuan Lin0Gina M. Geiselman1Di Liu2Harsha D. Magurudeniya3Alberto Rodriguez4Yi-Chun Chen5Venkataramana Pidatala6Faride Unda7Bashar Amer8Edward E. K. Baidoo9Shawn D. Mansfield10Blake A. Simmons11Seema Singh12Henrik V. Scheller13John M. Gladden14Aymerick Eudes15DOE Joint BioEnergy InstituteDOE Joint BioEnergy InstituteDepartment of Biomaterials and Biomanufacturing, Sandia National LaboratoriesDOE Joint BioEnergy InstituteDOE Joint BioEnergy InstituteDOE Joint BioEnergy InstituteDOE Joint BioEnergy InstituteDepartment of Wood Science, University of British ColumbiaDOE Joint BioEnergy InstituteDOE Joint BioEnergy InstituteDepartment of Wood Science, University of British ColumbiaDOE Joint BioEnergy InstituteDOE Joint BioEnergy InstituteDOE Joint BioEnergy InstituteDOE Joint BioEnergy InstituteDOE Joint BioEnergy InstituteAbstract Background Lignocellulosic resources are promising feedstocks for the manufacture of bio-based products and bioenergy. However, the inherent recalcitrance of biomass to conversion into simple sugars currently hinders the deployment of advanced bioproducts at large scale. Lignin is a primary contributor to biomass recalcitrance as it protects cell wall polysaccharides from degradation and can inhibit hydrolytic enzymes via non-productive adsorption. Several engineering strategies have been designed to reduce lignin or modify its monomeric composition. For example, expression of bacterial 3-dehydroshikimate dehydratase (QsuB) in poplar trees resulted in a reduction in lignin due to redirection of metabolic flux toward 3,4-dihydroxybenzoate at the expense of lignin. This reduction was accompanied with remarkable changes in the pools of aromatic compounds that accumulate in the biomass. Results The impact of these modifications on downstream biomass deconstruction and conversion into advanced bioproducts was evaluated in the current study. Using ionic liquid pretreatment followed by enzymatic saccharification, biomass from engineered trees released more glucose and xylose compared to wild-type control trees under optimum conditions. Fermentation of the resulting hydrolysates using Rhodosporidium toruloides strains engineered to produce α-bisabolene, epi-isozizaene, and fatty alcohols showed no negative impact on cell growth and yielded higher titers of bioproducts (as much as + 58%) in the case of QsuB transgenics trees. Conclusion Our data show that low-recalcitrant poplar biomass obtained with the QsuB technology has the potential to improve the production of advanced bioproducts.https://doi.org/10.1186/s13068-022-02245-4Woody biomassRhodosporidium toruloidesIonic liquidSaccharificationAromaticsFermentation |
| spellingShingle | Chien-Yuan Lin Gina M. Geiselman Di Liu Harsha D. Magurudeniya Alberto Rodriguez Yi-Chun Chen Venkataramana Pidatala Faride Unda Bashar Amer Edward E. K. Baidoo Shawn D. Mansfield Blake A. Simmons Seema Singh Henrik V. Scheller John M. Gladden Aymerick Eudes Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts Biotechnology for Biofuels and Bioproducts Woody biomass Rhodosporidium toruloides Ionic liquid Saccharification Aromatics Fermentation |
| title | Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts |
| title_full | Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts |
| title_fullStr | Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts |
| title_full_unstemmed | Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts |
| title_short | Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts |
| title_sort | evaluation of engineered low lignin poplar for conversion into advanced bioproducts |
| topic | Woody biomass Rhodosporidium toruloides Ionic liquid Saccharification Aromatics Fermentation |
| url | https://doi.org/10.1186/s13068-022-02245-4 |
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