Toll Like Receptor 4 (TLR4) and p65 Nuclear Factor Kappa B ( NF - ĸ B ) Expression in Monocyte Cell of Children with Steroid Resistant Nephrotic Syndrome
This research aimed to prove the difference of TLR4 and p65NF-kB subunit expression between children with steroid -sensitive nephrotic syndrome (SSNS) and those with steroid-resistent nephrotic syndrome (SRNS) and to find out the correlation between expression of TLR...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2014-05-01
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| Series: | Journal of Tropical Life Science |
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| Online Access: | http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/149/194 |
| _version_ | 1797641360039215104 |
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| author | Tikto Wahyono Krisni Subandiyah Loeki Enggar Fitri Agustina Tri Endharti |
| author_facet | Tikto Wahyono Krisni Subandiyah Loeki Enggar Fitri Agustina Tri Endharti |
| author_sort | Tikto Wahyono |
| collection | DOAJ |
| description | This research aimed to prove the difference of TLR4 and p65NF-kB subunit expression between children with steroid -sensitive nephrotic syndrome (SSNS) and those with steroid-resistent nephrotic syndrome (SRNS) and to find out the correlation between expression of TLR4 and p65NF-kB on children with SRNS. Twenty three patients of idiopathic nephrotic syndrome patients (INS) were enrolled in this study and classified into two groups those with SSNS (11 patients) and SRNS (12 patients). Peripheral Blood Mononuclear Cells (PBMC) isolation was performed, taken from 5 ml patients’ blood samples, followed by monocyte isolation of PMBC using monocyte culture. Examination of TLR4 and p65 NF-kB expression from monocyte's cells culture was performed using flow cytometry. The result showed that the differences of TLR4 expression between SSNS and SRNS was significant (p=0.012), The differences of p65 NF-kB expression between SSNS and SRNS showed an insignificant correlation (p=0.880). Pearson correlation test between the expression of TLR4 and p65 NF-kB showed no significant correlation (p=0.472), while Spearman correlation test to see the relationship between expression of TLR4 and SRNS produced correlation value of 0.512 and its importance p value was 0.013 (p<0.05). There is a significant difference of TLR4 expression between SRNS and SSNS and there is also a significant correlation between expression of TLR4 and SRNS, that proved TLR4 has an important role in steroid resistance of nephrotic syndrome. |
| first_indexed | 2024-03-11T13:44:30Z |
| format | Article |
| id | doaj.art-b8c8857363684cc985422512218da573 |
| institution | Directory Open Access Journal |
| issn | 2087-5517 |
| language | English |
| last_indexed | 2024-03-11T13:44:30Z |
| publishDate | 2014-05-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-b8c8857363684cc985422512218da5732023-11-02T10:52:40ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172014-05-01428288Toll Like Receptor 4 (TLR4) and p65 Nuclear Factor Kappa B ( NF - ĸ B ) Expression in Monocyte Cell of Children with Steroid Resistant Nephrotic SyndromeTikto Wahyono0Krisni Subandiyah1Loeki Enggar Fitri2 Agustina Tri Endharti3Ma gister Program in Biomedical Science, University of Brawijaya Department of Pediatric, dr. Saiful Anwar Hospital, Malang IndonesiaLaboratory of Parasitology, Faculty of Medicine University of Brawijaya, Malang IndonesiaLaboratory of Parasitology, Faculty of Medicine University of Brawijaya, Malang IndonesiaThis research aimed to prove the difference of TLR4 and p65NF-kB subunit expression between children with steroid -sensitive nephrotic syndrome (SSNS) and those with steroid-resistent nephrotic syndrome (SRNS) and to find out the correlation between expression of TLR4 and p65NF-kB on children with SRNS. Twenty three patients of idiopathic nephrotic syndrome patients (INS) were enrolled in this study and classified into two groups those with SSNS (11 patients) and SRNS (12 patients). Peripheral Blood Mononuclear Cells (PBMC) isolation was performed, taken from 5 ml patients’ blood samples, followed by monocyte isolation of PMBC using monocyte culture. Examination of TLR4 and p65 NF-kB expression from monocyte's cells culture was performed using flow cytometry. The result showed that the differences of TLR4 expression between SSNS and SRNS was significant (p=0.012), The differences of p65 NF-kB expression between SSNS and SRNS showed an insignificant correlation (p=0.880). Pearson correlation test between the expression of TLR4 and p65 NF-kB showed no significant correlation (p=0.472), while Spearman correlation test to see the relationship between expression of TLR4 and SRNS produced correlation value of 0.512 and its importance p value was 0.013 (p<0.05). There is a significant difference of TLR4 expression between SRNS and SSNS and there is also a significant correlation between expression of TLR4 and SRNS, that proved TLR4 has an important role in steroid resistance of nephrotic syndrome.http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/149/194INSSSNSSRNSTLR4 expressionp65 NF - kB expression |
| spellingShingle | Tikto Wahyono Krisni Subandiyah Loeki Enggar Fitri Agustina Tri Endharti Toll Like Receptor 4 (TLR4) and p65 Nuclear Factor Kappa B ( NF - ĸ B ) Expression in Monocyte Cell of Children with Steroid Resistant Nephrotic Syndrome Journal of Tropical Life Science INS SSNS SRNS TLR4 expression p65 NF - kB expression |
| title | Toll Like Receptor 4 (TLR4) and p65 Nuclear Factor Kappa B ( NF - ĸ B ) Expression in Monocyte Cell of Children with Steroid Resistant Nephrotic Syndrome |
| title_full | Toll Like Receptor 4 (TLR4) and p65 Nuclear Factor Kappa B ( NF - ĸ B ) Expression in Monocyte Cell of Children with Steroid Resistant Nephrotic Syndrome |
| title_fullStr | Toll Like Receptor 4 (TLR4) and p65 Nuclear Factor Kappa B ( NF - ĸ B ) Expression in Monocyte Cell of Children with Steroid Resistant Nephrotic Syndrome |
| title_full_unstemmed | Toll Like Receptor 4 (TLR4) and p65 Nuclear Factor Kappa B ( NF - ĸ B ) Expression in Monocyte Cell of Children with Steroid Resistant Nephrotic Syndrome |
| title_short | Toll Like Receptor 4 (TLR4) and p65 Nuclear Factor Kappa B ( NF - ĸ B ) Expression in Monocyte Cell of Children with Steroid Resistant Nephrotic Syndrome |
| title_sort | toll like receptor 4 tlr4 and p65 nuclear factor kappa b nf q b expression in monocyte cell of children with steroid resistant nephrotic syndrome |
| topic | INS SSNS SRNS TLR4 expression p65 NF - kB expression |
| url | http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/149/194 |
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