A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging
The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocy...
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MDPI AG
2020-08-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/17/4768 |
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| author | Zongcheng Wang Huihuang Zheng Chengliang Zhang Dongfang Tang Qiyao Wu Wubliker Dessie Yuren Jiang |
| author_facet | Zongcheng Wang Huihuang Zheng Chengliang Zhang Dongfang Tang Qiyao Wu Wubliker Dessie Yuren Jiang |
| author_sort | Zongcheng Wang |
| collection | DOAJ |
| description | The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound <b>YZ-A4</b> as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor <b>YZ-A4</b> to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that <b>YZ-A4</b> could be used for fluorescence imaging of selenocysteine in living cells. |
| first_indexed | 2024-03-10T16:56:08Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T16:56:08Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-b8c4fcc916aa42c89f96bbabdc5809aa2023-11-20T11:08:27ZengMDPI AGSensors1424-82202020-08-012017476810.3390/s20174768A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells ImagingZongcheng Wang0Huihuang Zheng1Chengliang Zhang2Dongfang Tang3Qiyao Wu4Wubliker Dessie5Yuren Jiang6College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaCollege of Chemical and Biological Engineering, Hunan University of Science and Engineering, Yongzhou 425199, ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaCollege of Chemical and Biological Engineering, Hunan University of Science and Engineering, Yongzhou 425199, ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaCollege of Chemical and Biological Engineering, Hunan University of Science and Engineering, Yongzhou 425199, ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaThe content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound <b>YZ-A4</b> as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor <b>YZ-A4</b> to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that <b>YZ-A4</b> could be used for fluorescence imaging of selenocysteine in living cells.https://www.mdpi.com/1424-8220/20/17/4768fluorescent sensorselenocysteinelong-wavelengthliving cell fluorescence imaging |
| spellingShingle | Zongcheng Wang Huihuang Zheng Chengliang Zhang Dongfang Tang Qiyao Wu Wubliker Dessie Yuren Jiang A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging Sensors fluorescent sensor selenocysteine long-wavelength living cell fluorescence imaging |
| title | A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging |
| title_full | A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging |
| title_fullStr | A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging |
| title_full_unstemmed | A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging |
| title_short | A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging |
| title_sort | red emissive fluorescent turn on sensor for the rapid detection of selenocysteine and its application in living cells imaging |
| topic | fluorescent sensor selenocysteine long-wavelength living cell fluorescence imaging |
| url | https://www.mdpi.com/1424-8220/20/17/4768 |
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