Volatile Metabolomic Composition of Vitex Species: Chemodiversity Insights and Acaricidal Activity
The Vitex genus (Lamiaceae) produces a plethora of metabolites that include ecdysteroids and terpenoids, some of which have demonstrated insect repellent properties. The volatile composition of several members of this genus has not been chemically defined, as many taxa are endemic to remote ecosyste...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-11-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01931/full |
| _version_ | 1818196466083037184 |
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| author | José G. de Sena Filho Ighor C. Barreto Avaldo O. Soares Filho Paulo C. L. Nogueira Adenir V. Teodoro Ana V. Cruz da Silva Haroudo S. Xavier Allívia R. C. Rabbani Daniel J. Spakowicz Jennifer M. Duringer |
| author_facet | José G. de Sena Filho Ighor C. Barreto Avaldo O. Soares Filho Paulo C. L. Nogueira Adenir V. Teodoro Ana V. Cruz da Silva Haroudo S. Xavier Allívia R. C. Rabbani Daniel J. Spakowicz Jennifer M. Duringer |
| author_sort | José G. de Sena Filho |
| collection | DOAJ |
| description | The Vitex genus (Lamiaceae) produces a plethora of metabolites that include ecdysteroids and terpenoids, some of which have demonstrated insect repellent properties. The volatile composition of several members of this genus has not been chemically defined, as many taxa are endemic to remote ecosystems. In this study, leaves were collected from the northeast of Brazil from Vitex capitata, V. megapotamica, V. gardneriana, and V. rufescens plants and examined for their chemical profile via GC-MS/FID of essential oil extracts. The analyses showed a diversity of terpenoids. Of particular note were seven-member ring sesquiterpenes which were present in great abundance; a dendrogram showed clades separating by the production of bicyclogermacrene, aromadendrane and 5,10-cycloaromadendrane sesquiterpenoids for the four species. Comparison of volatile metabolite profiles to 13 other Vitex species showed strong similarities in the production of some monoterpenes, but varied by their production of larger terpenes, especially those with gem-dimethylcyclopropyl subunits on seven-member ring compounds. From this work, we suggest that the sesquiterpene skeleton with seven member rings is a good chemosystematic biomarker candidate for the Vitex genus. Separation using this biomarker was then validated using Inter-Simple Sequence Repeat profiling. Lastly, experiments examining the toxicity of these four oils against the coconut mite Aceria guerreronis showed that only the oil of V. gardneriana had strong acaricidal activity, with an LC50 of 0.85 mg/mL, thus demonstrating its potential for use as a natural pesticide. |
| first_indexed | 2024-12-12T01:34:31Z |
| format | Article |
| id | doaj.art-b8c7b73c80c84082bde5f433ee841945 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T01:34:31Z |
| publishDate | 2017-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b8c7b73c80c84082bde5f433ee8419452022-12-22T00:42:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-11-01810.3389/fpls.2017.01931298260Volatile Metabolomic Composition of Vitex Species: Chemodiversity Insights and Acaricidal ActivityJosé G. de Sena Filho0Ighor C. Barreto1Avaldo O. Soares Filho2Paulo C. L. Nogueira3Adenir V. Teodoro4Ana V. Cruz da Silva5Haroudo S. Xavier6Allívia R. C. Rabbani7Daniel J. Spakowicz8Jennifer M. Duringer9Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA Tabuleiros Costeiros, Aracaju, BrazilEmpresa Brasileira de Pesquisa Agropecuária – EMBRAPA Tabuleiros Costeiros, Aracaju, BrazilHerbário HUESBVC, Departamento de Ciências Naturais, Universidade Estadual do Sudoeste da Bahia, Conquista, BrazilLaboratório de Pesquisa em Química Orgânica de Sergipe, Departamento de Química – CCET, Universidade Federal de Sergipe, São Cristóvão, BrazilEmpresa Brasileira de Pesquisa Agropecuária – EMBRAPA Tabuleiros Costeiros, Aracaju, BrazilEmpresa Brasileira de Pesquisa Agropecuária – EMBRAPA Tabuleiros Costeiros, Aracaju, BrazilDepartamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, BrazilBahia Federal Institute of Education, Science and Technology – IFBA, Porto Seguro, BrazilProgram in Comparative Biology and Bioinformatics, Yale University, New Haven, CT, United StatesDepartment of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United StatesThe Vitex genus (Lamiaceae) produces a plethora of metabolites that include ecdysteroids and terpenoids, some of which have demonstrated insect repellent properties. The volatile composition of several members of this genus has not been chemically defined, as many taxa are endemic to remote ecosystems. In this study, leaves were collected from the northeast of Brazil from Vitex capitata, V. megapotamica, V. gardneriana, and V. rufescens plants and examined for their chemical profile via GC-MS/FID of essential oil extracts. The analyses showed a diversity of terpenoids. Of particular note were seven-member ring sesquiterpenes which were present in great abundance; a dendrogram showed clades separating by the production of bicyclogermacrene, aromadendrane and 5,10-cycloaromadendrane sesquiterpenoids for the four species. Comparison of volatile metabolite profiles to 13 other Vitex species showed strong similarities in the production of some monoterpenes, but varied by their production of larger terpenes, especially those with gem-dimethylcyclopropyl subunits on seven-member ring compounds. From this work, we suggest that the sesquiterpene skeleton with seven member rings is a good chemosystematic biomarker candidate for the Vitex genus. Separation using this biomarker was then validated using Inter-Simple Sequence Repeat profiling. Lastly, experiments examining the toxicity of these four oils against the coconut mite Aceria guerreronis showed that only the oil of V. gardneriana had strong acaricidal activity, with an LC50 of 0.85 mg/mL, thus demonstrating its potential for use as a natural pesticide.http://journal.frontiersin.org/article/10.3389/fpls.2017.01931/fullVitex capitataVitex megapotamicaVitex gardnerianaVitex rufescenschemodiversity |
| spellingShingle | José G. de Sena Filho Ighor C. Barreto Avaldo O. Soares Filho Paulo C. L. Nogueira Adenir V. Teodoro Ana V. Cruz da Silva Haroudo S. Xavier Allívia R. C. Rabbani Daniel J. Spakowicz Jennifer M. Duringer Volatile Metabolomic Composition of Vitex Species: Chemodiversity Insights and Acaricidal Activity Frontiers in Plant Science Vitex capitata Vitex megapotamica Vitex gardneriana Vitex rufescens chemodiversity |
| title | Volatile Metabolomic Composition of Vitex Species: Chemodiversity Insights and Acaricidal Activity |
| title_full | Volatile Metabolomic Composition of Vitex Species: Chemodiversity Insights and Acaricidal Activity |
| title_fullStr | Volatile Metabolomic Composition of Vitex Species: Chemodiversity Insights and Acaricidal Activity |
| title_full_unstemmed | Volatile Metabolomic Composition of Vitex Species: Chemodiversity Insights and Acaricidal Activity |
| title_short | Volatile Metabolomic Composition of Vitex Species: Chemodiversity Insights and Acaricidal Activity |
| title_sort | volatile metabolomic composition of vitex species chemodiversity insights and acaricidal activity |
| topic | Vitex capitata Vitex megapotamica Vitex gardneriana Vitex rufescens chemodiversity |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01931/full |
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