Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System
Genome editing offers great advantages in identifying gene function and generating agronomically important mutations in crops. Here, we report that the genome of cabbage (Brassica oleracea var. capitata), an important cruciferous vegetable, can also be precisely edited by a CRISPR/Cas9 system stacke...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2019-07-01
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| Series: | Horticultural Plant Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014119300950 |
| _version_ | 1797203186228920320 |
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| author | Cunfa Ma Mengci Liu Qinfei Li Jun Si Xuesong Ren Hongyuan Song |
| author_facet | Cunfa Ma Mengci Liu Qinfei Li Jun Si Xuesong Ren Hongyuan Song |
| author_sort | Cunfa Ma |
| collection | DOAJ |
| description | Genome editing offers great advantages in identifying gene function and generating agronomically important mutations in crops. Here, we report that the genome of cabbage (Brassica oleracea var. capitata), an important cruciferous vegetable, can also be precisely edited by a CRISPR/Cas9 system stacked with multiple single-guide RNA-expressing cassettes. When the phytoene desaturase BoPDS gene was used as the target gene, an albino-phenotype transgenic shoot in T0 Basta-resistant lines was observed, and 37.5% of the transgenic cabbage shoots carried BoPDS gene mutations as a result of nucleotide deletions at the expected position. Moreover, mutations were detected in sites with the same target sequence in gene Bol016089 which is paralogous to the BoPDS gene. Our results show that the CRISPR/ Cas9 system is a powerful tool for cabbage variety improvement by genome editing. Keywords: Cabbage, CRISPR/Cas9, BoPDS, Genome editing, Mutation |
| first_indexed | 2024-04-24T08:15:20Z |
| format | Article |
| id | doaj.art-b8be1f7e68d640ecad73e4e6d3279799 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:15:20Z |
| publishDate | 2019-07-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-b8be1f7e68d640ecad73e4e6d32797992024-04-17T04:18:56ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412019-07-0154164169Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 SystemCunfa Ma0Mengci Liu1Qinfei Li2Jun Si3Xuesong Ren4Hongyuan Song5Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaKey Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCorresponding authors. Tel.: +86 23 68251158; Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCorresponding authors. Tel.: +86 23 68251158; Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaGenome editing offers great advantages in identifying gene function and generating agronomically important mutations in crops. Here, we report that the genome of cabbage (Brassica oleracea var. capitata), an important cruciferous vegetable, can also be precisely edited by a CRISPR/Cas9 system stacked with multiple single-guide RNA-expressing cassettes. When the phytoene desaturase BoPDS gene was used as the target gene, an albino-phenotype transgenic shoot in T0 Basta-resistant lines was observed, and 37.5% of the transgenic cabbage shoots carried BoPDS gene mutations as a result of nucleotide deletions at the expected position. Moreover, mutations were detected in sites with the same target sequence in gene Bol016089 which is paralogous to the BoPDS gene. Our results show that the CRISPR/ Cas9 system is a powerful tool for cabbage variety improvement by genome editing. Keywords: Cabbage, CRISPR/Cas9, BoPDS, Genome editing, Mutationhttp://www.sciencedirect.com/science/article/pii/S2468014119300950 |
| spellingShingle | Cunfa Ma Mengci Liu Qinfei Li Jun Si Xuesong Ren Hongyuan Song Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System Horticultural Plant Journal |
| title | Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System |
| title_full | Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System |
| title_fullStr | Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System |
| title_full_unstemmed | Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System |
| title_short | Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System |
| title_sort | efficient bopds gene editing in cabbage by the crispr cas9 system |
| url | http://www.sciencedirect.com/science/article/pii/S2468014119300950 |
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