Prevalence of Class 2 Integrons in Multidrug-Resistant Acinetobacter Baumannii in Toxicological ICU Patients in Tehran
Background: Acinetobacter baumannii is an important opportunistic pathogen which causes complications in hospitalized patients, especially those in ICU. The aim of this study was to determine the frequency of class 1 and 2 integrons in multi-drug resistance A. baumannii and to investigate the associ...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2016-07-01
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| Series: | Iranian Journal of Toxicology |
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| Online Access: | http://ijt.arakmu.ac.ir/browse.php?a_id=258&sid=1&slc_lang=en |
| _version_ | 1811300522966122496 |
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| author | Sepideh Kamalbeik Mehran Kouchek Majid Baseri Salehi Fatemeh Fallah Mohammad Ali Malekan Haleh Talaie |
| author_facet | Sepideh Kamalbeik Mehran Kouchek Majid Baseri Salehi Fatemeh Fallah Mohammad Ali Malekan Haleh Talaie |
| author_sort | Sepideh Kamalbeik |
| collection | DOAJ |
| description | Background: Acinetobacter baumannii is an important opportunistic pathogen which causes complications in hospitalized patients, especially those in ICU. The aim of this study was to determine the frequency of class 1 and 2 integrons in multi-drug resistance A. baumannii and to investigate the association between the presence of integrons and antibiotic resistance patterns.
Methods: A total of 40 A. baumannii strains were isolated from 372 ICU patients from June to Oct 2012. A. baumannii was detected in 50% of tracheal cultures, 15% in blood, 15% in urine samples, and 22.5% in other locations. In accordance with CLSI 2011, 12 antibiotics were used through disc diffusion method. Existence of integron classes was investigated by PCR assay with the amplification of integrase genes.
Results: The most effective antibiotic against Acinetobacter baumannii was polymyxin B with 100% susceptibility, followed by meropenem, piperacillin, cotrimoxazole, ceftazidime with 100% resistance; this was followed by ciprofloxacin 97.5%, tetracycline, 92.5%, imipenem 62.5%, and gentamicin 60% resistance. The presence of integron class 1 was 7.5%, class 2 was 67.5%, and non-integron was 20%.
Conclusion: The association between multidrug resistance and class 2 integron was not statistically significant. Other factors accounting for the lack of significance of the findings may be the impact of other resistance determinants such as transposons or plasmids, not investigated in the current study. Considering the increasing trend of MDR infections among ICU patients with critical problems in follow up, the use of appropriate infection control strategy and a regular surveillance system is necessary in our hospital. |
| first_indexed | 2024-04-13T06:52:32Z |
| format | Article |
| id | doaj.art-b8be048699234213ad2ac5d6fa17316c |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-04-13T06:52:32Z |
| publishDate | 2016-07-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-b8be048699234213ad2ac5d6fa17316c2022-12-22T02:57:21ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592016-07-01722900906Prevalence of Class 2 Integrons in Multidrug-Resistant Acinetobacter Baumannii in Toxicological ICU Patients in TehranSepideh Kamalbeik0Mehran Kouchek1Majid Baseri Salehi2Fatemeh Fallah3Mohammad Ali Malekan4Haleh Talaie5Department of Microbiology, Islamic Azad University Fars Science and Research Branch, Shiraz, Iran.Department of ICU of Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Microbiology, Islamic Azad University Fars Science and Research Branch, Shiraz, Iran.PhD of Bacteriology, Pediatric Infections Research Center, Mofid Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.PhD of Bacteriology, Pediatric Infections Research Center, Mofid Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Proffesor of Toxicological Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. *Corresponding Author: E-mail: talaie@sbmu.ac.irBackground: Acinetobacter baumannii is an important opportunistic pathogen which causes complications in hospitalized patients, especially those in ICU. The aim of this study was to determine the frequency of class 1 and 2 integrons in multi-drug resistance A. baumannii and to investigate the association between the presence of integrons and antibiotic resistance patterns. Methods: A total of 40 A. baumannii strains were isolated from 372 ICU patients from June to Oct 2012. A. baumannii was detected in 50% of tracheal cultures, 15% in blood, 15% in urine samples, and 22.5% in other locations. In accordance with CLSI 2011, 12 antibiotics were used through disc diffusion method. Existence of integron classes was investigated by PCR assay with the amplification of integrase genes. Results: The most effective antibiotic against Acinetobacter baumannii was polymyxin B with 100% susceptibility, followed by meropenem, piperacillin, cotrimoxazole, ceftazidime with 100% resistance; this was followed by ciprofloxacin 97.5%, tetracycline, 92.5%, imipenem 62.5%, and gentamicin 60% resistance. The presence of integron class 1 was 7.5%, class 2 was 67.5%, and non-integron was 20%. Conclusion: The association between multidrug resistance and class 2 integron was not statistically significant. Other factors accounting for the lack of significance of the findings may be the impact of other resistance determinants such as transposons or plasmids, not investigated in the current study. Considering the increasing trend of MDR infections among ICU patients with critical problems in follow up, the use of appropriate infection control strategy and a regular surveillance system is necessary in our hospital.http://ijt.arakmu.ac.ir/browse.php?a_id=258&sid=1&slc_lang=enAcinetobacter BaumanniiClass 1 IntegronClass 2 IntegronMultidrug ResistancePCR Assay. |
| spellingShingle | Sepideh Kamalbeik Mehran Kouchek Majid Baseri Salehi Fatemeh Fallah Mohammad Ali Malekan Haleh Talaie Prevalence of Class 2 Integrons in Multidrug-Resistant Acinetobacter Baumannii in Toxicological ICU Patients in Tehran Iranian Journal of Toxicology Acinetobacter Baumannii Class 1 Integron Class 2 Integron Multidrug Resistance PCR Assay. |
| title | Prevalence of Class 2 Integrons in Multidrug-Resistant Acinetobacter Baumannii in Toxicological ICU Patients in Tehran |
| title_full | Prevalence of Class 2 Integrons in Multidrug-Resistant Acinetobacter Baumannii in Toxicological ICU Patients in Tehran |
| title_fullStr | Prevalence of Class 2 Integrons in Multidrug-Resistant Acinetobacter Baumannii in Toxicological ICU Patients in Tehran |
| title_full_unstemmed | Prevalence of Class 2 Integrons in Multidrug-Resistant Acinetobacter Baumannii in Toxicological ICU Patients in Tehran |
| title_short | Prevalence of Class 2 Integrons in Multidrug-Resistant Acinetobacter Baumannii in Toxicological ICU Patients in Tehran |
| title_sort | prevalence of class 2 integrons in multidrug resistant acinetobacter baumannii in toxicological icu patients in tehran |
| topic | Acinetobacter Baumannii Class 1 Integron Class 2 Integron Multidrug Resistance PCR Assay. |
| url | http://ijt.arakmu.ac.ir/browse.php?a_id=258&sid=1&slc_lang=en |
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