Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork [version 2; peer review: 2 approved]
Commercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing “Microscopi”, an accessible, affordable, DIY automated imaging system that is bui...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wellcome
2022-03-01
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| Series: | Wellcome Open Research |
| Subjects: | |
| Online Access: | https://wellcomeopenresearch.org/articles/6-63/v2 |
| _version_ | 1818008956812918784 |
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| author | Andrew Jefferson Matthew Wincott Martin J. Booth Ian M. Dobbie Richard M. Parton Ilan Davis |
| author_facet | Andrew Jefferson Matthew Wincott Martin J. Booth Ian M. Dobbie Richard M. Parton Ilan Davis |
| author_sort | Andrew Jefferson |
| collection | DOAJ |
| description | Commercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing “Microscopi”, an accessible, affordable, DIY automated imaging system that is built from 3D printed and commodity off-the-shelf hardware, including electro-mechanical, computer and optical components. Our design features automated sample navigation and image capture with a simple web-based graphical user interface, accessible with a tablet or other mobile device. The light path can easily be switched between different imaging modalities. The open source Python-based control software allows the hardware to be driven as an integrated imaging system. Furthermore, the microscope is fully customisable, which also enhances its value as a learning tool. Here, we describe the basic design and demonstrate imaging performance for a range of easily sourced specimens. |
| first_indexed | 2024-04-14T05:36:55Z |
| format | Article |
| id | doaj.art-b8b9858f54d04096a77cd4b209a736f3 |
| institution | Directory Open Access Journal |
| issn | 2398-502X |
| language | English |
| last_indexed | 2024-04-14T05:36:55Z |
| publishDate | 2022-03-01 |
| publisher | Wellcome |
| record_format | Article |
| series | Wellcome Open Research |
| spelling | doaj.art-b8b9858f54d04096a77cd4b209a736f32022-12-22T02:09:37ZengWellcomeWellcome Open Research2398-502X2022-03-01619702Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork [version 2; peer review: 2 approved]Andrew Jefferson0Matthew Wincott1Martin J. Booth2https://orcid.org/0000-0002-9525-8981Ian M. Dobbie3https://orcid.org/0000-0002-5531-5865Richard M. Parton4https://orcid.org/0000-0002-2152-4271Ilan Davis5Dementia Research Institute, Cardiff University, Cardiff, CF24 4HQ, UK, UKDepartment of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UKDepartment of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UKMicron Advanced Bio-imaging Unit, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UKMicron Advanced Bio-imaging Unit, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UKMicron Advanced Bio-imaging Unit, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UKCommercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing “Microscopi”, an accessible, affordable, DIY automated imaging system that is built from 3D printed and commodity off-the-shelf hardware, including electro-mechanical, computer and optical components. Our design features automated sample navigation and image capture with a simple web-based graphical user interface, accessible with a tablet or other mobile device. The light path can easily be switched between different imaging modalities. The open source Python-based control software allows the hardware to be driven as an integrated imaging system. Furthermore, the microscope is fully customisable, which also enhances its value as a learning tool. Here, we describe the basic design and demonstrate imaging performance for a range of easily sourced specimens.https://wellcomeopenresearch.org/articles/6-63/v2Microscopy Democratised technology open source Python programming. STEM lessoneng |
| spellingShingle | Andrew Jefferson Matthew Wincott Martin J. Booth Ian M. Dobbie Richard M. Parton Ilan Davis Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork [version 2; peer review: 2 approved] Wellcome Open Research Microscopy Democratised technology open source Python programming. STEM lesson eng |
| title | Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork [version 2; peer review: 2 approved] |
| title_full | Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork [version 2; peer review: 2 approved] |
| title_fullStr | Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork [version 2; peer review: 2 approved] |
| title_full_unstemmed | Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork [version 2; peer review: 2 approved] |
| title_short | Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork [version 2; peer review: 2 approved] |
| title_sort | democratising microscopi a 3d printed automated xyzt fluorescence imaging system for teaching outreach and fieldwork version 2 peer review 2 approved |
| topic | Microscopy Democratised technology open source Python programming. STEM lesson eng |
| url | https://wellcomeopenresearch.org/articles/6-63/v2 |
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