Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ × SJL/J inbred mice
The total body fat mass and serum concentration of total cholesterol, HDL cholesterol, and triglyceride (TG) differ between standard diet-fed female inbred mouse strains MRL/MpJ (MRL) and SJL/J (SJL) by 38–120% (P < 0.01). To investigate genetic regulation of obesity and serum lipid levels, we pe...
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| Format: | Article |
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Elsevier
2006-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520336622 |
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| author | Apurva K. Srivastava Subburaman Mohan Godfred L. Masinde Hongrun Yu David J. Baylink |
| author_facet | Apurva K. Srivastava Subburaman Mohan Godfred L. Masinde Hongrun Yu David J. Baylink |
| author_sort | Apurva K. Srivastava |
| collection | DOAJ |
| description | The total body fat mass and serum concentration of total cholesterol, HDL cholesterol, and triglyceride (TG) differ between standard diet-fed female inbred mouse strains MRL/MpJ (MRL) and SJL/J (SJL) by 38–120% (P < 0.01). To investigate genetic regulation of obesity and serum lipid levels, we performed a genome-wide linkage analysis in 621 MRL× SJL F2 female mice. Fat mass was affected by two significant loci, D11Mit36 [43.7 cM, logarithm of the odds ratio (LOD) 11.2] and D16Mit51 (50.3 cM, LOD 3.9), and one suggestive locus at D7Mit44 (50 cM, LOD 2.4). TG levels were affected by two novel loci at D1Mit43 (76 cM, LOD 3.8) and D12Mit201 (26 cM, LOD 4.1), and two suggestive loci on chromosomes 5 and 17. HDL and cholesterol concentrations were influenced by significant loci on chromosomes 1, 3, 5, 7, and 17 that were in the regions identified earlier for other strains of mice, except for a suggestive locus on chromosome 14 that was specific to the MRL × SJL cross. In summary, linkage analysis in MRL × SJL F2 mice disclosed novel loci affecting TG, HDL, and fat mass, a measure of obesity. Knowledge of the genes in these quantitative trait loci will enhance our understanding of obesity and lipid metabolism. |
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| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T07:25:01Z |
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| series | Journal of Lipid Research |
| spelling | doaj.art-b8c9474c3fde40cea5e6bc5f88d9ba202022-12-21T21:58:40ZengElsevierJournal of Lipid Research0022-22752006-01-01471123133Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ × SJL/J inbred miceApurva K. Srivastava0Subburaman Mohan1Godfred L. Masinde2Hongrun Yu3David J. Baylink4Musculoskeletal Disease Center, Loma Linda VA Health Care Systems, Loma Linda, CA 92357; Department of Medicine, Loma Linda University, Loma Linda, CA 92354Musculoskeletal Disease Center, Loma Linda VA Health Care Systems, Loma Linda, CA 92357; Department of Medicine, Loma Linda University, Loma Linda, CA 92354Musculoskeletal Disease Center, Loma Linda VA Health Care Systems, Loma Linda, CA 92357; Department of Medicine, Loma Linda University, Loma Linda, CA 92354Musculoskeletal Disease Center, Loma Linda VA Health Care Systems, Loma Linda, CA 92357; Department of Medicine, Loma Linda University, Loma Linda, CA 92354Musculoskeletal Disease Center, Loma Linda VA Health Care Systems, Loma Linda, CA 92357; Department of Medicine, Loma Linda University, Loma Linda, CA 92354The total body fat mass and serum concentration of total cholesterol, HDL cholesterol, and triglyceride (TG) differ between standard diet-fed female inbred mouse strains MRL/MpJ (MRL) and SJL/J (SJL) by 38–120% (P < 0.01). To investigate genetic regulation of obesity and serum lipid levels, we performed a genome-wide linkage analysis in 621 MRL× SJL F2 female mice. Fat mass was affected by two significant loci, D11Mit36 [43.7 cM, logarithm of the odds ratio (LOD) 11.2] and D16Mit51 (50.3 cM, LOD 3.9), and one suggestive locus at D7Mit44 (50 cM, LOD 2.4). TG levels were affected by two novel loci at D1Mit43 (76 cM, LOD 3.8) and D12Mit201 (26 cM, LOD 4.1), and two suggestive loci on chromosomes 5 and 17. HDL and cholesterol concentrations were influenced by significant loci on chromosomes 1, 3, 5, 7, and 17 that were in the regions identified earlier for other strains of mice, except for a suggestive locus on chromosome 14 that was specific to the MRL × SJL cross. In summary, linkage analysis in MRL × SJL F2 mice disclosed novel loci affecting TG, HDL, and fat mass, a measure of obesity. Knowledge of the genes in these quantitative trait loci will enhance our understanding of obesity and lipid metabolism.http://www.sciencedirect.com/science/article/pii/S0022227520336622body fat massHDL cholesterolcholesteroltriglyceride |
| spellingShingle | Apurva K. Srivastava Subburaman Mohan Godfred L. Masinde Hongrun Yu David J. Baylink Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ × SJL/J inbred mice Journal of Lipid Research body fat mass HDL cholesterol cholesterol triglyceride |
| title | Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ × SJL/J inbred mice |
| title_full | Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ × SJL/J inbred mice |
| title_fullStr | Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ × SJL/J inbred mice |
| title_full_unstemmed | Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ × SJL/J inbred mice |
| title_short | Identification of quantitative trait loci that regulate obesity and serum lipid levels in MRL/MpJ × SJL/J inbred mice |
| title_sort | identification of quantitative trait loci that regulate obesity and serum lipid levels in mrl mpj sjl j inbred mice |
| topic | body fat mass HDL cholesterol cholesterol triglyceride |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520336622 |
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