Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer Therapy
Late diagnosis and systemic toxicity associated with conventional treatments make oncological therapy significantly difficult. In this context, nanomedicine emerges as a new approach in the prevention, diagnosis and treatment of cancer. In this work, pH-sensitive solid magnetoliposomes (SMLs) were d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/6/1051 |
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| author | Ana Rita F. Pacheco Beatriz D. Cardoso Ana Pires André M. Pereira João P. Araújo Violeta M. Carvalho Raquel O. Rodrigues Paulo J. G. Coutinho Teresa Castelo-Grande Paulo A. Augusto Domingos Barbosa Rui A. Lima Senhorinha F. C. F. Teixeira Ana Rita O. Rodrigues Elisabete M. S. Castanheira |
| author_facet | Ana Rita F. Pacheco Beatriz D. Cardoso Ana Pires André M. Pereira João P. Araújo Violeta M. Carvalho Raquel O. Rodrigues Paulo J. G. Coutinho Teresa Castelo-Grande Paulo A. Augusto Domingos Barbosa Rui A. Lima Senhorinha F. C. F. Teixeira Ana Rita O. Rodrigues Elisabete M. S. Castanheira |
| author_sort | Ana Rita F. Pacheco |
| collection | DOAJ |
| description | Late diagnosis and systemic toxicity associated with conventional treatments make oncological therapy significantly difficult. In this context, nanomedicine emerges as a new approach in the prevention, diagnosis and treatment of cancer. In this work, pH-sensitive solid magnetoliposomes (SMLs) were developed for controlled release of the chemotherapeutic drug doxorubicin (DOX). Shape anisotropic magnetic nanoparticles of magnesium ferrite with partial substitution by calcium (Mg<sub>0.75</sub>Ca<sub>0.25</sub>Fe<sub>2</sub>O<sub>4</sub>) were synthesized, with and without calcination, and their structural, morphological and magnetic properties were investigated. Their superparamagnetic properties were evaluated and heating capabilities proven, either by exposure to an alternating magnetic field (AMF) (magnetic hyperthermia) or by irradiation with near-infrared (NIR) light (photothermia). The Mg<sub>0.75</sub>Ca<sub>0.25</sub>Fe<sub>2</sub>O<sub>4</sub> calcined nanoparticles were selected to integrate the SMLs, surrounded by a lipid bilayer of DOPE:Ch:CHEMS (45:45:10). DOX was encapsulated in the nanosystems with an efficiency above 98%. DOX release assays showed a much more efficient release of the drug at pH = 5 compared to the release kinetics at physiological pH. By subjecting tumor cells to DOX-loaded SMLs, cell viability was significantly reduced, confirming that they can release the encapsulated drug. These results point to the development of efficient pH-sensitive nanocarriers, suitable for a synergistic action in cancer therapy with magnetic targeting, stimulus-controlled drug delivery and dual hyperthermia (magnetic and plasmonic) therapy. |
| first_indexed | 2024-03-11T06:05:26Z |
| format | Article |
| id | doaj.art-b8bcb28fd00641068bd96580f18d6ec2 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T06:05:26Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-b8bcb28fd00641068bd96580f18d6ec22023-11-17T13:00:51ZengMDPI AGNanomaterials2079-49912023-03-01136105110.3390/nano13061051Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer TherapyAna Rita F. Pacheco0Beatriz D. Cardoso1Ana Pires2André M. Pereira3João P. Araújo4Violeta M. Carvalho5Raquel O. Rodrigues6Paulo J. G. Coutinho7Teresa Castelo-Grande8Paulo A. Augusto9Domingos Barbosa10Rui A. Lima11Senhorinha F. C. F. Teixeira12Ana Rita O. Rodrigues13Elisabete M. S. Castanheira14Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalPhysics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalAssociate Laboratory LaPMET, 4169-007 Porto, PortugalAssociate Laboratory LaPMET, 4169-007 Porto, PortugalAssociate Laboratory LaPMET, 4169-007 Porto, PortugalMEtRICs, Mechanical Engineering Department, University of Minho, Campus de Azurém, 4800-058 Guimarães, PortugalCenter for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, Campus de Azurém, 4800-058 Guimarães, PortugalPhysics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalLEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalMEtRICs, Mechanical Engineering Department, University of Minho, Campus de Azurém, 4800-058 Guimarães, PortugalALGORITMI Center, University of Minho, Campus de Azurém, 4800-058 Guimarães, PortugalPhysics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalPhysics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalLate diagnosis and systemic toxicity associated with conventional treatments make oncological therapy significantly difficult. In this context, nanomedicine emerges as a new approach in the prevention, diagnosis and treatment of cancer. In this work, pH-sensitive solid magnetoliposomes (SMLs) were developed for controlled release of the chemotherapeutic drug doxorubicin (DOX). Shape anisotropic magnetic nanoparticles of magnesium ferrite with partial substitution by calcium (Mg<sub>0.75</sub>Ca<sub>0.25</sub>Fe<sub>2</sub>O<sub>4</sub>) were synthesized, with and without calcination, and their structural, morphological and magnetic properties were investigated. Their superparamagnetic properties were evaluated and heating capabilities proven, either by exposure to an alternating magnetic field (AMF) (magnetic hyperthermia) or by irradiation with near-infrared (NIR) light (photothermia). The Mg<sub>0.75</sub>Ca<sub>0.25</sub>Fe<sub>2</sub>O<sub>4</sub> calcined nanoparticles were selected to integrate the SMLs, surrounded by a lipid bilayer of DOPE:Ch:CHEMS (45:45:10). DOX was encapsulated in the nanosystems with an efficiency above 98%. DOX release assays showed a much more efficient release of the drug at pH = 5 compared to the release kinetics at physiological pH. By subjecting tumor cells to DOX-loaded SMLs, cell viability was significantly reduced, confirming that they can release the encapsulated drug. These results point to the development of efficient pH-sensitive nanocarriers, suitable for a synergistic action in cancer therapy with magnetic targeting, stimulus-controlled drug delivery and dual hyperthermia (magnetic and plasmonic) therapy.https://www.mdpi.com/2079-4991/13/6/1051combined cancer therapydrug releasepH-sensitive magnetoliposomesshape anisotropic nanoparticlessuperparamagnetism |
| spellingShingle | Ana Rita F. Pacheco Beatriz D. Cardoso Ana Pires André M. Pereira João P. Araújo Violeta M. Carvalho Raquel O. Rodrigues Paulo J. G. Coutinho Teresa Castelo-Grande Paulo A. Augusto Domingos Barbosa Rui A. Lima Senhorinha F. C. F. Teixeira Ana Rita O. Rodrigues Elisabete M. S. Castanheira Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer Therapy Nanomaterials combined cancer therapy drug release pH-sensitive magnetoliposomes shape anisotropic nanoparticles superparamagnetism |
| title | Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer Therapy |
| title_full | Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer Therapy |
| title_fullStr | Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer Therapy |
| title_full_unstemmed | Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer Therapy |
| title_short | Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer Therapy |
| title_sort | development of ph sensitive magnetoliposomes containing shape anisotropic nanoparticles for potential application in combined cancer therapy |
| topic | combined cancer therapy drug release pH-sensitive magnetoliposomes shape anisotropic nanoparticles superparamagnetism |
| url | https://www.mdpi.com/2079-4991/13/6/1051 |
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