In vitro antioxidant activity‐guided fractionation of Daucus carota L. seed extract
Daucus carota Linn (carrot) seeds are medicinally useful in the management of diseases including diabetes mellitus. The present study investigates the in vitro antioxidant activities and phytochemical constituents of several fractions from aqueous seed extract of Daucus carota. D. carota seeds (78.8...
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| Format: | Article |
| Language: | English |
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Nigerian Academy of Science
2020-12-01
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| Series: | The Proceedings of the Nigerian Academy of Science |
| Subjects: | |
| Online Access: | https://nasjournal.org.ng/site/index.php/pnas/article/view/279/147 |
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| author | Habibu Tijjani Abubakar Mohammed Farida Abdullahi Ahmed Hindatu Bala Yahaya Nyarhm Zakka |
| author_facet | Habibu Tijjani Abubakar Mohammed Farida Abdullahi Ahmed Hindatu Bala Yahaya Nyarhm Zakka |
| author_sort | Habibu Tijjani |
| collection | DOAJ |
| description | Daucus carota Linn (carrot) seeds are medicinally useful in the management of diseases including diabetes mellitus. The present study investigates the in vitro antioxidant activities and phytochemical constituents of several fractions from aqueous seed extract of Daucus carota. D. carota seeds (78.8g) were pulverized and dissolved in 400 mL of distilled water for 24 hours. The crude extract obtained (16.4g, 20.8% yield) was partitioned in water/ethyl acetate (3:1) to yield ethyl acetate fraction (6.2g, 37.8% yield) and aqueous ethyl acetate fraction (7.6g, 46.3% yield) which was subjected to column chromatography. Thirteen (13) fractions obtained were evaluated for their in vitro antioxidant activities and screened for phytochemical constituents. The fractions exhibited in vitro antioxidant activities at 2 – 20 μg/mL with 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities (IC50 = 1.38 - 22.96 μg/mL), total antioxidant capacity (IC50 = 1.17 - 3.13 μg/mL) and nitric oxide scavenging activities (IC50 = 3.20 - 20.72 μg/mL). Fractions 4 – 8 expressed promising in vitro hydrogen peroxide (H2O2) antioxidant activities with IC50 comparable with that of ascorbic acid and butylated hydroxytoluene. The phytochemical screening of fractions 4 – 8 revealed the presence of alkaloids, flavonoids, and phenolics, which could be responsible for the antioxidant activities. The results suggest that fractions obtained from Daucus carota L. seed extracts possess significant antioxidant potential. |
| first_indexed | 2024-03-13T05:51:11Z |
| format | Article |
| id | doaj.art-b8b700a2ccf448e0a46270bc7bcfb20a |
| institution | Directory Open Access Journal |
| issn | 0794-7976 2705-327X |
| language | English |
| last_indexed | 2024-03-13T05:51:11Z |
| publishDate | 2020-12-01 |
| publisher | Nigerian Academy of Science |
| record_format | Article |
| series | The Proceedings of the Nigerian Academy of Science |
| spelling | doaj.art-b8b700a2ccf448e0a46270bc7bcfb20a2023-06-13T14:10:11ZengNigerian Academy of ScienceThe Proceedings of the Nigerian Academy of Science0794-79762705-327X2020-12-011327485https://doi.org/10.57046/WRHB6277In vitro antioxidant activity‐guided fractionation of Daucus carota L. seed extractHabibu Tijjani0Abubakar Mohammed1Farida Abdullahi Ahmed2Hindatu Bala Yahaya3Nyarhm Zakka4Department of Biochemistry, Natural Product Research Laboratory, Bauchi State University, Gadau, Bauchi State, Nigeria.Department of Biochemistry, Natural Product Research Laboratory, Bauchi State University, Gadau, Bauchi State, Nigeria & Department of Biochemistry, Federal University Lafia, Lafia, Nasarawa State, NigeriaDepartment of Biochemistry, Natural Product Research Laboratory, Bauchi State University, Gadau, Bauchi State, NigeriaDepartment of Biochemistry, Natural Product Research Laboratory, Bauchi State University, Gadau, Bauchi State, NigeriaDepartment of Biochemistry, Natural Product Research Laboratory, Bauchi State University, Gadau, Bauchi State, Nigeria.Daucus carota Linn (carrot) seeds are medicinally useful in the management of diseases including diabetes mellitus. The present study investigates the in vitro antioxidant activities and phytochemical constituents of several fractions from aqueous seed extract of Daucus carota. D. carota seeds (78.8g) were pulverized and dissolved in 400 mL of distilled water for 24 hours. The crude extract obtained (16.4g, 20.8% yield) was partitioned in water/ethyl acetate (3:1) to yield ethyl acetate fraction (6.2g, 37.8% yield) and aqueous ethyl acetate fraction (7.6g, 46.3% yield) which was subjected to column chromatography. Thirteen (13) fractions obtained were evaluated for their in vitro antioxidant activities and screened for phytochemical constituents. The fractions exhibited in vitro antioxidant activities at 2 – 20 μg/mL with 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities (IC50 = 1.38 - 22.96 μg/mL), total antioxidant capacity (IC50 = 1.17 - 3.13 μg/mL) and nitric oxide scavenging activities (IC50 = 3.20 - 20.72 μg/mL). Fractions 4 – 8 expressed promising in vitro hydrogen peroxide (H2O2) antioxidant activities with IC50 comparable with that of ascorbic acid and butylated hydroxytoluene. The phytochemical screening of fractions 4 – 8 revealed the presence of alkaloids, flavonoids, and phenolics, which could be responsible for the antioxidant activities. The results suggest that fractions obtained from Daucus carota L. seed extracts possess significant antioxidant potential.https://nasjournal.org.ng/site/index.php/pnas/article/view/279/147in vitroantioxidantsdaucus carotafractionationseeds |
| spellingShingle | Habibu Tijjani Abubakar Mohammed Farida Abdullahi Ahmed Hindatu Bala Yahaya Nyarhm Zakka In vitro antioxidant activity‐guided fractionation of Daucus carota L. seed extract The Proceedings of the Nigerian Academy of Science in vitro antioxidants daucus carota fractionation seeds |
| title | In vitro antioxidant activity‐guided fractionation of Daucus carota L. seed extract |
| title_full | In vitro antioxidant activity‐guided fractionation of Daucus carota L. seed extract |
| title_fullStr | In vitro antioxidant activity‐guided fractionation of Daucus carota L. seed extract |
| title_full_unstemmed | In vitro antioxidant activity‐guided fractionation of Daucus carota L. seed extract |
| title_short | In vitro antioxidant activity‐guided fractionation of Daucus carota L. seed extract |
| title_sort | in vitro antioxidant activity guided fractionation of daucus carota l seed extract |
| topic | in vitro antioxidants daucus carota fractionation seeds |
| url | https://nasjournal.org.ng/site/index.php/pnas/article/view/279/147 |
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