Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease
The present study aimed to determine the effects of polysaccharides-riched Prunus mume fruit juice concentrate (PFC) on uric acid (UA) excretion and the gut microbiota in mice with chronic kidney disease (CKD). C57BL/6 mice were randomly allocated to four groups: two that were fed AIN93M diet, one o...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927122001988 |
| _version_ | 1811188530631671808 |
|---|---|
| author | Yan Huang Chen-Xi Wu Lu Guo Xiao-Xi Zhang Dao-Zong Xia |
| author_facet | Yan Huang Chen-Xi Wu Lu Guo Xiao-Xi Zhang Dao-Zong Xia |
| author_sort | Yan Huang |
| collection | DOAJ |
| description | The present study aimed to determine the effects of polysaccharides-riched Prunus mume fruit juice concentrate (PFC) on uric acid (UA) excretion and the gut microbiota in mice with chronic kidney disease (CKD). C57BL/6 mice were randomly allocated to four groups: two that were fed AIN93M diet, one of which was administered 500 mg/kg PFC, and two that were fed AIN93M diet containing 0.2% adenine, one of which was administered 500 mg/kg PFC. PFC promoted UA excretion, which may have been mediated through increases in the protein expression of ATP-binding cassette transporter G2 (ABCG2), organic anion transporter 1 (OAT1), organic carnitine transporter 2 (OCTN2), and reductions in the protein expression of glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1) in kidneys of CKD mice. ABCG2 expression in the intestine was also increased by PFC administration. Additionally, PFC significantly increased large intestinal short-chain fatty acids (SCFAs) concentrations, and the number of gut microbial species, and reduced the abundance of the genera Bacteroides, Pseudoflavonifractor, Helicobacter, Clostridium_IV and Allobaculum, which have a negative effect on UA excretion. In conclusion, PFC may promote UA excretion in CKD mice by altering the expression of urate transporters and regulating the gut microbiota. |
| first_indexed | 2024-04-11T14:20:16Z |
| format | Article |
| id | doaj.art-b8c6ca47cdcd4104b39008cff670b1d1 |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-04-11T14:20:16Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-b8c6ca47cdcd4104b39008cff670b1d12022-12-22T04:19:04ZengElsevierCurrent Research in Food Science2665-92712022-01-01521352145Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney diseaseYan Huang0Chen-Xi Wu1Lu Guo2Xiao-Xi Zhang3Dao-Zong Xia4School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, ChinaCorresponding author.; School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, ChinaThe present study aimed to determine the effects of polysaccharides-riched Prunus mume fruit juice concentrate (PFC) on uric acid (UA) excretion and the gut microbiota in mice with chronic kidney disease (CKD). C57BL/6 mice were randomly allocated to four groups: two that were fed AIN93M diet, one of which was administered 500 mg/kg PFC, and two that were fed AIN93M diet containing 0.2% adenine, one of which was administered 500 mg/kg PFC. PFC promoted UA excretion, which may have been mediated through increases in the protein expression of ATP-binding cassette transporter G2 (ABCG2), organic anion transporter 1 (OAT1), organic carnitine transporter 2 (OCTN2), and reductions in the protein expression of glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1) in kidneys of CKD mice. ABCG2 expression in the intestine was also increased by PFC administration. Additionally, PFC significantly increased large intestinal short-chain fatty acids (SCFAs) concentrations, and the number of gut microbial species, and reduced the abundance of the genera Bacteroides, Pseudoflavonifractor, Helicobacter, Clostridium_IV and Allobaculum, which have a negative effect on UA excretion. In conclusion, PFC may promote UA excretion in CKD mice by altering the expression of urate transporters and regulating the gut microbiota.http://www.sciencedirect.com/science/article/pii/S2665927122001988Prunus mumeUrate transportersGut microbiotaShort-chain fatty acidsChronic kidney disease |
| spellingShingle | Yan Huang Chen-Xi Wu Lu Guo Xiao-Xi Zhang Dao-Zong Xia Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease Current Research in Food Science Prunus mume Urate transporters Gut microbiota Short-chain fatty acids Chronic kidney disease |
| title | Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease |
| title_full | Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease |
| title_fullStr | Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease |
| title_full_unstemmed | Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease |
| title_short | Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease |
| title_sort | effects of polysaccharides riched prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine induced chronic kidney disease |
| topic | Prunus mume Urate transporters Gut microbiota Short-chain fatty acids Chronic kidney disease |
| url | http://www.sciencedirect.com/science/article/pii/S2665927122001988 |
| work_keys_str_mv | AT yanhuang effectsofpolysaccharidesrichedprunusmumefruitjuiceconcentrateonuricacidexcretionandgutmicrobiotainmicewithadenineinducedchronickidneydisease AT chenxiwu effectsofpolysaccharidesrichedprunusmumefruitjuiceconcentrateonuricacidexcretionandgutmicrobiotainmicewithadenineinducedchronickidneydisease AT luguo effectsofpolysaccharidesrichedprunusmumefruitjuiceconcentrateonuricacidexcretionandgutmicrobiotainmicewithadenineinducedchronickidneydisease AT xiaoxizhang effectsofpolysaccharidesrichedprunusmumefruitjuiceconcentrateonuricacidexcretionandgutmicrobiotainmicewithadenineinducedchronickidneydisease AT daozongxia effectsofpolysaccharidesrichedprunusmumefruitjuiceconcentrateonuricacidexcretionandgutmicrobiotainmicewithadenineinducedchronickidneydisease |