Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin.
eNOS activation resulting in mitochondrial biogenesis is believed to play a central role in life span extension promoted by calorie restriction (CR). We investigated the mechanism of this activation by treating vascular cells with serum from CR rats and found increased Akt and eNOS phosphorylation,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3271099?pdf=render |
| _version_ | 1811211925323776000 |
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| author | Fernanda M Cerqueira Laura I Brandizzi Fernanda M Cunha Francisco R M Laurindo Alicia J Kowaltowski |
| author_facet | Fernanda M Cerqueira Laura I Brandizzi Fernanda M Cunha Francisco R M Laurindo Alicia J Kowaltowski |
| author_sort | Fernanda M Cerqueira |
| collection | DOAJ |
| description | eNOS activation resulting in mitochondrial biogenesis is believed to play a central role in life span extension promoted by calorie restriction (CR). We investigated the mechanism of this activation by treating vascular cells with serum from CR rats and found increased Akt and eNOS phosphorylation, in addition to enhanced nitrite release. Inhibiting Akt phosphorylation or immunoprecipitating adiponectin (found in high quantities in CR serum) completely prevented the increment in nitrite release and eNOS activation. Overall, we demonstrate that adiponectin in the serum from CR animals increases NO• signaling by activating the insulin pathway. These results suggest this hormone may be a determinant regulator of the beneficial effects of CR. |
| first_indexed | 2024-04-12T05:21:39Z |
| format | Article |
| id | doaj.art-b8ca05b1786d4cc9953f4e72c2b6245d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T05:21:39Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b8ca05b1786d4cc9953f4e72c2b6245d2022-12-22T03:46:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3115510.1371/journal.pone.0031155Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin.Fernanda M CerqueiraLaura I BrandizziFernanda M CunhaFrancisco R M LaurindoAlicia J KowaltowskieNOS activation resulting in mitochondrial biogenesis is believed to play a central role in life span extension promoted by calorie restriction (CR). We investigated the mechanism of this activation by treating vascular cells with serum from CR rats and found increased Akt and eNOS phosphorylation, in addition to enhanced nitrite release. Inhibiting Akt phosphorylation or immunoprecipitating adiponectin (found in high quantities in CR serum) completely prevented the increment in nitrite release and eNOS activation. Overall, we demonstrate that adiponectin in the serum from CR animals increases NO• signaling by activating the insulin pathway. These results suggest this hormone may be a determinant regulator of the beneficial effects of CR.http://europepmc.org/articles/PMC3271099?pdf=render |
| spellingShingle | Fernanda M Cerqueira Laura I Brandizzi Fernanda M Cunha Francisco R M Laurindo Alicia J Kowaltowski Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin. PLoS ONE |
| title | Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin. |
| title_full | Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin. |
| title_fullStr | Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin. |
| title_full_unstemmed | Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin. |
| title_short | Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin. |
| title_sort | serum from calorie restricted rats activates vascular cell enos through enhanced insulin signaling mediated by adiponectin |
| url | http://europepmc.org/articles/PMC3271099?pdf=render |
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