Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites
Multidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polym...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12519 |
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| author | William M. Facchinatto Leandro O. Araujo Tiago B. Moraes Thais F. Abelha Thalita H. N. Lima Danilo M. dos Santos Sérgio P. Campana-Filho Luiz A. Colnago Anderson R. L. Caires |
| author_facet | William M. Facchinatto Leandro O. Araujo Tiago B. Moraes Thais F. Abelha Thalita H. N. Lima Danilo M. dos Santos Sérgio P. Campana-Filho Luiz A. Colnago Anderson R. L. Caires |
| author_sort | William M. Facchinatto |
| collection | DOAJ |
| description | Multidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(<i>p</i>-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (<i>E. coli</i>) and Gram-positive (<i>S. aureus</i>) bacteria. Chitosan’s structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species (ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxation time T<sub>2</sub> confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similar and shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect under blue-light irradiation. Overall, our findings indicate that chitosan/CNPPV NCPs may constitute a valuable asset for the development of innovative strategies for inactivation and/or photoinactivation of bacteria. |
| first_indexed | 2024-03-09T20:06:09Z |
| format | Article |
| id | doaj.art-b8b525f14c0d44ca82597440cda7b5b4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:06:09Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b8b525f14c0d44ca82597440cda7b5b42023-11-24T00:33:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201251910.3390/ijms232012519Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV NanocompositesWilliam M. Facchinatto0Leandro O. Araujo1Tiago B. Moraes2Thais F. Abelha3Thalita H. N. Lima4Danilo M. dos Santos5Sérgio P. Campana-Filho6Luiz A. Colnago7Anderson R. L. Caires8Laboratory of Optics and Photonics, Institute of Physics, Federal University of Mato Grosso do Sul, Ave. Costa e Silva s/n, Campo Grande 79070-900, MS, BrazilLaboratory of Optics and Photonics, Institute of Physics, Federal University of Mato Grosso do Sul, Ave. Costa e Silva s/n, Campo Grande 79070-900, MS, BrazilDepartment of Biosystems Engineering, “Luiz de Queiroz” College of Agriculture, University of São Paulo, Ave. Pádua Dias 11, Piracicaba 13418-900, SP, BrazilLaboratory of Optics and Photonics, Institute of Physics, Federal University of Mato Grosso do Sul, Ave. Costa e Silva s/n, Campo Grande 79070-900, MS, BrazilLaboratory of Optics and Photonics, Institute of Physics, Federal University of Mato Grosso do Sul, Ave. Costa e Silva s/n, Campo Grande 79070-900, MS, BrazilBrazilian Corporation for Agricultural Research, Embrapa Instrumentation, St. XV de Novembro 1452, São Carlos 13560-970, SP, BrazilSão Carlos Institute of Chemistry, University of São Paulo, Ave. Trabalhador São-Carlense 400, São Carlos 13560-590, SP, BrazilBrazilian Corporation for Agricultural Research, Embrapa Instrumentation, St. XV de Novembro 1452, São Carlos 13560-970, SP, BrazilLaboratory of Optics and Photonics, Institute of Physics, Federal University of Mato Grosso do Sul, Ave. Costa e Silva s/n, Campo Grande 79070-900, MS, BrazilMultidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(<i>p</i>-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (<i>E. coli</i>) and Gram-positive (<i>S. aureus</i>) bacteria. Chitosan’s structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species (ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxation time T<sub>2</sub> confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similar and shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect under blue-light irradiation. Overall, our findings indicate that chitosan/CNPPV NCPs may constitute a valuable asset for the development of innovative strategies for inactivation and/or photoinactivation of bacteria.https://www.mdpi.com/1422-0067/23/20/12519photoantimicrobial activityblue-light irradiationchitosanCNPPVnanocomposites<i>E. coli</i> |
| spellingShingle | William M. Facchinatto Leandro O. Araujo Tiago B. Moraes Thais F. Abelha Thalita H. N. Lima Danilo M. dos Santos Sérgio P. Campana-Filho Luiz A. Colnago Anderson R. L. Caires Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites International Journal of Molecular Sciences photoantimicrobial activity blue-light irradiation chitosan CNPPV nanocomposites <i>E. coli</i> |
| title | Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites |
| title_full | Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites |
| title_fullStr | Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites |
| title_full_unstemmed | Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites |
| title_short | Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites |
| title_sort | antimicrobial and photoantimicrobial activities of chitosan cnppv nanocomposites |
| topic | photoantimicrobial activity blue-light irradiation chitosan CNPPV nanocomposites <i>E. coli</i> |
| url | https://www.mdpi.com/1422-0067/23/20/12519 |
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