Cell Therapy with Encapsulated Xenogeneic Tumor Cells Secreting β-Endorphin for Treatment of Peripheral Pain
The purpose of this study was to assess whether xenogeneic tumor cells secreting β-endorphin and immunologically isolated in polymer capsules could survive and continue to reduce pain when transplanted into the spinal cerebro-spinal fluid (CSF) space of rats. Also, a silicone container for polymer c...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
1995-01-01
|
| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368979500401S05 |
| _version_ | 1818342344101986304 |
|---|---|
| author | Youichi Saitoh Takuyu Taki Norio Arita Takanori Ohnishi Toru Hayakawa |
| author_facet | Youichi Saitoh Takuyu Taki Norio Arita Takanori Ohnishi Toru Hayakawa |
| author_sort | Youichi Saitoh |
| collection | DOAJ |
| description | The purpose of this study was to assess whether xenogeneic tumor cells secreting β-endorphin and immunologically isolated in polymer capsules could survive and continue to reduce pain when transplanted into the spinal cerebro-spinal fluid (CSF) space of rats. Also, a silicone container for polymer capsules was designed for the clinical application of this method of cell therapy. The mouse tumor cell lines, proopiomelanocortin gene transfected Neuro2A which secrete β-endorphin, were enclosed in polymer capsules at a density of 5 x 10 6 /mL, and transplanted into the spinal CSF space from the occipito-atlantal junction of male Sprague-Dawley rats. Three analgesiometric tests —the tail pinch test, the hot plate test, and electrical stimulation test — showed that the rats with encapsulated Neuro2A (n = 6) were significantly less sensitive to pain after transplantation than control animals (n = 8). The analgesia induced by the encapsulated cells secreting β-endorphin was attenuated by the opiate antagonist naloxone. Morphological study revealed that the encapsulated cells survived for 1 mo after transplantation into the CSF space. An in vitro experiment on cultured capsules (3 cm long) with a silicone container (Kaneka Medics Co) showed that the encapsulated Neuro2A (5 x 10 6 mL) could secrete peptides for 1 mo. The results of this study indicate that immunologically isolated xenogeneic tumor cells can secrete opiate in the CSF space, and that a silicone container may help the application of this method to the treatment of cancer pain. |
| first_indexed | 2024-12-13T16:13:11Z |
| format | Article |
| id | doaj.art-b8c6e14b45d44f47925cee88e043ba5f |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-13T16:13:11Z |
| publishDate | 1995-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-b8c6e14b45d44f47925cee88e043ba5f2022-12-21T23:38:53ZengSAGE PublishingCell Transplantation0963-68971555-38921995-01-01410.1177/096368979500401S05Cell Therapy with Encapsulated Xenogeneic Tumor Cells Secreting β-Endorphin for Treatment of Peripheral PainYouichi Saitoh0Takuyu Taki1Norio Arita2Takanori Ohnishi3Toru Hayakawa4Department of Neurosurgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565, JapanDepartment of Neurosurgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565, JapanDepartment of Neurosurgery, Kinki University Medical School, 377-2 Ohnohigashi, Sayama, Osaka 589, JapanDepartment of Neurosurgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565, JapanDepartment of Neurosurgery, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565, JapanThe purpose of this study was to assess whether xenogeneic tumor cells secreting β-endorphin and immunologically isolated in polymer capsules could survive and continue to reduce pain when transplanted into the spinal cerebro-spinal fluid (CSF) space of rats. Also, a silicone container for polymer capsules was designed for the clinical application of this method of cell therapy. The mouse tumor cell lines, proopiomelanocortin gene transfected Neuro2A which secrete β-endorphin, were enclosed in polymer capsules at a density of 5 x 10 6 /mL, and transplanted into the spinal CSF space from the occipito-atlantal junction of male Sprague-Dawley rats. Three analgesiometric tests —the tail pinch test, the hot plate test, and electrical stimulation test — showed that the rats with encapsulated Neuro2A (n = 6) were significantly less sensitive to pain after transplantation than control animals (n = 8). The analgesia induced by the encapsulated cells secreting β-endorphin was attenuated by the opiate antagonist naloxone. Morphological study revealed that the encapsulated cells survived for 1 mo after transplantation into the CSF space. An in vitro experiment on cultured capsules (3 cm long) with a silicone container (Kaneka Medics Co) showed that the encapsulated Neuro2A (5 x 10 6 mL) could secrete peptides for 1 mo. The results of this study indicate that immunologically isolated xenogeneic tumor cells can secrete opiate in the CSF space, and that a silicone container may help the application of this method to the treatment of cancer pain.https://doi.org/10.1177/096368979500401S05 |
| spellingShingle | Youichi Saitoh Takuyu Taki Norio Arita Takanori Ohnishi Toru Hayakawa Cell Therapy with Encapsulated Xenogeneic Tumor Cells Secreting β-Endorphin for Treatment of Peripheral Pain Cell Transplantation |
| title | Cell Therapy with Encapsulated Xenogeneic Tumor Cells Secreting β-Endorphin for Treatment of Peripheral Pain |
| title_full | Cell Therapy with Encapsulated Xenogeneic Tumor Cells Secreting β-Endorphin for Treatment of Peripheral Pain |
| title_fullStr | Cell Therapy with Encapsulated Xenogeneic Tumor Cells Secreting β-Endorphin for Treatment of Peripheral Pain |
| title_full_unstemmed | Cell Therapy with Encapsulated Xenogeneic Tumor Cells Secreting β-Endorphin for Treatment of Peripheral Pain |
| title_short | Cell Therapy with Encapsulated Xenogeneic Tumor Cells Secreting β-Endorphin for Treatment of Peripheral Pain |
| title_sort | cell therapy with encapsulated xenogeneic tumor cells secreting β endorphin for treatment of peripheral pain |
| url | https://doi.org/10.1177/096368979500401S05 |
| work_keys_str_mv | AT youichisaitoh celltherapywithencapsulatedxenogeneictumorcellssecretingbendorphinfortreatmentofperipheralpain AT takuyutaki celltherapywithencapsulatedxenogeneictumorcellssecretingbendorphinfortreatmentofperipheralpain AT norioarita celltherapywithencapsulatedxenogeneictumorcellssecretingbendorphinfortreatmentofperipheralpain AT takanoriohnishi celltherapywithencapsulatedxenogeneictumorcellssecretingbendorphinfortreatmentofperipheralpain AT toruhayakawa celltherapywithencapsulatedxenogeneictumorcellssecretingbendorphinfortreatmentofperipheralpain |