Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer
Aim: Data about the relationship between oxidative stress and cancer pathogenesis has been increased in recent years. Thiol and disulphide play an important role in cell signal mechanisms, antioxidant protection, and detoxification. In this study, we aimed to evaluate the role of Thiol-Disulphide ho...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Turkiye Klinikleri
2019-08-01
|
| Series: | Journal of Oncological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452336419300329 |
| _version_ | 1797915053602635776 |
|---|---|
| author | Burak Bilgin Mehmet AN. Sendur Mutlu Hizal Sümeyye Ulutas Kandil Samet Yaman Muhammed Bülent Akıncı Didem Şener Dede Salim Neselioglu Özcan Erel Bülent Yalçın |
| author_facet | Burak Bilgin Mehmet AN. Sendur Mutlu Hizal Sümeyye Ulutas Kandil Samet Yaman Muhammed Bülent Akıncı Didem Şener Dede Salim Neselioglu Özcan Erel Bülent Yalçın |
| author_sort | Burak Bilgin |
| collection | DOAJ |
| description | Aim: Data about the relationship between oxidative stress and cancer pathogenesis has been increased in recent years. Thiol and disulphide play an important role in cell signal mechanisms, antioxidant protection, and detoxification. In this study, we aimed to evaluate the role of Thiol-Disulphide homeostasis (TDH) in colorectal cancer (CRC) by using a new method. Material and Method: The patients (pts) who diagnosed with CRC and healthy control subjects were included to study. Serum samples for the thiol-disulphide test were obtained at the time of diagnosis. TDH tests were measured by the automated spectrophotometric method by describing Erel and Neselioğlu. Thiol-disulphide homeostasis was also evaluated according to tumor stage and localization. Results: Eighty-eight pts with CRC and 110 control were enrolled. Native thiol (NT), disulphide and total thiol (TT) levels were significantly lower in patients compared with the control arm (Median NT: 402–424, p = 0.003; median Disulphide 18.7–21, p = 0.011; median TT: 437–467, p = 0.001). Thiol/disulphide balance was also maintained (p = 0.149). TT and NT levels were not differed according to tumor localization whereas disulphide level was significantly higher in left-sided tumors than right-sided (19.9–13.07 respectively, p = 0.007). In addition, disulphide/NT ratio was also significantly higher in left-sided than right-sided (0.1–0.12 respectively, p = 0.015). Thus, the balance of dynamic TDH is disrupted in favor of disulphide between left-sided and right-sided tumor. There was also no significant difference between thiol-disulphide levels and tumor stage whereas thiol level tends to lower in stage 4 disease (p = 0.7). Conclusion: This is the first trial that evaluates the relationship with dynamic TDH and CRC according to tumor stage and localization. Thiol and disulphide may play an important role in the pathogenesis of CRC. Keywords: Colorectal cancer, Disulphide, Oxidative stress, Thiols |
| first_indexed | 2024-04-10T12:36:39Z |
| format | Article |
| id | doaj.art-b8bf8949e77d4e11827432caa59a2406 |
| institution | Directory Open Access Journal |
| issn | 2452-3364 |
| language | English |
| last_indexed | 2024-04-10T12:36:39Z |
| publishDate | 2019-08-01 |
| publisher | Turkiye Klinikleri |
| record_format | Article |
| series | Journal of Oncological Sciences |
| spelling | doaj.art-b8bf8949e77d4e11827432caa59a24062023-02-15T16:14:38ZengTurkiye KlinikleriJournal of Oncological Sciences2452-33642019-08-01526064Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancerBurak Bilgin0Mehmet AN. Sendur1Mutlu Hizal2Sümeyye Ulutas Kandil3Samet Yaman4Muhammed Bülent Akıncı5Didem Şener Dede6Salim Neselioglu7Özcan Erel8Bülent Yalçın9Ankara Yıldırım Beyazıt University, Faculty of Medicine, Department of Medical Oncology, Ankara, Turkey; Corresponding author. Ankara Ataturk Chest Disease and Chest Surgery Research and Training Hospital, Department of Medical Oncology, 06100, Kecioren, Ankara, Turkey.Ankara Yıldırım Beyazıt University, Faculty of Medicine, Department of Medical Oncology, Ankara, TurkeyAnkara Yıldırım Beyazıt University, Faculty of Medicine, Department of Medical Oncology, Ankara, TurkeyAnkara Yıldırım Beyazıt University, Faculty of Medicine, Department of Internal Medicine, Ankara, TurkeyAnkara Yıldırım Beyazıt University, Faculty of Medicine, Department of Internal Medicine, Ankara, TurkeyAnkara Yıldırım Beyazıt University, Faculty of Medicine, Department of Medical Oncology, Ankara, TurkeyAnkara Yıldırım Beyazıt University, Faculty of Medicine, Department of Medical Oncology, Ankara, Turkey; Ankara Yıldırım Beyazıt University, Faculty of Medicine, Department of Medical Oncology, Ankara, TurkeyAnkara Yıldırım Beyazıt University, Faculty of Medicine, Department of Biochemistry, Ankara, TurkeyAnkara Yıldırım Beyazıt University, Faculty of Medicine, Department of Biochemistry, Ankara, TurkeyAnkara Yıldırım Beyazıt University, Faculty of Medicine, Department of Medical Oncology, Ankara, TurkeyAim: Data about the relationship between oxidative stress and cancer pathogenesis has been increased in recent years. Thiol and disulphide play an important role in cell signal mechanisms, antioxidant protection, and detoxification. In this study, we aimed to evaluate the role of Thiol-Disulphide homeostasis (TDH) in colorectal cancer (CRC) by using a new method. Material and Method: The patients (pts) who diagnosed with CRC and healthy control subjects were included to study. Serum samples for the thiol-disulphide test were obtained at the time of diagnosis. TDH tests were measured by the automated spectrophotometric method by describing Erel and Neselioğlu. Thiol-disulphide homeostasis was also evaluated according to tumor stage and localization. Results: Eighty-eight pts with CRC and 110 control were enrolled. Native thiol (NT), disulphide and total thiol (TT) levels were significantly lower in patients compared with the control arm (Median NT: 402–424, p = 0.003; median Disulphide 18.7–21, p = 0.011; median TT: 437–467, p = 0.001). Thiol/disulphide balance was also maintained (p = 0.149). TT and NT levels were not differed according to tumor localization whereas disulphide level was significantly higher in left-sided tumors than right-sided (19.9–13.07 respectively, p = 0.007). In addition, disulphide/NT ratio was also significantly higher in left-sided than right-sided (0.1–0.12 respectively, p = 0.015). Thus, the balance of dynamic TDH is disrupted in favor of disulphide between left-sided and right-sided tumor. There was also no significant difference between thiol-disulphide levels and tumor stage whereas thiol level tends to lower in stage 4 disease (p = 0.7). Conclusion: This is the first trial that evaluates the relationship with dynamic TDH and CRC according to tumor stage and localization. Thiol and disulphide may play an important role in the pathogenesis of CRC. Keywords: Colorectal cancer, Disulphide, Oxidative stress, Thiolshttp://www.sciencedirect.com/science/article/pii/S2452336419300329 |
| spellingShingle | Burak Bilgin Mehmet AN. Sendur Mutlu Hizal Sümeyye Ulutas Kandil Samet Yaman Muhammed Bülent Akıncı Didem Şener Dede Salim Neselioglu Özcan Erel Bülent Yalçın Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer Journal of Oncological Sciences |
| title | Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer |
| title_full | Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer |
| title_fullStr | Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer |
| title_full_unstemmed | Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer |
| title_short | Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer |
| title_sort | evaluation of dynamic serum thiol disulphide homeostasis in colorectal cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2452336419300329 |
| work_keys_str_mv | AT burakbilgin evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer AT mehmetansendur evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer AT mutluhizal evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer AT sumeyyeulutaskandil evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer AT sametyaman evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer AT muhammedbulentakıncı evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer AT didemsenerdede evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer AT salimneselioglu evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer AT ozcanerel evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer AT bulentyalcın evaluationofdynamicserumthioldisulphidehomeostasisincolorectalcancer |