Sustainable Exopolysaccharide Production by <i>Rhizobium viscosum</i> CECT908 Using Corn Steep Liquor and Sugarcane Molasses as Sole Substrates
Microbial exopolysaccharides (EPS) are promising alternatives to synthetic polymers in a variety of applications. Their high production costs, however, limit their use despite their outstanding properties. The use of low-cost substrates such as agro-industrial wastes in their production, can help to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/1/20 |
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| author | Eduardo J. Gudiña Márcia R. Couto Soraia P. Silva Elisabete Coelho Manuel A. Coimbra José A. Teixeira Lígia R. Rodrigues |
| author_facet | Eduardo J. Gudiña Márcia R. Couto Soraia P. Silva Elisabete Coelho Manuel A. Coimbra José A. Teixeira Lígia R. Rodrigues |
| author_sort | Eduardo J. Gudiña |
| collection | DOAJ |
| description | Microbial exopolysaccharides (EPS) are promising alternatives to synthetic polymers in a variety of applications. Their high production costs, however, limit their use despite their outstanding properties. The use of low-cost substrates such as agro-industrial wastes in their production, can help to boost their market competitiveness. In this work, an alternative low-cost culture medium (CSLM) was developed for EPS production by <i>Rhizobium viscosum</i> CECT908, containing sugarcane molasses (60 g/L) and corn steep liquor (10 mL/L) as sole ingredients. This medium allowed the production of 6.1 ± 0.2 g EPS/L, twice the amount produced in the standard medium (Syn), whose main ingredients were glucose and yeast extract. This is the first report of EPS production by <i>R. viscosum</i> using agro-industrial residues as sole substrates. EPS<sub>CSLM</sub> and EPS<sub>Syn</sub> exhibited a similar carbohydrate composition, mainly 4-linked galactose, glucose and mannuronic acid. Although both EPS showed a good fit to the Herschel–Bulkley model, EPS<sub>CSLM</sub> displayed a higher yield stress and flow consistency index when compared with EPS<sub>Syn</sub>, due to its higher apparent viscosity. EPS<sub>CSLM</sub> demonstrated its potential use in Microbial Enhanced Oil Recovery by enabling the recovery of nearly 50% of the trapped oil in sand-pack column experiments using a heavy crude oil. |
| first_indexed | 2024-03-09T09:42:57Z |
| format | Article |
| id | doaj.art-b8cce784265b47678b692dfaaec97d77 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T09:42:57Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b8cce784265b47678b692dfaaec97d772023-12-02T00:48:44ZengMDPI AGPolymers2073-43602022-12-011512010.3390/polym15010020Sustainable Exopolysaccharide Production by <i>Rhizobium viscosum</i> CECT908 Using Corn Steep Liquor and Sugarcane Molasses as Sole SubstratesEduardo J. Gudiña0Márcia R. Couto1Soraia P. Silva2Elisabete Coelho3Manuel A. Coimbra4José A. Teixeira5Lígia R. Rodrigues6CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalCEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalCEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalCEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalMicrobial exopolysaccharides (EPS) are promising alternatives to synthetic polymers in a variety of applications. Their high production costs, however, limit their use despite their outstanding properties. The use of low-cost substrates such as agro-industrial wastes in their production, can help to boost their market competitiveness. In this work, an alternative low-cost culture medium (CSLM) was developed for EPS production by <i>Rhizobium viscosum</i> CECT908, containing sugarcane molasses (60 g/L) and corn steep liquor (10 mL/L) as sole ingredients. This medium allowed the production of 6.1 ± 0.2 g EPS/L, twice the amount produced in the standard medium (Syn), whose main ingredients were glucose and yeast extract. This is the first report of EPS production by <i>R. viscosum</i> using agro-industrial residues as sole substrates. EPS<sub>CSLM</sub> and EPS<sub>Syn</sub> exhibited a similar carbohydrate composition, mainly 4-linked galactose, glucose and mannuronic acid. Although both EPS showed a good fit to the Herschel–Bulkley model, EPS<sub>CSLM</sub> displayed a higher yield stress and flow consistency index when compared with EPS<sub>Syn</sub>, due to its higher apparent viscosity. EPS<sub>CSLM</sub> demonstrated its potential use in Microbial Enhanced Oil Recovery by enabling the recovery of nearly 50% of the trapped oil in sand-pack column experiments using a heavy crude oil.https://www.mdpi.com/2073-4360/15/1/20Enhanced Oil Recoverypolymer floodingxanthan gumheavy oilpseudoplastic fluidHerschel–Bulkley model |
| spellingShingle | Eduardo J. Gudiña Márcia R. Couto Soraia P. Silva Elisabete Coelho Manuel A. Coimbra José A. Teixeira Lígia R. Rodrigues Sustainable Exopolysaccharide Production by <i>Rhizobium viscosum</i> CECT908 Using Corn Steep Liquor and Sugarcane Molasses as Sole Substrates Polymers Enhanced Oil Recovery polymer flooding xanthan gum heavy oil pseudoplastic fluid Herschel–Bulkley model |
| title | Sustainable Exopolysaccharide Production by <i>Rhizobium viscosum</i> CECT908 Using Corn Steep Liquor and Sugarcane Molasses as Sole Substrates |
| title_full | Sustainable Exopolysaccharide Production by <i>Rhizobium viscosum</i> CECT908 Using Corn Steep Liquor and Sugarcane Molasses as Sole Substrates |
| title_fullStr | Sustainable Exopolysaccharide Production by <i>Rhizobium viscosum</i> CECT908 Using Corn Steep Liquor and Sugarcane Molasses as Sole Substrates |
| title_full_unstemmed | Sustainable Exopolysaccharide Production by <i>Rhizobium viscosum</i> CECT908 Using Corn Steep Liquor and Sugarcane Molasses as Sole Substrates |
| title_short | Sustainable Exopolysaccharide Production by <i>Rhizobium viscosum</i> CECT908 Using Corn Steep Liquor and Sugarcane Molasses as Sole Substrates |
| title_sort | sustainable exopolysaccharide production by i rhizobium viscosum i cect908 using corn steep liquor and sugarcane molasses as sole substrates |
| topic | Enhanced Oil Recovery polymer flooding xanthan gum heavy oil pseudoplastic fluid Herschel–Bulkley model |
| url | https://www.mdpi.com/2073-4360/15/1/20 |
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