Transwell migration assay to interrogate human CAR-T cell chemotaxis
Summary: A major impediment to effective cellular therapies in solid tumors is the limited access of therapeutic cells to the tumor site. One strategy to overcome this challenge is to endow T cells with chemotactic properties required to access tumor tissue. Here, we present a chimeric antigen recep...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722005883 |
| _version_ | 1811292558599389184 |
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| author | Arman Oner Sebastian Kobold |
| author_facet | Arman Oner Sebastian Kobold |
| author_sort | Arman Oner |
| collection | DOAJ |
| description | Summary: A major impediment to effective cellular therapies in solid tumors is the limited access of therapeutic cells to the tumor site. One strategy to overcome this challenge is to endow T cells with chemotactic properties required to access tumor tissue. Here, we present a chimeric antigen receptor (CAR)-modified T cell strategy centered around enhanced T cell trafficking. We outline isolation, activation, and transduction of human T cells, as well as techniques for assessing migratory and cytotoxic capacity of CAR-T cells.For complete details on the use and execution of this protocol, please refer to Lesch et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T04:47:14Z |
| format | Article |
| id | doaj.art-b8c79f264c094e8d9762ca37a3d753bd |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T04:47:14Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-b8c79f264c094e8d9762ca37a3d753bd2022-12-22T03:01:49ZengElsevierSTAR Protocols2666-16672022-12-0134101708Transwell migration assay to interrogate human CAR-T cell chemotaxisArman Oner0Sebastian Kobold1Division of Clinical Pharmacology, Department of Medicine IV, Klinikum der Universität München, LMU Munich, Munich, Germany; Corresponding authorDivision of Clinical Pharmacology, Department of Medicine IV, Klinikum der Universität München, LMU Munich, Munich, Germany; Einheit für Klinische Pharmakologie (EKLiP), Helmholtz Zentrum München, Research Center for Environmental Health (HMGU), Neuherberg, Germany; German Center for Translational Cancer Research (DKTK), Partner Site Munich, Munich, Germany; Corresponding authorSummary: A major impediment to effective cellular therapies in solid tumors is the limited access of therapeutic cells to the tumor site. One strategy to overcome this challenge is to endow T cells with chemotactic properties required to access tumor tissue. Here, we present a chimeric antigen receptor (CAR)-modified T cell strategy centered around enhanced T cell trafficking. We outline isolation, activation, and transduction of human T cells, as well as techniques for assessing migratory and cytotoxic capacity of CAR-T cells.For complete details on the use and execution of this protocol, please refer to Lesch et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722005883Cell isolationCell-based assaysCancerImmunologyBiotechnology and bioengineering |
| spellingShingle | Arman Oner Sebastian Kobold Transwell migration assay to interrogate human CAR-T cell chemotaxis STAR Protocols Cell isolation Cell-based assays Cancer Immunology Biotechnology and bioengineering |
| title | Transwell migration assay to interrogate human CAR-T cell chemotaxis |
| title_full | Transwell migration assay to interrogate human CAR-T cell chemotaxis |
| title_fullStr | Transwell migration assay to interrogate human CAR-T cell chemotaxis |
| title_full_unstemmed | Transwell migration assay to interrogate human CAR-T cell chemotaxis |
| title_short | Transwell migration assay to interrogate human CAR-T cell chemotaxis |
| title_sort | transwell migration assay to interrogate human car t cell chemotaxis |
| topic | Cell isolation Cell-based assays Cancer Immunology Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722005883 |
| work_keys_str_mv | AT armanoner transwellmigrationassaytointerrogatehumancartcellchemotaxis AT sebastiankobold transwellmigrationassaytointerrogatehumancartcellchemotaxis |