Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways Involved
In a prospective study, 48 fetuses were evaluated with Doppler ultrasound after 34 weeks and classified, according to the cerebroplacental ratio (CPR) and estimated fetal weight (EFW), into fetuses with normal growth and fetuses with late-onset fetal growth restriction (LO-FGR). Overexpression of mi...
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/1/293 |
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| author | Gabriela Loscalzo Julia Scheel José Santiago Ibañez-Cabellos Eva García-Lopez Shailendra Gupta José Luis García-Gimenez Salvador Mena-Mollá Alfredo Perales-Marín José Morales-Roselló |
| author_facet | Gabriela Loscalzo Julia Scheel José Santiago Ibañez-Cabellos Eva García-Lopez Shailendra Gupta José Luis García-Gimenez Salvador Mena-Mollá Alfredo Perales-Marín José Morales-Roselló |
| author_sort | Gabriela Loscalzo |
| collection | DOAJ |
| description | In a prospective study, 48 fetuses were evaluated with Doppler ultrasound after 34 weeks and classified, according to the cerebroplacental ratio (CPR) and estimated fetal weight (EFW), into fetuses with normal growth and fetuses with late-onset fetal growth restriction (LO-FGR). Overexpression of miRNAs from neonatal cord blood belonging to LO-FGR fetuses, was validated by real-time PCR. In addition, functional characterization of overexpressed miRNAs was performed by analyzing overrepresented pathways, gene ontologies, and prioritization of synergistically working miRNAs. Three miRNAs: miR-25-3p, miR-185-5p and miR-132-3p, were significantly overexpressed in cord blood of LO-FGR fetuses. Pathway and gene ontology analysis revealed over-representation of certain molecular pathways associated with cardiac development and neuron death. In addition, prioritization of synergistically working miRNAs highlighted the importance of miR-185-5p and miR-25-3p in cholesterol efflux and starvation responses associated with LO-FGR phenotypes. Evaluation of miR-25-3p; miR-132-3p and miR-185-5p might serve as molecular biomarkers for the diagnosis and management of LO-FGR; improving the understanding of its influence on adult disease. |
| first_indexed | 2024-03-10T03:38:57Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:38:57Z |
| publishDate | 2021-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b8d07fcdda9c41ecbc6c6d8cf096c5ed2023-11-23T11:37:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0123129310.3390/ijms23010293Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways InvolvedGabriela Loscalzo0Julia Scheel1José Santiago Ibañez-Cabellos2Eva García-Lopez3Shailendra Gupta4José Luis García-Gimenez5Salvador Mena-Mollá6Alfredo Perales-Marín7José Morales-Roselló8Instituto de Investigación Sanitaria La Fe, 46026 Valencia, SpainDepartment of Systems Biology and Bioinformatics, University Rostock, 18055 Rostock, GermanyEpiDisease S.L, Parc Científic, University of Valencia, 46980 Paterna, SpainEpiDisease S.L, Parc Científic, University of Valencia, 46980 Paterna, SpainDepartment of Systems Biology and Bioinformatics, University Rostock, 18055 Rostock, GermanyEpiDisease S.L, Parc Científic, University of Valencia, 46980 Paterna, SpainEpiDisease S.L, Parc Científic, University of Valencia, 46980 Paterna, SpainInstituto de Investigación Sanitaria La Fe, 46026 Valencia, SpainInstituto de Investigación Sanitaria La Fe, 46026 Valencia, SpainIn a prospective study, 48 fetuses were evaluated with Doppler ultrasound after 34 weeks and classified, according to the cerebroplacental ratio (CPR) and estimated fetal weight (EFW), into fetuses with normal growth and fetuses with late-onset fetal growth restriction (LO-FGR). Overexpression of miRNAs from neonatal cord blood belonging to LO-FGR fetuses, was validated by real-time PCR. In addition, functional characterization of overexpressed miRNAs was performed by analyzing overrepresented pathways, gene ontologies, and prioritization of synergistically working miRNAs. Three miRNAs: miR-25-3p, miR-185-5p and miR-132-3p, were significantly overexpressed in cord blood of LO-FGR fetuses. Pathway and gene ontology analysis revealed over-representation of certain molecular pathways associated with cardiac development and neuron death. In addition, prioritization of synergistically working miRNAs highlighted the importance of miR-185-5p and miR-25-3p in cholesterol efflux and starvation responses associated with LO-FGR phenotypes. Evaluation of miR-25-3p; miR-132-3p and miR-185-5p might serve as molecular biomarkers for the diagnosis and management of LO-FGR; improving the understanding of its influence on adult disease.https://www.mdpi.com/1422-0067/23/1/293late-onset fetal growth restrictionmiRNAnetwork analysisbioinformaticsfetal healthcerebroplacental ratio |
| spellingShingle | Gabriela Loscalzo Julia Scheel José Santiago Ibañez-Cabellos Eva García-Lopez Shailendra Gupta José Luis García-Gimenez Salvador Mena-Mollá Alfredo Perales-Marín José Morales-Roselló Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways Involved International Journal of Molecular Sciences late-onset fetal growth restriction miRNA network analysis bioinformatics fetal health cerebroplacental ratio |
| title | Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways Involved |
| title_full | Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways Involved |
| title_fullStr | Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways Involved |
| title_full_unstemmed | Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways Involved |
| title_short | Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways Involved |
| title_sort | overexpression of micrornas mir 25 3p mir 185 5p and mir 132 3p in late onset fetal growth restriction validation of results and study of the biochemical pathways involved |
| topic | late-onset fetal growth restriction miRNA network analysis bioinformatics fetal health cerebroplacental ratio |
| url | https://www.mdpi.com/1422-0067/23/1/293 |
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